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Human Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit

R&D Systems | Catalog # DC3L10

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL)

Sensitivity

8.15 pg/mL

Assay Range

62.5-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
View all Chitinase 3-like 1/YKL-40 ELISA Kits »

Product Summary for Human Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit

The Quantikine Human CHI3L1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure CHI3L1 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human CHI3L1 and antibodies raised against recombinant human CHI3L1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural CHI3L1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human CHI3L1.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human CHI3L1

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Sample Values

Serum/Plasma/Urine - Samples from apparently healthy volunteers were evaluated for the presence of human CHI3L1 in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean (ng/mL)Range (ng/mL)Standard Deviation (ng/mL)
Serum (n=36)36.015.9-93.520.6
EDTA plasma (n=36)27.710.9-79.117.5
Heparin plasma (n=36)26.810.7-72.115.7
Urine (n=11)0.640.14-1.820.49

Cell Culture Supernates:
HepG2 human hepatocellular carcinoma cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate until confluent. An aliquot of the cell culture supernate was removed, assayed for human CHI3L1, and measured 0.944 ng/mL.

MG-63 human osteosarcoma cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate for 4 days. An aliquot of the cell culture supernate was removed, assayed for human CHI3L1, and measured 3.04 ng/mL.

U-87 MG human glioblastoma/astrocytoma cells and SK-Mel-28 human malignant melanoma cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, 1 mM sodium pyruvate, and 100 μg/mL streptomycin sulfate until confluent. An aliquot of each cell culture supernate was removed and assayed for human CHI3L1. U-87 MG cells measured 16.1 ng/mL, and the SK-Mel-28 cells measured 0.071 ng/mL.

HASMC human aortic smooth muscle cells were cultured in Medium 231 supplemented with smooth muscle growth supplement until confluent. An aliquot of the cell culture supernate was removed, assayed for human CHI3L1, and measured 0.623 ng/mL.

BUD-8 human fibroblasts were cultured in 90% MEM with non-essential amino acids supplemented with 10% fetal bovine serum and 2 mM L-glutamine until confluent. An aliquot of the cell culture supernate was removed, assayed for human CHI3L1, and measured 4.31 ng/mL. 

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 566 1031 2202 606 1019 2212
Standard Deviation 26.7 44.4 102.6 32.1 59.1 153.1
CV% 4.7 4.3 4.7 5.3 5.8 6.9

Recovery for Human Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit

The recovery of CHI3L1 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 93-100
Urine (n=4) 105 90-115

Linearity

To assess the linearity of the assay, samples containing high concentrations of CHI3L1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Chitinase 3-like 1 ELISA Linearity

Scientific Data Images for Human Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit

Human Chitinase 3-like 1 ELISA Standard Curve

Human Chitinase 3-like 1 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Chitinase 3-like 1/YKL-40

Chitinase 3-like 1/YKL-40 (CHI3L1), also called BRP39 in mouse, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family although it does not show chitotriosidase activity. CHI3L1 is expressed by articular chondrocytes, synovial cells, activated monocyte-derived macrophages, neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells. CHI3L1 binds to chitin and heparins, and it enhances cell adhesion, proliferation and tumor angiogenesis. Circulating CHI3L1 is elevated during inflammation and connective tissue remodeling such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis. It is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary.

Alternate Names

CHI3L1, Chitinase 3 like 1, HCgp39, YKL-40

Entrez Gene IDs

1116 (Human); 12654 (Mouse); 102138036 (Cynomolgus Monkey)

Gene Symbol

CHI3L1

Additional Chitinase 3-like 1/YKL-40 Products

Product Documents for Human Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit

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  • Human Chitinase 3-like 1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 09/12/2020
  • Human Chitinase 3-like 1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Urine
    Verified Customer | Posted 06/13/2019
    Human Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit DC3L10
  • Human Chitinase 3-like 1 Quantikine ELISA Kit
    Name: Maria Garcia
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 10/15/2018
    Human Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit DC3L10
  • Human Chitinase 3-like 1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 08/29/2018

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Protocols

View specific protocols for Human Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit (DC3L10):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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