Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Chitotriosidase/CHIT1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Chitotriosidase/CHIT1
Ala22-Asn466
Accession # Q13231

Specificity

Detects human Chitotriosidase/CHIT1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Chitotriosidase/CHIT1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Chitotriosidase/CHIT1 (Catalog # 3559-GH), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Chitotriosidase/CHIT1 (Catalog # 3559-GH)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Chitotriosidase/CHIT1

Chitotriosidase encoded by the CHIT1 gene is a member of the chitinase family that is selectively expressed in activated tissue macrophages (1). It is distinct from another member, known as acidic mammalian chitinase (AMC) encoded by the CHIA gene and expressed mainly in the gastrointestinal tract and lung (2). Both CHIA and CHIT1 are secreted as 50 kDa proteins. In contrast to CHIA, CHIT1 is not stable under acidic pHs and can be processed into a C-terminally truncated 39 kDa form (2, 3). CHIT1 is the best biomarker in the monitoring of Gaucher disease among the three most commonly used markers that also include acid phosphatase and angiotensin-converting enzyme (ACE) (4). CHIT1 is also a specific marker of macrophage activation in acute ischemic stroke (5).

References

  1. Aguilera, B. et al. (2003) J. Biol. Chem. 278:40911.
  2. Boot, R.G. et al. (2001) J. Biol. Chem. 276:6770.
  3. Renkema, G.H. et al. (1997) Eur. J. Biochem. 244:279.
  4. Vellodi, A. et al. (2005) J. Inherit. Metab. Dis. 28:585.
  5. Sotgiu, S. et al. (2005) Eur. Neurol. 54:149.

Alternate Names

CHI3, CHIT1

Entrez Gene IDs

1118 (Human)

Gene Symbol

CHIT1

UniProt

Q13231

Additional Chitotriosidase/CHIT1 Products

Product Documents for Human Chitotriosidase/CHIT1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Chitotriosidase/CHIT1 Antibody

For research use only

Citations for Human Chitotriosidase/CHIT1 Antibody

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