Chromogranin B in Human Pituitary Gland. Chromogranin B was detected in immersion fixed paraffin-embedded sections of human pituitary gland using Mouse Anti-Human Chromogranin B Monoclonal Antibody (Catalog # MAB8868) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chromogranin B
Chromogranin B (CHGB), also known as Secretogranin-1, is a 673 amino acid neuroendocrine secretory granule protein which is cleaved into three fragments: PE-11 (aa 575-585), GAWK peptide (aa 440-513) and CCB peptide (617-673). Human CHGB was originally isolated from human pheochromocytoma tissue, and is expressed in normal and malignant neuroendocrine tissues. CHGB peptides play a role in hormonal regulation in pancreatic islets, hypothalamus and pituitary gland. CHGB peptides, (aa 314-332 and 350-365) have been identified in several endocrine tumors including, gastrinoma, thyroid carcinoma, insulinoma, pancreatic endocrine tumor and pituitary adenomas.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.