Human Chromogranin B Antibody

Catalog # Availability Size / Price Qty
MAB8868
MAB8868-SP
Chromogranin B in Human Pituitary Gland.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human Chromogranin B Antibody Summary

Species Reactivity
Human
Specificity
Detects human Chromogranin B in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 914334
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Chromogranin B
Met1-Gly677
Accession # P05060
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Chromogranin B in Human Pituitary Gland. View Larger

Chromogranin B in Human Pituitary Gland. Chromogranin B was detected in immersion fixed paraffin-embedded sections of human pituitary gland using Mouse Anti-Human Chromogranin B Monoclonal Antibody (Catalog # MAB8868) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chromogranin B

Chromogranin B (CHGB), also known as Secretogranin-1, is a 673 amino acid neuroendocrine secretory granule protein which is cleaved into three fragments: PE-11 (aa 575-585), GAWK peptide (aa 440-513) and CCB peptide (617-673). Human CHGB was originally isolated from human pheochromocytoma tissue, and is expressed in normal and malignant neuroendocrine tissues. CHGB peptides play a role in hormonal regulation in pancreatic islets, hypothalamus and pituitary gland. CHGB peptides, (aa 314-332 and 350-365) have been identified in several endocrine tumors including, gastrinoma, thyroid carcinoma, insulinoma, pancreatic endocrine tumor and pituitary adenomas.

Entrez Gene IDs
1114 (Human); 12653 (Mouse); 24259 (Rat)
Alternate Names
CgB; CHGB; chromogranin B (secretogranin 1); Chromogranin B; Chromogranin-B; SCG1; SCG1sgI; secretogranin B; Secretogranin I; secretogranin-1; SgI

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG1 Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Chromogranin B Antibody

There are currently no reviews for this product. Be the first to review Human Chromogranin B Antibody and earn rewards!

Have you used Human Chromogranin B Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review