Detects human Chymase in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse (rm) MCPT1 is observed and less than 1% cross-reactivity with recombinant human (rh) Granzyme-B, rhGranzyme-H, rmMCPT6 and rmMCPT7 is observed.
Polyclonal Sheep IgG
Mouse myeloma cell line NS0-derived recombinant human Chymase/CMA1 Gly20-Asn247 Accession # P23946
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human Chymase/CMA1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Chymase/CMA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4099) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Chymase/CMA1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Chymases are a group of chymotrypsin-like serine proteases secreted by mast cells (1). They are synthesized as inactive precursors containing a 2-residue propeptide, which needs to be removed by dipeptidyl peptidase I/cathepsin C for the enzymatic activity (2). Human Chymase encoded by the CMA1 gene is known to be involved in hypertention and heart failure through its ability to convert angiotensin I (Ang I) to angiotensin II (Ang II), which plays a key role in the regulation of arterial pressure (3). In addition, it is also important in physiological and pathological conditions including inflammation, fibrosis and processing of cytokines (4). Therefore, designing a specific inhibitor for Chymase activity has been a pharmacologic strategy to develop therapeutic agents.
Caughey, G.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. ed. p. 1531, Academic Press, San Diego.
Murakami, M. et al. (1995) J. Biol. Chem. 270:2218.
Miyazaki, M. and S. Takai (2006) J. Pharmacol. Sci. 100:391.
Nakajima, M. and N. Naya (2002) Jpn. J. Pharmacol. 90:206.
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