Human Chymotrypsin C/CTRC Antibody

Catalog # Availability Size / Price Qty
AF6907
AF6907-SP
Detection of Human Chymotrypsin C/CTRC by Western Blot.
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Human Chymotrypsin C/CTRC Antibody Summary

Species Reactivity
Human
Specificity
Detects human Chymotrypsin C/CTRC in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Chymotrypsin C/CTRC
Cys17-Leu268
Accession # Q99895
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Chymotrypsin C/CTRC by Western Blot. View Larger

Detection of Human Chymotrypsin C/CTRC by Western Blot. Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Chymotrypsin C/CTRC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6907) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Chymotrypsin C/CTRC at approximately 33-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1./P

Immunohistochemistry Chymotrypsin C/CTRC in Human Pancreas. View Larger

Chymotrypsin C/CTRC in Human Pancreas. Chymotrypsin C/CTRC was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human Chymotrypsin C/CTRC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6907) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter­stained with hematoxylin (blue). Specific staining was localized to cytoplasm of exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chymotrypsin C/CTRC

Chymotrypsin C (CTRC), also known as caldecrin, is a serine protease and a member of the peptidase S1 family. The CTRC gene encodes a serum calcium-decreasing factor that has chymotrypsin-like protease activity (1). CTRC has broad substrate specificity, but prefers to cleave on the carboxyl side of hydrophobic residues. The enzyme is expressed primarily in the pancreas, and is secreted into the digestive tract. CTRC is a key regulator of activation and degradation of cationic trypsinogen (2, 3). The inappropriate activation of cationic trypsinogen can contribute to the development of pancreatitis (2, 3). CTRC inhibits osteoclast differentiation by suppression of NFATc1 function by a mechanism independent of its proteolytic activity (4).

References
  1. Tomomura, A. et al. (1995) J. Biol. Chem. 270:30315.
  2. Szmola, R. and M. Sahin-Toth (2007) Proc. Natl. Acad. Sci. USA 104:11227.
  3. Nemoda, Z. and M. Sahin-Toth (2006) J. Biol. Chem. 281:11879.
  4. Hasegawa, H. et al. (2010) J. Biol. Chem. 285:25448.
Entrez Gene IDs
11330 (Human); 76701 (Mouse)
Alternate Names
Caldecrin; chymotrypsin C (caldecrin); Chymotrypsin C; chymotrypsin-C; CLCR; CLCRserum calcium decreasing factor; CTRC; EC 3.4.21; EC 3.4.21.2; ELA4; elastase 4; Elastase IV

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

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Secondary Antibodies

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