Human Chymotrypsin C/CTRC Antibody Summary
Accession # Q99895
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Chymotrypsin C/CTRC by Western Blot.
Detection of Human Chymotrypsin C/CTRC by Western Blot.Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Chymotrypsin C/CTRC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6907) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Chymotrypsin C/CTRC at approximately 33-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1./P
Chymotrypsin C/CTRC in Human Pancreas. Chymotrypsin C/CTRC was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human Chymotrypsin C/CTRC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6907) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chymotrypsin C/CTRC
Chymotrypsin C (CTRC), also known as caldecrin, is a serine protease and a member of the peptidase S1 family. The CTRC gene encodes a serum calcium-decreasing factor that has chymotrypsin-like protease activity (1). CTRC has broad substrate specificity, but prefers to cleave on the carboxyl side of hydrophobic residues. The enzyme is expressed primarily in the pancreas, and is secreted into the digestive tract. CTRC is a key regulator of activation and degradation of cationic trypsinogen (2, 3). The inappropriate activation of cationic trypsinogen can contribute to the development of pancreatitis (2, 3). CTRC inhibits osteoclast differentiation by suppression of NFATc1 function by a mechanism independent of its proteolytic activity (4).
- Tomomura, A. et al. (1995) J. Biol. Chem. 270:30315.
- Szmola, R. and M. Sahin-Toth (2007) Proc. Natl. Acad. Sci. USA 104:11227.
- Nemoda, Z. and M. Sahin-Toth (2006) J. Biol. Chem. 281:11879.
- Hasegawa, H. et al. (2010) J. Biol. Chem. 285:25448.
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