Human CIDEA Antibody

Catalog # Availability Size / Price Qty
Detection of Human CIDEA by Western Blot.
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Product Details
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Human CIDEA Antibody Summary

Species Reactivity
Detects human CIDEA in direct ELISAs and Western blots. In direct ELISAs, approximately 12% cross-reactivity with recombinant human CIDEC is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
Chinese hamster ovary cell line CHO-derived recombinant human CIDEA
Lys61-Arg162 (Val99Phe)
Accession # O60543
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human CIDEA antibody by Western Blot. View Larger

Detection of Human CIDEA by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CIDEA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7137) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CIDEA at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CIDEA

CIDEA (Cell death-Inducing DNA fragmentation factor-alpha -like Effector A) is a 24-27 kDa member of the CIDE family of molecules. It is expressed in human adipocytes and striated muscle, plus mouse brown adipocytes and hepatocytes, and appears to have at least two functions. In the cytoplasm of fat cells, CIDEA localizes to lipid droplets and ER, and promotes the formation of lipid droplets at the expense of lipolysis and AMPK activity. In the nucleus, CIDEA apparently binds to LXR, and is capable of inducing apoptosis. CIDEA undergoes O-linked glycosylation. When glycosylated, CIDEA is nuclear; when nonglycosylated, CIDEA is cytoplasmic. Human CIDEA is 219 amino acids (aa) in length, and contains one CIDE domain (aa 33-110) that potentially mediates dimerization. CIDEA reportedly homodimerizes, and heterodimerizes with CIDEB. There is one potential isoform variant that possesses a 46 aa substitution for aa 1-12. Over aa 61-162, human CIDEA shares 89% aa identity with mouse CIDEA.

Long Name
Cell Death-inducing DFFA-like Effector A
Entrez Gene IDs
1149 (Human); 12683 (Mouse); 291541 (Rat)
Alternate Names
cell death activator CIDE-A; cell death-inducing DFFA-like effector aCIDE-A; CIDEA

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