Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Dual RNAscope ISH-IHC Compatible, Immunocytochemistry, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 963420
View all Cytokeratin 19 Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant human Cytokeratin 19
Gln311-Lys370
Accession # P08727

Specificity

Detects human Cytokeratin 19 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Cytokeratin 19 Antibody

Cytokeratin 19 antibody in Human Breast Cancer Tissue Using Dual RNAscope®ISH and IHC.

Cytokeratin 19 in Human Breast Cancer Tissue Using Dual RNAscope®ISH and IHC.

Cytokeratin 19 mRNA (red) and protein (green) was detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer tissue probed with ACD RNAScope®Probe (Catalog # 310221) followed by immunohistochemistry using R&D Systems Mouse Anti-Human Cytokeratin 19 Monoclonal Antibody (Catalog # MAB35061) at 15ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC001). Tissue was stained using ACD RNAscope®2.5 HD Duplex Detection Reagents (Catalog # 322500).
Detection of Human Cytokeratin 19 antibody by Western Blot.

Detection of Human Cytokeratin 19 by Western Blot.

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, ZR-75 human breast cancer cell line, DU145 human prostate carcinoma cell line, MCF-7 human breast cancer cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Cytokeratin 19 Monoclonal Antibody (Catalog # MAB35061) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Cytokeratin 19 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cytokeratin 19 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

Cytokeratin 19 in MCF‑7 Human Cell Line.

Cytokeratin 19 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Cytokeratin 19 Monoclonal Antibody (Catalog # MAB35061) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Cytokeratin 19 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

Cytokeratin 19 in Human Breast Cancer Tissue.

Cytokeratin 19 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human Cytokeratin 19 Monoclonal Antibody (Catalog # MAB35061) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Cytokeratin 19 antibody in Human Gastric Cancer Tissue by Immunohistochemistry (IHC-P).

Cytokeratin 19 in Human Gastric Cancer Tissue.

Cytokeratin 19 was detected in immersion fixed paraffin-embedded sections of human gastric cancer tissue using Mouse Anti-Human Cytokeratin 19 Monoclonal Antibody (Catalog # MAB35061) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Cytokeratin 19 antibody in Human Papillary Thyroid Cancer Tissue by Immunohistochemistry (IHC-P).

Cytokeratin 19 in Human Papillary Thyroid Cancer Tissue.

Cytokeratin 19 was detected in immersion fixed paraffin-embedded sections of human papillary thyroid cancer tissue using Mouse Anti-Human Cytokeratin 19 Monoclonal Antibody (Catalog # MAB35061) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Cytokeratin 19 antibody in Human Lymphoma Tissue by Immunohistochemistry (IHC-P).

Cytokeratin 19 in Human Lymphoma Tissue.

Cytokeratin 19 was detected in immersion fixed paraffin-embedded sections of human lymphoma tissue using Mouse Anti-Human Cytokeratin 19 Monoclonal Antibody (Catalog # MAB35061) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human Cytokeratin 19 by Simple WesternTM.

Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and HT‑29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Cytokeratin 19 at approximately 50 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Cytokeratin 19 Monoclonal Antibody (Catalog # MAB35061). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunofluorescent Staining of iPSC-derived Pancreatic Duct-like Organoids

iPSC-derived pancreatic duct-like organoids were fixed and stained with Mouse Anti-Human KRT19 Monoclonal Antibody (Catalog # MAB35061; green) and a Rat Anti-Human Claudin-1 Monoclonal Antibody (Catalog # MAB4618; red), followed by secondary antibody staining with the NorthernLights™ 493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Polyclonal Antibody (Catalog # NL009) and NorthernLights 557-conjugated Goat Anti-Rat IgG Antigen Affinity-purified Polyclonal Antibody (Catalog # NL013). Cell nuclei were stained with DAPI (Catalog # 5748; blue) and the images were overlaid. Images were taken using a Leica THUNDER Imaging System (scale bar: 20 mm).

Applications for Human Cytokeratin 19 Antibody

Application
Recommended Usage

Dual RNAscope ISH-IHC Compatible

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue, human gastric cancer tissue, human papillary thyroid cancer tissue and human lymphoma tissue

Simple Western

50 µg/mL
Sample: MCF‑7 human breast cancer cell line and HT‑29 human colon adenocarcinoma cell line

Western Blot

0.25 µg/mL
Sample: HT‑29 human colon adenocarcinoma cell line, ZR‑75 human breast cancer cell line, DU145 human prostate carcinoma cell line, MCF‑7 human breast cancer cell line, and A431 human epithelial carcinoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB35061 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cytokeratin 19

Cytokeratin 19 (Keratin, type I cytoskeletal 19; also KRT-19, CK19 and Keratin-19) is a 40-45 kDa, acidic Class I keratin member of the intermediate filament family of proteins. Individual keratins are always expressed in tandem with a second keratin, and these are found in all epithelial cells. The class I KRT-19 heterodimerizes/polymerizes with 50-52 kDa class II KRT-8 (plus KRT-5 and -7) to form 8-10 nm filaments in epidermal stem cells, secretory gland (sweat; mammary; bile duct) simple epithelium, and neuroendocrine epidermal Merkel cells. It may represent a viable marker for skin stem cells. In skin, Cytokeratin 19 forms filaments in the fetal epithelium, and then progressively decreases with age, being virtually absent by age 17. Human Cytokeratin 19 is 400 amino acids (aa) in length. It contains an N-terminal "head" region (aa 1-79) and a subsequent "rod" region (aa 80-387), but is absent a typical C-terminal tail region. Cytokeratin 19 possesses at least 5 utilized phosphorylation sites plus one acetylated Lys residue. Based on other keratins, and the presence of an Asp at position 238, there may be caspase cleavage-generated isoforms. Full length human Cytokeratin 19 (aa 2-400) shares 82% aa sequence identity with mouse Cytokeratin 19.

Alternate Names

CK19, EndoC, K19, Krt19

Entrez Gene IDs

3880 (Human); 16669 (Mouse)

Gene Symbol

KRT19

UniProt

P08727

Additional Cytokeratin 19 Products

Product Documents for Human Cytokeratin 19 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Cytokeratin 19 Antibody

For research use only

Customer Reviews for Human Cytokeratin 19 Antibody (1)

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  • Human Cytokeratin 19 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: breast carcinoma
    Species: Human
    Verified Customer | Posted 11/23/2021
    Human Cytokeratin 19 Antibody MAB35061

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Protocols

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