Human CLEC14A Antibody

Catalog # Availability Size / Price Qty
AF4968
AF4968-SP
Human CLEC14A Antibody in Flow Cytometry
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Product Details
Citations (6)
FAQs
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Human CLEC14A Antibody Summary

Species Reactivity
Human
Specificity
Detects human CLEC14A in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) CLEC1, rhCLEC2, rhCLEC3B, and rhCLEC10A is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CLEC14A
Glu22-Ala397
Accession # Q86T13
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CLEC14A
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Clec-14A in HUVEC Human Cells by Flow Cytometry. View Larger

Detection of Clec-14A in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Sheep Anti-Human CLEC14A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4968, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).

Immunocytochemistry CLEC14A in HUVEC Human Cells. View Larger

CLEC14A in HUVEC Human Cells. CLEC14A was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Sheep Anti-Human CLEC14A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4968) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry CLEC14A in Human Breast Cancer Tissue. View Larger

CLEC14A in Human Breast Cancer Tissue. CLEC14A was detected in formalin fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human CLEC14A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4968) at 1.7 µg/mL overnight at
4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Universal (Catalog # CTS015). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC14A

CLEC14A (C-type lectin domain family 14 member A; also EGFR5) is a 51 kDa (predicted) member of the C-type lectin domain family of proteins. It is a type I transmembrane protein, apparently expressed in brain and about which little is known. Mature human CLEC14A is 469 amino acids in length. It contains a 376 aa extracellular region (aa 22-397) and a 72 aa cytoplasmic domain. The extracellular region shows one C-type lectin like domain (aa 32-175) and an EGF-like region (aa 245-287). Over aa 22-397, human CLEC14A shares 66% and 81% aa identity with mouse and canine CLEC14A, respectively.

Long Name
C-type Lectin Domain Family 14, Member A
Entrez Gene IDs
161198 (Human); 66864 (Mouse); 314148 (Rat)
Alternate Names
C14orf27; CEG1; chromosome 14 open reading frame 27; CLEC14A; ClECT and EGF-like domain containing protein; C-type lectin domain family 14 member A; C-type lectin domain family 14, member A; EGF R5; EGFR5; EGFR-5; Epidermal growth factor receptor 5

Product Datasheets

Citations for Human CLEC14A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Inhibition of VEGF-dependent angiogenesis and tumor angiogenesis by an optimized antibody targeting CLEC14a
    Authors: TK Kim, CS Park, J Jang, MR Kim, HJ Na, K Lee, HJ Kim, K Heo, BC Yoo, YM Kim, JW Lee, SJ Kim, ES Kim, DY Kim, K Cha, TG Lee, S Lee
    Mol Oncol, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. CLEC14a-HSP70-1A interaction regulates HSP70-1A-induced angiogenesis
    Authors: J Jang, MR Kim, TK Kim, WR Lee, JH Kim, K Heo, S Lee
    Sci Rep, 2017;7(1):10666.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Human antibodies targeting the C-type lectin-like domain of the tumor endothelial cell marker clec14a regulate angiogenic properties in vitro.
    Authors: Ki, M K, Jeoung, M H, Choi, J R, Rho, S-S, Kwon, Y-G, Shim, H, Chung, J, Hong, H J, Song, B D, Lee, S
    Oncogene, 2013;32(48):5449-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Identification and angiogenic role of the novel tumor endothelial marker CLEC14A.
    Authors: Mura M, Swain RK, Zhuang X
    Oncogene, 2012;31(3):293-305.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Blocking CLEC14A-MMRN2 binding inhibits sprouting angiogenesis and tumour growth.
    Authors: Noy P, Lodhia P, Khan K, Zhuang X, Ward D, Verissimo A, Bacon A, Bicknell R
    Oncogene, 0;34(47):5821-31.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  6. Sprouting angiogenesis is regulated by shedding of the C-type lectin family 14, member A (CLEC14A) ectodomain, catalyzed by rhomboid-like 2 protein (RHBDL2).
    Authors: Noy P, Swain R, Khan K, Lodhia P, Bicknell R
    FASEB J, 0;30(6):2311-23.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot

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