Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Contactin-3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Contactin-3
Pro26-Pro992
Accession # Q9P232

Specificity

Detects human Contactin-3 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Contactin-3 is observed, and less than 5% cross-reactivity with recombinant human (rh) Contactin-4 and rhContactin-5 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Contactin‑3 Antibody

Contactin-3 antibody in Human Brain by Immunohistochemistry (IHC-P).

Contactin‑3 in Human Brain.

Contactin-3 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Sheep Anti-Human Contactin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7125) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Contactin‑3 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Contactin-3

Contactin-3, also known as BIG-1, CNTN3 and PANG, is a 150 kDa member of the TAG/F3 subgroup of Contactin neuronal adhesion proteins (1, 2). Mature human Contactin-3 consists of six immunoglobulin-like domains, a flexible linker region, four fibronectin type III domains, and a GPI anchor (3, 4). Human Contactin-3 shares 92% aa sequence identity with mouse and rat Contactin-3. Contactin-3 is primarily expressed in the brain in discrete regions including the frontal lobe, occipital lobe, cerebellum, amygdala, and hippocampus (5, 6). Alternative splicing in rat may generate a soluble isoform (4). Mouse plasmacytoma cell lines produce multiple transcripts of Contactin-3 due to the presence of IAP proviruses (3). Immobilized Contactin-3 promotes neurite outgrowth from rat hippocampal neurons (4).

References

  1. Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
  2. Denisenko-Nehrbass, N. et al. (2002) J. Physiol. 96:99.
  3. Connelly, M.A. et al. (1994) Proc. Natl. Acad. Sci. 91:1337.
  4. Yoshihara, Y. et al. (1994) Neuron 13:415.
  5. Kamei, Y. et al. (2000) Genomics 69:113.
  6. Yoshihara, Y. et al. (1995) J. Neurobiol. 28:51.

Alternate Names

BIG-1, CNTN3, Contactin3, PANG, PCS

Entrez Gene IDs

5067 (Human); 18488 (Mouse); 54279 (Rat)

Gene Symbol

CNTN3

UniProt

Q9P232

Additional Contactin-3 Products

Product Documents for Human Contactin‑3 Antibody

Certificate of Analysis

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Product Specific Notices for Human Contactin‑3 Antibody

For research use only

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Protocols

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