Human Crossveinless-2/CV-2 Antibody

Catalog #: AF1956
1 Citation Datasheet / COA / SDS

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AF1956
AF1956-SP

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Crossveinless‑2/CV‑2 in HUVEC Human Cells.

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Human Crossveinless-2/CV-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Crossveinless‑2/CV‑2 in direct ELISAs and Western blots. In these formats, approximately 10% cross-reactivity with recombinant mouse CV-2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Crossveinless‑2/CV‑2 (R&D Systems, Catalog # 1956-CV)
Val34-Arg685
Accession # Q8N8U9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Crossveinless‑2/CV‑2 (Catalog # 1956-CV)

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Crossveinless‑2/CV‑2 in HUVEC Human Cells. Crossveinless-2/CV-2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Human Crossveinless-2/CV-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1956) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Crossveinless-2/CV-2

Crossveinless-2 (CV-2), also known as bone morphogenetic protein-binding endothelial cell precursor-derived regulator (BMPER), is a secreted chordin-like protein that modulates the BMP signaling pathway (1‑3). Human CV-2 is synthesized as a 685 amino acid (aa) residue precursor protein with a putative 39 aa signal peptide, five tandem chordin-like cysteine-rich (CR) domains, a partial von Willebrand factor type D domain (vWD), and a carboxyl trypsin inhibitor-like cysteine-rich domain (TIL) (1, 4). Secreted CV-2 is reported to be proteolytically cleaved to generate two fragments that are disulfide-linked (1, 2). The GDPH sequence is conserved in CV-2 from other species. It is also found in multiple proteins that undergo a similar type of cleavage (5). Human CV-2 message is detected in many tissues, with the highest expression detected in adult brain and adult and fetal lung (1). It is also expressed in flk-1⁺ endothelial cell precursors and in primary chondrocytes (2). During embryonic development, CV-2 is expressed in regions of high BMP signaling, such as the posterior primitive streak and the ventral tail bud (4). Human CV-2 shares 92% and 34% aa sequence identity with the mouse and Drosophila homologs, respectively (1, 4). Results from biochemical experiments using recombinant CV-2 show that CV-2 directly interacts with BMP-2, -4, and -6 to antagonize BMP signaling, which can regulate a wide range of differentiation processes (1, 2). In contrast, genetic data from Drosophila suggest that CV-2 potentiates BMP-signaling (6). It is possible that like TSG, CV-2 can positively and negatively modulate BMP signal transduction depending on the cell context (7).

References

Binnerts, M.E. et al. (2004) Biochem Biophys Res Commun. 315:272.
Moser, M. et al. (2003) Mol Cell Biol. 23:5664.
Garcia-Abreu, J. et al. (2002) Gene, 287: 39.
Coffinier, C. et al. (2002) Mech Dev. 119:S179.
Lidell, M.E. et al. (2003) J. Biol. Chem. 278:13944.
Conley, C.A. et al. (2000) Development 127:3947.
Kamimura, M. et al. (2004) Developmental Dynamics 230:434.

Long Name

BMP-binding Endothelial Regulator Protein

Entrez Gene IDs

168667 (Human); 73230 (Mouse)

Alternate Names

BMP binding endothelial regulator; BMP-binding endothelial regulator precursor protein; BMP-binding endothelial regulator protein; BMPER; Bone morphogenetic protein-binding endothelial cell precursor-derived regulator; CRIM3; crossveinless 2; Crossveinless-2; CV2; CV-2; hCV2; KIAA1965; Protein crossveinless-2

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human Crossveinless-2/CV-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

BMP activity controlled by BMPER regulates the proinflammatory phenotype of endothelium.
Authors: Helbing T, Rothweiler R, Ketterer E, Goetz L, Heinke J, Grundmann S, Duerschmied D, Patterson C, Bode C, Moser M
Blood, 2011;118(18):5040-9.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot