Human Crossveinless-2/CV-2 Antibody

Catalog # Availability Size / Price Qty
AF1956
AF1956-SP
Human Crossveinless-2/CV-2 Antibody in Immunocytochemistry
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Citations (1)
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Human Crossveinless-2/CV-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Crossveinless‑2/CV‑2 in direct ELISAs and Western blots. In these formats, approximately 10% cross-reactivity with recombinant mouse CV-2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Crossveinless‑2/CV‑2 (R&D Systems, Catalog # 1956-CV)
Val34-Arg685
Accession # Q8N8U9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Crossveinless‑2/CV‑2 (Catalog # 1956-CV)
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Crossveinless‑2/CV‑2 in HUVEC Human Cells. View Larger

Crossveinless‑2/CV‑2 in HUVEC Human Cells. Crossveinless‑2/CV‑2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Human Crossveinless‑2/CV‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1956) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Crossveinless-2/CV-2

Crossveinless-2 (CV-2), also known as bone morphogenetic protein-binding endothelial cell precursor-derived regulator (BMPER), is a secreted chordin-like protein that modulates the BMP signaling pathway (1‑3). Human CV-2 is synthesized as a 685 amino acid (aa) residue precursor protein with a putative 39 aa signal peptide, five tandem chordin-like cysteine-rich (CR) domains, a partial von Willebrand factor type D domain (vWD), and a carboxyl trypsin inhibitor-like cysteine-rich domain (TIL) (1, 4). Secreted CV-2 is reported to be proteolytically cleaved to generate two fragments that are disulfide-linked (1, 2). The GDPH sequence is conserved in CV-2 from other species. It is also found in multiple proteins that undergo a similar type of cleavage (5). Human CV-2 message is detected in many tissues, with the highest expression detected in adult brain and adult and fetal lung (1). It is also expressed in flk-1+ endothelial cell precursors and in primary chondrocytes (2). During embryonic development, CV-2 is expressed in regions of high BMP signaling, such as the posterior primitive streak and the ventral tail bud (4). Human CV-2 shares 92% and 34% aa sequence identity with the mouse and Drosophila homologs, respectively (1, 4). Results from biochemical experiments using recombinant CV-2 show that CV-2 directly interacts with BMP-2, -4, and -6 to antagonize BMP signaling, which can regulate a wide range of differentiation processes (1, 2). In contrast, genetic data from Drosophila suggest that CV-2 potentiates BMP-signaling (6). It is possible that like TSG, CV-2 can positively and negatively modulate BMP signal transduction depending on the cell context (7).

References
  1. Binnerts, M.E. et al. (2004) Biochem Biophys Res Commun. 315:272.  
  2. Moser, M. et al. (2003) Mol Cell Biol. 23:5664. 
  3. Garcia-Abreu, J. et al. (2002) Gene, 287: 39. 
  4. Coffinier, C. et al. (2002) Mech Dev. 119:S179.
  5. Lidell, M.E. et al. (2003) J. Biol. Chem. 278:13944.
  6. Conley, C.A. et al. (2000) Development 127:3947.
  7. Kamimura, M. et al. (2004) Developmental Dynamics 230:434.
Long Name
BMP-binding Endothelial Regulator Protein
Entrez Gene IDs
168667 (Human); 73230 (Mouse)
Alternate Names
BMP binding endothelial regulator; BMP-binding endothelial regulator precursor protein; BMP-binding endothelial regulator protein; BMPER; Bone morphogenetic protein-binding endothelial cell precursor-derived regulator; CRIM3; crossveinless 2; Crossveinless-2; CV2; CV-2; hCV2; KIAA1965; Protein crossveinless-2

Product Datasheets

Citation for Human Crossveinless-2/CV-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. BMP activity controlled by BMPER regulates the proinflammatory phenotype of endothelium.
    Authors: Helbing T, Rothweiler R, Ketterer E, Goetz L, Heinke J, Grundmann S, Duerschmied D, Patterson C, Bode C, Moser M
    Blood, 2011;118(18):5040-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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