Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Neutralization, Immunocytochemistry, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CXCL1/GRO alpha/KC/CINC-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CXCL1/GRO alpha /KC/CINC-1 (R&D Systems, Catalog # 275-GR)
Ala35-Asn107
Accession # P09341

Specificity

Detects human CXCL1/GRO alpha /KC/CINC-1 in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) GRO beta and rhGRO gamma is observed, less than 25% cross-reactivity with recombinant rat (rr) CINC-1 is observed, less than 15% cross-reactivity with recombinant mouse (rm) KC is observed and no cross-reactivity with rhNAP-2 or rhPF-4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CXCL1/GRO alpha /KC/CINC‑1 Antibody

Detection of Recombinant Human CXCL1/GROa/KC/CINC-1 antibody by Western Blot.

Detection of Recombinant Human CXCL1/GRO alpha /KC/CINC‑1 by Western Blot.

Western blot shows 25 ng of Recombinant Human CXCL1/GROa/KC/CINC-1 (275-GR), Recombinant Mouse CXCL1/GROa/KC/CINC-1 aa 20-96 (453-KC), Recombinant Rat CXCL1/GROa/KC/CINC-1 (515-CN), Recombinant Human CXCL2/GRO beta /MIP-2/CINC-3 aa 35-107 (276-GB), Recombinant Human CXCL3/GRO gamma/CINC-2/DCIP-1 (277-GG), Recombinant Human CXCL7/NAP-2 (393-NP), and Recombinant Human CXCL4/PF4 (795-P4). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human CXCL1/GROa/KC/CINC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF275) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for CXCL1/GROa/KC/CINC-1 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Chemotaxis Induced by CXCL1/GRO alpha and Neutralization by Human CXCL1/GRO alpha Antibody.

Chemotaxis Induced by CXCL1/GRO alpha and Neutralization by Human CXCL1/GRO alpha Antibody.

Recombinant Human CXCL1/GROa (275-GR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CXCL1/GROa (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Human CXCL1/GROa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF275). The ND50 is typically 0.03-0.15 µg/mL.

Applications for Human CXCL1/GRO alpha /KC/CINC‑1 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human CXCL1/GRO alpha /KC/CINC‑1 (Catalog # 275-GR)

Neutralization

Measured by its ability to neutralize CXCL1/GRO alpha /KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.03-0.15 µg/mL in the presence of 0.01 µg/mL Recombinant Human CXCL1/GRO alpha /KC/CINC‑1.

Reviewed Applications

Read 6 reviews rated 4.7 using AF275 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL1/GRO alpha/KC/CINC-1

The gene for CXCL1/GRO alpha was initially discovered in hamster cells, using subtractive hybridization techniques, as a message that is over-expressed in tumorigenic cells and in normal cells during growth stimulation. The hamster cDNA was cloned and used as a probe for the subsequent cloning of the human GRO cDNA. Independently, a cDNA encoding a secreted protein with melanoma growth stimulating activity (MGSA) was also cloned from a human melanoma cell line and found to be identical to GRO. In addition to the initially cloned GRO gene, now designated CXCL1, two additional GRO genes, GRO beta or MIP-2 alpha and GRO gamma or MIP‑2 beta, which shared 90% and 86% amino acid sequence homology, respectively, with CXCL1, have been identified. All three human GROs are members of the alpha (C-X-C) subfamily of chemokines. The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes, and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B. The rat homolog of human CXCL1, CINC, is much more active than human CXCL1 on rat neutrophils, suggesting that this cytokine may have selective species specificity.

Alternate Names

CINC-1, CINC1, GRO alpha, KC, MGSA-alpha

Entrez Gene IDs

2919 (Human); 14825 (Mouse); 81503 (Rat)

Gene Symbol

CXCL1

UniProt

P09341

Additional CXCL1/GRO alpha/KC/CINC-1 Products

Product Documents for Human CXCL1/GRO alpha /KC/CINC‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CXCL1/GRO alpha /KC/CINC‑1 Antibody

For research use only

Citations for Human CXCL1/GRO alpha /KC/CINC‑1 Antibody

Customer Reviews for Human CXCL1/GRO alpha /KC/CINC‑1 Antibody (6)

4.7 out of 5
6 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Showing  1 - 5 of 6 reviews Showing All
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  • Human CXCL1/GRO alpha /KC/CINC-1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Breast cancer cells
    Species: Human
    Verified Customer | Posted 11/12/2025
    Human CXCL1/GRO alpha /KC/CINC‑1 Antibody AF275
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Human CXCL1/GRO alpha /KC/CINC-1 Antibody
    Name: Katie Myers
    Application: ELISA
    Sample Tested: Serum
    Species: Primate
    Verified Customer | Posted 11/28/2022
    Worked as a capture in our NHP ELISA
    Human CXCL1/GRO alpha /KC/CINC‑1 Antibody AF275
  • Human CXCL1/GRO alpha /KC/CINC-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Raw 246.7 cells
    Species: Human
    Verified Customer | Posted 04/04/2020
    Human CXCL1/GRO alpha /KC/CINC‑1 Antibody AF275
  • Human CXCL1/GRO alpha /KC/CINC-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Jurkat human acute T cell leukemia cell line and iDC human immature myeloid dendritic cells
    Species: Human
    Verified Customer | Posted 10/17/2018
    Human CXCL1/GRO alpha /KC/CINC‑1 Antibody AF275
  • Human CXCL1/GRO alpha /KC/CINC-1 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 08/17/2018
    CXCL1 levels were measured in plasma samples using this antibody.
    Human CXCL1/GRO alpha /KC/CINC‑1 Antibody AF275
  • Human CXCL1/GRO alpha /KC/CINC-1 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/22/2017
    AF275 was used as both the capture and detection antibody in a sandwich ELISA for GRO-alpha. The immunoassay standard was 275-GR.
    Human CXCL1/GRO alpha /KC/CINC‑1 Antibody AF275

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