Human CXCL10/IP-10/CRG-2 Antibody [Biotin]

R&D Systems | Catalog # BAF266

R&D Systems
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA Detection (Matched Antibody Pair), Intracellular Staining by Flow Cytometry

Cited:

Immunocytochemistry, ELISA Detection, ELISA Development, ELISA Development (Detection), Luminex Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all CXCL10/IP-10/CRG-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CXCL10/IP-10

Specificity

Detects human CXCL10/IP-10/CRG-2 in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant mouse CRG‑2, recombinant human (rh) Lymphotactin, rhGRO alpha, and rhMIG is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CXCL10/IP-10/CRG-2 Antibody [Biotin]

Detection of CXCL10/IP-10/CRG02 in Human PBMC by Flow Cytometry.

Human PBMC treated (filled histogram) and untreated (open histogram) with 75 ng/mL rhIFNy and monensin overnight were stained with Goat anti-human CXCL10/IP-10/CRG02 biotin (BAF266) followed by Phycoerythrin-conjugated Streptavidin (F0040). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.
Human CXCL10 / IP-10 / CRG-2 ELISA Standard Curve

Human CXCL10 / IP-10 / CRG-2 ELISA Standard Curve

Recombinant Human CXCL10/IP‑10/CRG‑2 (Catalog # 266-IP) was serially diluted and captured by Mouse Anti-Human CXCL10/IP‑10/CRG‑2 Monoclonal Antibody (Catalog # MAB266) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL10/IP‑10/CRG‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-266-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human CXCL10/IP-10/CRG-2 Antibody [Biotin]

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood monocytes treated with Recombinant Human IFN‑ gamma (Catalog # 285‑IF), fixed with paraformaldehyde, and permeabilized with saponin

Human CXCL10/IP-10 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human CXCL10/IP‑10/CRG‑2 Antibody (Catalog # MAB266)
  • Capture Reagent: Human CXCL10/IP‑10/CRG‑2 Antibody (Catalog # MAB266R)
  • Standard: Recombinant Human CXCL10/IP-10 Protein (Catalog # 266-IP)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using BAF266 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CXCL10/IP-10/CRG-2

CXCL10 was originally identified as an IFN-gamma -inducible gene in monocytes, fibroblasts, and endothelial cells. It has since been shown that CXCL10 mRNA is also induced by LPS, IL-1 beta, TNF-alpha, IL-12, and viruses. Additional cell types that have been shown to express CXCL10 include activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. CXCL10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human CXCL10, CRG-2, has been cloned and shown to share approximately 67% amino acid (aa) sequence identity with human CXCL10. Human CXCL10 cDNA encodes a 98 aa residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted protein. The amino acid sequence of CXCL10 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes. CXCL10 has been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent anti-tumor effect. A chemokine receptor specific for CXCL10 and MIG has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

References

  1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
  2. Wang, X. et al. (1996) J. Biol. Chem. 271:24286.

Alternate Names

CRG-2, CRG2, IP-10

Entrez Gene IDs

3627 (Human); 15945 (Mouse)

Gene Symbol

CXCL10

Additional CXCL10/IP-10/CRG-2 Products

Product Documents for Human CXCL10/IP-10/CRG-2 Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CXCL10/IP-10/CRG-2 Antibody [Biotin]

For research use only

Citations for Human CXCL10/IP-10/CRG-2 Antibody [Biotin]

Customer Reviews for Human CXCL10/IP-10/CRG-2 Antibody [Biotin] (2)

4.5 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human CXCL10/IP-10/CRG-2 Antibody [Biotin]?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Human CXCL10/IP‑10/CRG‑2 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 08/17/2018
    Human CXCL10/IP-10/CRG-2 Antibody [Biotin] BAF266
  • Human CXCL10/IP‑10/CRG‑2 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/17/2017
    Sandwich ELISA using MAB266 on solid phase and BAF266 as detection.
    Human CXCL10/IP-10/CRG-2 Antibody [Biotin] BAF266

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies