Human CXCL10/IP‑10/CRG‑2 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF266

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Product Details
Citations (16)
FAQs
Supplemental Products
Reviews (2)

Human CXCL10/IP‑10/CRG‑2 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCL10/IP-10/CRG-2 in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant mouse CRG‑2, recombinant human (rh) Lymphotactin, rhGRO alpha, and rhMIG is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CXCL10/IP-10
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Human peripheral blood monocytes treated with Recombinant Human IFN‑ gamma (Catalog # 285‑IF), fixed with paraformaldehyde, and permeabilized with saponin

Human CXCL10/IP-10 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human CXCL10/IP‑10/CRG‑2 Antibody (Catalog # MAB266R)

Capture Reagent: Human CXCL10/IP‑10/CRG‑2 Antibody (Catalog # MAB266)

Standard: Recombinant Human CXCL10/IP-10 Protein (Catalog # 266-IP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL10/IP-10/CRG-2

CXCL10 was originally identified as an IFN-gamma -inducible gene in monocytes, fibroblasts, and endothelial cells. It has since been shown that CXCL10 mRNA is also induced by LPS, IL-1 beta, TNF-alpha, IL-12, and viruses. Additional cell types that have been shown to express CXCL10 include activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. CXCL10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human CXCL10, CRG-2, has been cloned and shown to share approximately 67% amino acid (aa) sequence identity with human CXCL10. Human CXCL10 cDNA encodes a 98 aa residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted protein. The amino acid sequence of CXCL10 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes. CXCL10 has been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent anti-tumor effect. A chemokine receptor specific for CXCL10 and MIG has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

References
  1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
  2. Wang, X. et al. (1996) J. Biol. Chem. 271:24286.
Entrez Gene IDs
3627 (Human); 15945 (Mouse)
Alternate Names
C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10

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Citations for Human CXCL10/IP‑10/CRG‑2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Immune response dynamics in COVID-19 patients to SARS-CoV-2 and other human coronaviruses
    Authors: R Ravindran, C McReynolds, J Yang, BD Hammock, A Ikram, A Ali, A Bashir, T Zohra, WLW Chang, DJ Hartigan-O, HH Rashidi, IH Khan
    PLoS ONE, 2021;16(7):e0254367.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Detection
  2. Immune-Stimulatory Effects of Curcumin on the Tumor Microenvironment in Head and Neck Squamous Cell Carcinoma
    Authors: C Kötting, L Hofmann, R Lotfi, D Engelhardt, S Laban, PJ Schuler, TK Hoffmann, C Brunner, MN Theodoraki
    Cancers, 2021;13(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Detection
  3. Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus
    Sci Rep, 2016;6(0):29909.
    Species: Human
    Sample Types: Serum
  4. Allergen challenge in vivo alters rhinovirus-induced chemokine secretion from human airway macrophages.
    Authors: Karta, Maya R, Wickert, Lisa E, Curran, Colleen, Gavala, Monica L, Denlinger, Loren C, Gern, James E, Bertics, Paul J
    J Allergy Clin Immunol, 2014;133(4):1227-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development (Detection)
  5. Human rhinovirus-induced ISG15 selectively modulates epithelial antiviral immunity.
    Authors: Zaheer R, Wiehler S, Hudy M, Traves S, Pelikan J, Leigh R, Proud D
    Mucosal Immunol, 2014;7(5):1127-38.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  6. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  7. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007;329(1):214-8.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  8. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
    Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
    Clin. Chem., 2007;53(11):2010-2.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  9. Eotaxin-2 and colorectal cancer: a potential target for immune therapy.
    Authors: Cheadle EJ, Riyad K, Subar D, Rothwell DG, Ashton G, Batha H, Sherlock DJ, Hawkins RE, Gilham DE
    Clin. Cancer Res., 2007;13(19):5719-28.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  10. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007;109(10):4272-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  11. Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha.
    Authors: Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA
    J. Immunol., 2007;179(7):4840-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  12. Combination therapy: Synergistic suppression of virus-induced chemokines in airway epithelial cells.
    Authors: Edwards MR, Johnson MW, Johnston SL
    Am. J. Respir. Cell Mol. Biol., 2006;34(5):616-24.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  13. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006;120(2):220-31.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  14. Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
    Authors: Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
    Blood, 2004;104(2):314-20.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  15. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002;169(9):5244-51.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  16. Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
    Authors: Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
    J. Immunol., 2002;169(11):6515-21.
    Species: Human
    Sample Types: BALF
    Applications: ELISA Development

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Reviews for Human CXCL10/IP‑10/CRG‑2 Biotinylated Antibody

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Human CXCL10/IP‑10/CRG‑2 Biotinylated Antibody
By Anonymous on 08/17/2018
Application: ELISA Sample Tested: EDTA Plasma Species: Human

Human CXCL10/IP‑10/CRG‑2 Biotinylated Antibody
By Anonymous on 11/17/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

Sandwich ELISA using MAB266 on solid phase and BAF266 as detection.