Human CXCL10/IP-10/CRG-2 Antibody

(28 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CXCL10/IP-10/CRG-2 in ELISAs and Western blots. In Western blots, does not cross-react with recombinant human CXCL1, 2, 3, 5, 8, 9, 12/SDF-1 beta, recombinant mouse CXCL1, or 2.
  • Source
    Monoclonal Mouse IgG1 Clone # 33036
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human CXCL10/IP-10/CRG-2
    Val22-Pro98
    Accession # P02778.2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human CXCL10/IP‑10/CRG‑2 (Catalog # 266-IP)
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    Human peripheral blood monocytes treated with Recombinant Human IFN‑ gamma (Catalog # 285‑IF), fixed with paraformaldehyde, and permeabilized with saponin
    • Human CXCL10/IP-10 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human CXCL10/IP‑10/CRG‑2 Antibody (Catalog # MAB266)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human CXCL10/IP‑10/CRG‑2 Biotinylated Antibody (Catalog # BAF266)
  • ELISA Standard
     
    Recombinant Human CXCL10/IP-10 Protein (Catalog # 266-IP)
  • Neutralization
    Measured by its ability to neutralize CXCL10/IP‑10/CRG‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR3. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 0.2 µg/mL Recombinant Human CXCL10/IP‑10/CRG‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CXCL10/IP‑10 and Neutralization by Human CXCL10/IP‑10 Antibody. Recombinant Human CXCL10/
IP‑10 (Catalog # 266‑IP) chemo­attracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL10/
IP‑10 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL10/IP‑10 Mono­clonal Antibody (Catalog # MAB266). The ND50 is typically 0.5-2.0 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL10/IP-10/CRG-2

CXCL10 was originally identified as an IFN-gamma -inducible gene in monocytes, fibroblasts and endothelial cells. It has since been shown that CXCL10 mRNA is also induced by LPS, IL-1 beta, TNF-alpha, IL-12, and viruses. Additional cell types that have been shown to express CXCL10 include activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. CXCL10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human CXCL10, CRG-2, has been cloned and shown to share approximately 67% amino acid sequence identity with human CXCL10. Human CXCL10 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted protein. The amino acid sequence of CXCL10 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes. CXCL10 has been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent antitumor effect. A chemokine receptor specific for CXCL10 and Mig has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

  • References:
    1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
    2. Wang, X. et al. (1996) J. Biol. Chem. 271:24286.
  • Entrez Gene IDs:
    3627 (Human); 15945 (Mouse)
  • Alternate Names:
    C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus
    Sci Rep, 2016;6(0):29909.
    Species: Human
    Sample Type: Serum
  2. Escherichia coli Pyruvate Dehydrogenase Complex Is an Important Component of CXCL10-Mediated Antimicrobial Activity.
    Authors: Schutte K, Fisher D, Burdick M, Mehrad B, Mathers A, Mann B, Nakamoto R, Hughes M
    Infect Immun, 2015;84(1):320-8.
    Species: Human
    Sample Type: Recombinant Protein
    Application: immunoassay
  3. Allergen challenge in vivo alters rhinovirus-induced chemokine secretion from human airway macrophages.
    Authors: Karta, Maya R, Wickert, Lisa E, Curran, Colleen, Gavala, Monica L, Denlinger, Loren C, Gern, James E, Bertics, Paul J
    J Allergy Clin Immunol, 2014;133(4):1227-30.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Capture
  4. Human rhinovirus-induced ISG15 selectively modulates epithelial antiviral immunity.
    Authors: Zaheer R, Wiehler S, Hudy M, Traves S, Pelikan J, Leigh R, Proud D
    Mucosal Immunol, 2014;7(5):1127-38.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Capture
  5. Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation.
    Authors: Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R
    BMC Cancer, 2014;14(0):118.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Cetuximab-activated natural killer and dendritic cells collaborate to trigger tumor antigen-specific T-cell immunity in head and neck cancer patients.
    Authors: Srivastava R, Lee S, Andrade Filho P, Lord C, Jie H, Davidson H, Lopez-Albaitero A, Gibson S, Gooding W, Ferrone S, Ferris R
    Clin Cancer Res, 2013;19(7):1858-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  7. Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
    J. Exp. Med., 2012;209(10):1813-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  8. Pathological study of archival lung tissues from five fatal cases of avian H5N1 influenza in Vietnam.
    Authors: Nakajima N, Van Tin N, Sato Y, Thach H, Katano H, Diep P, Kumasaka T, Thuy N, Hasegawa H, San L, Kawachi S, Liem N, Suzuki K, Sata T
    Mod Pathol, 2012;26(3):357-69.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  9. Evidence for a pathophysiological role of keratinocyte-derived type III interferon (IFN-lambda) in cutaneous lupus erythematosus.
    Authors: Zahn S, Rehkamper C, Kummerer BM, Ferring-Schmidt S, Bieber T, Tuting T, Wenzel J
    J. Invest. Dermatol., 2011;131(1):133-40.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  10. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010;184(6):2886-98.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  11. Expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome.
    Authors: Yoon KC, Park CS, You IC, Choi HJ, Lee KH, Im SK, Park HY, Pflugfelder SC
    Invest. Ophthalmol. Vis. Sci., 2010;51(2):643-50.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  12. Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
    Authors: Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F
    Cancer Res., 2009;69(15):6249-55.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  13. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Luminex Development
  14. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
    Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
    Clin. Chem., 2007;53(11):2010-2.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  15. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007;109(10):4272-9.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Luminex Development
  16. Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha.
    Authors: Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA
    J. Immunol., 2007;179(7):4840-8.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  17. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007;329(1):214-8.
    Species: Human
    Sample Type: Serum
    Application: Luminex Development
  18. Eotaxin-2 and colorectal cancer: a potential target for immune therapy.
    Authors: Cheadle EJ, Riyad K, Subar D, Rothwell DG, Ashton G, Batha H, Sherlock DJ, Hawkins RE, Gilham DE
    Clin. Cancer Res., 2007;13(19):5719-28.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  19. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
    Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
    AIDS, 2007;21(10):1263-72.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Luminex Development
  20. Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients.
    Authors: Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D
    J. Immunol., 2007;179(6):4272-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Immunodepletion
  21. Combination therapy: Synergistic suppression of virus-induced chemokines in airway epithelial cells.
    Authors: Edwards MR, Johnson MW, Johnston SL
    Am. J. Respir. Cell Mol. Biol., 2006;34(5):616-24.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  22. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006;120(2):220-31.
    Species: Human
    Sample Type: Blister Fluid
    Application: Neut
  23. Enhanced type I interferon signalling promotes Th1-biased inflammation in cutaneous lupus erythematosus.
    Authors: Wenzel J, Worenkamper E, Freutel S, Henze S, Haller O, Bieber T, Tuting T
    J. Pathol., 2005;205(4):435-42.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  24. Simian human immunodeficiency virus-associated pneumonia correlates with increased expression of MCP-1, CXCL10, and viral RNA in the lungs of rhesus macaques.
    Authors: Sui Y, Li S, Pinson D, Adany I, Li Z, Villinger F, Narayan O, Buch S
    Am. J. Pathol., 2005;166(2):355-65.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Neut
  25. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
    Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
    J. Immunol., 2004;173(11):6592-602.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  26. Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
    Authors: Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
    Blood, 2004;104(2):314-20.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  27. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002;169(9):5244-51.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  28. Interleukin-12 induces sustained activation of multiple host inflammatory mediator systems in chimpanzees.
    Authors: Lauw FN, Dekkers PE, te Velde AA, Speelman P, Levi M, Kurimoto M, Hack CE, Van Deventer SJ, van der Poll T
    J. Infect. Dis., 1999;179(3):646-52.
    Species: Primate - Pan troglodytes (Chimpanzee)
    Sample Type: Plasma
    Application: ELISA Development
Expand to show all 28 Citations
Isotype Controls
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Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Average Rating: 4 (Based on 1 review)

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ELISA Human CXCL10/IP-10/CRG-2 Antibody MAB266
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 ELISA Human Anonymous 11/17/2017
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ELISA Human CXCL10/IP-10/CRG-2 Antibody MAB266
ELISA: Human CXCL10/IP-10/CRG-2 Antibody [MAB266]

Summary

ApplicationELISA
Sample TestedSerum and Plasma
SpeciesHuman

Other Experimental Details

Other Experimental DetailsSandwich ELISA using MAB266 on solid phase and BAF266 as detection.

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