Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Feline, Primate - Macaca mulatta (Rhesus Macaque), Primate - Pan troglodytes (Chimpanzee)

Applications

Validated:

Immunohistochemistry, ELISA, Neutralization, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CXCL10/IP-10/CRG-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CXCL10/IP-10/CRG-2
Val22-Pro98
Accession # P02778

Specificity

Detects human CXCL10/IP-10/CRG-2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.20 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CXCL10/IP‑10/CRG‑2 Antibody

CXCL10/IP-10 antibody in Human PBMCs by Immunocytochemistry (ICC).

CXCL10/IP-10 in Human PBMCs.

CXCL10/IP-10 was detected in immersion fixed PHA-treated human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human CXCL10/IP-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-266-NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CXCL10/IP-10/CRG-2 antibody in Human Tonsil by Immunohistochemistry (IHC-P).

CXCL10/IP‑10/CRG‑2 in Human Tonsil.

CXCL10/IP-10/CRG-2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human CXCL10/IP-10/CRG-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-266-NA) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Chemotaxis Induced by CXCL10/IP‑10 and Neutralization by Human CXCL10/IP‑10 Antibody.

Chemotaxis Induced by CXCL10/IP‑10 and Neutralization by Human CXCL10/IP‑10 Antibody.

Recombinant Human CXCL10/IP-10 (Catalog # 266-IP) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL10/IP-10 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL10/IP-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-266-NA). The ND50 is typically 1-4 µg/mL.
Human CXCL10/IP-10/CRG-2 Antibody in ELISA Standard Curve.

Human CXCL10/IP‑10/CRG‑2 ELISA Standard Curve.

Recombinant Human CXCL10/IP-10/CRG-2 protein was serially diluted 2-fold and captured by Mouse Anti-Human CXCL10/IP-10/CRG-2 Monoclonal Antibody (Catalog # MAB2661) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL10/IP-10/CRG-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-266-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Detection of CXCL10/IP-10/CRG-2 by Immunohistochemistry

Detection of CXCL10/IP-10/CRG-2 by Immunohistochemistry

The expression of CXCR3 and CXCL10 in AIH. (A) Multiplex immunofluorescence staining of CXCR3 (red), CD19 (yellow), and TSPAN1 (green) in the liver of AIH patients. (B) Representative confocal staining of TSPAN1 and CXCL10 in the livers of AIH. (C) Immunohistochemical staining of CXCL10 in AIH and HC (×400). Correlation analysis of hepatic CXCL10 expression degree with the numbers of TSPAN1. (D) CD19+ B cells were placed in the upper chambers and complete medium was added in the lower chambers with rhCXCL10 (3 μg/mL) or not for 6 hours. The percentage of TSPAN1+ cells was measured by flow cytometry. Boxes represent the 25th–75th percentile of the distribution; the median is shown as a thick line in the middle of the box; whiskers extend to values with 1.5 times the difference between the 25th and 75th percentiles. *P < 0.05, **P < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36591302), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CXCL10/IP-10/CRG-2 by Immunohistochemistry

Detection of CXCL10/IP-10/CRG-2 by Immunohistochemistry

The expression of CXCR3 and CXCL10 in AIH. (A) Multiplex immunofluorescence staining of CXCR3 (red), CD19 (yellow), and TSPAN1 (green) in the liver of AIH patients. (B) Representative confocal staining of TSPAN1 and CXCL10 in the livers of AIH. (C) Immunohistochemical staining of CXCL10 in AIH and HC (×400). Correlation analysis of hepatic CXCL10 expression degree with the numbers of TSPAN1. (D) CD19+ B cells were placed in the upper chambers and complete medium was added in the lower chambers with rhCXCL10 (3 μg/mL) or not for 6 hours. The percentage of TSPAN1+ cells was measured by flow cytometry. Boxes represent the 25th–75th percentile of the distribution; the median is shown as a thick line in the middle of the box; whiskers extend to values with 1.5 times the difference between the 25th and 75th percentiles. *P < 0.05, **P < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36591302), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CXCL10/IP‑10/CRG‑2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human CXCL10/IP‑10/CRG‑2 Monoclonal Antibody (Catalog # MAB2661).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human CXCL10/IP-10 DuoSet ELISA Kit (Catalog # DY266) for convenient development of a sandwich ELISA or the Human CXCL10/IP-10 Quantikine ELISA Kit (Catalog # DIP100) for a complete optimized ELISA.

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells treated with PHA

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood monocytes treated with Recombinant Human IFN‑ gamma (Catalog # 285‑IF), fixed with paraformaldehyde, and permeabilized with saponin

Neutralization

Measured by its ability to neutralize CXCL10/IP‑10/CRG‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR3. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 0.2 µg/mL Recombinant Human CXCL10/IP‑10/CRG‑2.

Reviewed Applications

Read 3 reviews rated 4.3 using AF-266-NA in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL10/IP-10/CRG-2

CXCL10 was originally identified as an IFN-gamma -inducible gene in monocytes, fibroblasts, and endothelial cells. It has since been shown that CXCL10 mRNA is also induced by LPS, IL-1 beta, TNF-alpha, IL-12, and viruses. Additional cell types that have been shown to express CXCL10 include activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. CXCL10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human CXCL10, CRG-2, has been cloned and shown to share approximately 67% amino acid sequence identity with human CXCL10. Human CXCL10 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted protein. The amino acid sequence of CXCL10 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes. CXCL10 has been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent antitumor effect. A chemokine receptor specific for CXCL10 and MIG has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

References

  1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
  2. Wang, X. et al.(1996) J. Biol. Chem. 271:24286.

Alternate Names

CRG-2, CRG2, IP-10

Entrez Gene IDs

3627 (Human); 15945 (Mouse)

Gene Symbol

CXCL10

UniProt

P02778

Additional CXCL10/IP-10/CRG-2 Products

Product Documents for Human CXCL10/IP‑10/CRG‑2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CXCL10/IP‑10/CRG‑2 Antibody

For research use only

Citations for Human CXCL10/IP‑10/CRG‑2 Antibody

Customer Reviews for Human CXCL10/IP‑10/CRG‑2 Antibody (3)

4.3 out of 5
3 Customer Ratings
5 Stars
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4 Stars
67%
3 Stars
0%
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1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human CXCL10/IP-10/CRG-2 Antibody
    Name: Maximilian Haist
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human Tonsil tissue
    Species: Human
    Verified Customer | Posted 01/18/2023
    CXCL10 stained human tonsil tissue
    Human CXCL10/IP‑10/CRG‑2 Antibody AF-266-NA
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23423575
    Species: Human
    Verified Customer | Posted 01/07/2015
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 24080276
    Species: Human
    Verified Customer | Posted 01/07/2015

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