Human CXCL10/IP-10/CRG-2 PE-conjugated Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CXCL10/IP-10/CRG-2 in ELISAs and Western blots. In Western blots, does not cross-react with recombinant human CXCL1, 2, 3, 5, 8, 9, 12/SDF-1 beta, recombinant mouse CXCL1, or 2.
  • Source
    Monoclonal Mouse IgG1 Clone # 33036
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human CXCL10/IP-10/CRG-2
    Val22-Pro98
    Accession # P02778.2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of CXCL10/IP‑10/CRG‑2 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS were stained with Mouse Anti-Human CXCL10/IP‑10/CRG‑2 PE‑conjugated Monoclonal Antibody (Catalog # IC266P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CXCL10/IP-10/CRG-2

CXCL10 was originally identified as an IFN-gamma -inducible gene in monocytes, fibroblasts and endothelial cells. It has since been shown that CXCL10 mRNA is also induced by LPS, IL-1 beta, TNF-alpha, IL-12, and viruses. Additional cell types that have been shown to express CXCL10 include activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. CXCL10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human CXCL10, CRG-2, has been cloned and shown to share approximately 67% amino acid sequence identity with human CXCL10. Human CXCL10 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted protein. The amino acid sequence of CXCL10 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes. CXCL10 has been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent antitumor effect. A chemokine receptor specific for CXCL10 and Mig has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

  • References:
    1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
    2. Wang, X. et al. (1996) J. Biol. Chem. 271:24286.
  • Entrez Gene IDs:
    3627 (Human); 15945 (Mouse)
  • Alternate Names:
    C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. High-content cytometry and transcriptomic biomarker profiling of human B-cell activation.
    Authors: Hennig C, Ilginus C, Boztug K, Skokowa J, Marodi L, Szaflarska A, Sass M, Pignata C, Kilic S, Caragol I, Baumann U, Klein C, Welte K, Hansen G
    J Allergy Clin Immunol, 2014;133(1):172-80.e1-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Chip Cytometry
  2. Functional OCT4-specific CD4 and CD8 T cells in healthy controls and ovarian cancer patients.
    Authors: Di J, Massuger L, Duiveman-de Boer T, Zusterzeel P, Figdor C, Torensma R
    Oncoimmunology, 2013;2(5):e24271.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Interleukin-27 induces a STAT1/3- and NF-kappaB-dependent proinflammatory cytokine profile in human monocytes.
    Authors: Guzzo C, Che Mat NF, Gee K
    J. Biol. Chem., 2010;285(32):24404-11.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Flow-cytometric analysis of cytokine production in human paracoccidioidomycosis.
    Authors: Mamoni RL, Blotta MH
    Cytokine, 2006;35(3):207-16.
    Species: Human
    Sample Type: Whole Blood
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 38  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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