Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 422208
View all CXCL17/VCC-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CXCL17/VCC‑1
Leu24-Leu119
Accession # Q6UXB2

Specificity

Detects human CXCL17/VCC‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse CXCL17 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CXCL17/VCC‑1 Antibody

Detection of CXCL17/VCC-1 antibody in A549 Human Cell Line antibody by Flow Cytometry.

Detection of CXCL17/VCC-1 in A549 Human Cell Line by Flow Cytometry.

A549 human lung carcinoma cell line was stained with Mouse Anti-Human CXCL17/VCC-1 Monoclonal Antibody (Catalog # MAB4207, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
CXCL17/VCC-1 antibody in A549 Human Cell Line by Immunocytochemistry (ICC).

CXCL17/VCC‑1 in A549 Human Cell Line.

CXCL17/VCC-1 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human CXCL17/VCC-1 Monoclonal Antibody (Catalog # MAB4207) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of CXCL17/VCC-1 by Immunohistochemistry

Detection of CXCL17/VCC-1 by Immunohistochemistry

CXCL17 expression in situ in HCC tumors.(A) Representative sites depicting CXCL17-producing cells stained brown in human chronic hepatitis liver, nontumor, peritumoral stroma, and intratumoral regions in HCC. Representative sites with low (upper panels) and high (lower panels) magnification were shown. Black arrows indicated CXCL17+ cells. (B) Multiple staining of MPO (green), CXCL17 (red), and DAPI (blue, nuclei) in paraffin-embedded sections analyzed by confocal microscopy. The coexistence of MPO and CXCL17 confirmed that a proportion of MPO+ neutrophils expressed CXCL17. White arrows indicated representative neutrophils expressed CXCL17. (C) Proportions of CXCL17+MPO+ cells in CXCL17+ cells or MPO+ cells of HCC tissue. Results are expressed as mean ± SEM (bars). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25303284), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CXCL17/VCC-1 by Western Blot

Detection of CXCL17/VCC-1 by Western Blot

Overexpression of CXCL17 upregulated CCL20 expression. Eighty-nine DEGs (a) shared by CXCL17 and GPR35 were used for PPI analysis (b). CXCL17 mRNA level in the CCLE cell lines (c). The protein level of CCL20 was significantly upregulated after transfecting a CXCL17 overexpression plasmid into the HGC27 cells (d). The mRNA level of SFRP2 (e) was downregulated upon the overexpression of CXLC17, while the CDX1 level (f) remained unchanged. ** p < 0.01. ns, no significance; PPI, protein–protein interaction; CCLE, Cancer Cell Line Encyclopedia. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36614059), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CXCL17/VCC-1 by Immunohistochemistry

Detection of CXCL17/VCC-1 by Immunohistochemistry

The protein expression of CXCL17 (a) and GPR35 (b) in different pathological lesions determined by immunohistochemistry. ** p < 0.01, *** p < 0.001. (c) Representative immunostaining images of CXCL17 and GPR35 in different gastric pathological lesions. Magnification: ×200. ns, no significance; NAG-NOR, normal gastric gland of non-atrophic gastritis; AG-IM, intestinal metaplasia of atrophic gastritis, GC-IM, intestinal metaplasia adjacent to gastric cancer; GC, gastric cancer. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36614059), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CXCL17/VCC-1 by Immunohistochemistry

Detection of CXCL17/VCC-1 by Immunohistochemistry

The protein expression of CXCL17 (a) and GPR35 (b) in different pathological lesions determined by immunohistochemistry. ** p < 0.01, *** p < 0.001. (c) Representative immunostaining images of CXCL17 and GPR35 in different gastric pathological lesions. Magnification: ×200. ns, no significance; NAG-NOR, normal gastric gland of non-atrophic gastritis; AG-IM, intestinal metaplasia of atrophic gastritis, GC-IM, intestinal metaplasia adjacent to gastric cancer; GC, gastric cancer. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36614059), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CXCL17/VCC-1 by Western Blot

Detection of CXCL17/VCC-1 by Western Blot

Overexpression of CXCL17 upregulated CCL20 expression. Eighty-nine DEGs (a) shared by CXCL17 and GPR35 were used for PPI analysis (b). CXCL17 mRNA level in the CCLE cell lines (c). The protein level of CCL20 was significantly upregulated after transfecting a CXCL17 overexpression plasmid into the HGC27 cells (d). The mRNA level of SFRP2 (e) was downregulated upon the overexpression of CXLC17, while the CDX1 level (f) remained unchanged. ** p < 0.01. ns, no significance; PPI, protein–protein interaction; CCLE, Cancer Cell Line Encyclopedia. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36614059), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CXCL17/VCC‑1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: A549 human lung carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Human CXCL17 (Catalog # 4207-DM)

Reviewed Applications

Read 1 review rated 5 using MAB4207 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL17/VCC-1

CXCL17, also known as dendritic cell and monocyte chemokine-like protein (DMC) and VEGF-correlated chemokine-1 (VCC-1), is a secreted molecule with a size and predicted three-dimensional folding pattern similar to that of chemokines CXCL8/IL-8 and CXCL14/BRAK (1, 2). It has no predicted N-glycosylation site. Cleavage of a 23 amino acid (aa) signal sequence yields the mature 96 aa human CXCL17. CXCL17 is constitutively produced by airway and intestinal epithelium (1). It induces the chemotaxis of quiescent, but not LPS-activated peripheral blood monocytes and dendritic cells (1). CXCL17 expression is increased in endothelial cells when they are induced to form tubes in vitro (2). Transgenic overexpression in NIH3T3 cells causes upregulation of proteins such as VEGF and FGF basic, and increases cell growth rate and tumorigenicity (2). CXCL17, plus two other chemokines that play roles in angiogenesis, CXCL1/GRO and CXCL8/IL-8, show a correlated expression pattern with VEGF in primary lung, breast and esophageal tumors (2). CXCL17 is, therefore, suggested to play a role in tumor angiogenesis. Mature human CXCL17 shares 73%, 71% and 64% amino acid sequence identity with bovine, mouse and rat CXCL17, respectively.

References

  1. Pisabarro, M.T. et al. (2006) J. Immunol. 176:2069.
  2. Weinstein, E.J. et al. (2006) Biochem. Biophys. Res. Commun. 350:74.

Alternate Names

CXCL17, DMC, VCC-1, VCC1

Entrez Gene IDs

284340 (Human); 232983 (Mouse)

Gene Symbol

CXCL17

UniProt

Q6UXB2

Additional CXCL17/VCC-1 Products

Product Documents for Human CXCL17/VCC‑1 Antibody

Certificate of Analysis

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Product Specific Notices for Human CXCL17/VCC‑1 Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Human CXCL17/VCC‑1 Antibody

Customer Reviews for Human CXCL17/VCC‑1 Antibody (1)

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    Application: Immunohistochemistry
    Sample Tested: Skin tissue
    Species: Human
    Verified Customer | Posted 02/24/2020
    Human CXCL17/VCC‑1 Antibody MAB4207

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