Human CXCL17/VCC-1 Antibody

Catalog # Availability Size / Price Qty
Detection of CXCL17/VCC-1 in A549 Human Cell Line by Flow Cytometry.
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Product Details
Citations (4)
Supplemental Products
Reviews (1)

Human CXCL17/VCC-1 Antibody Summary

Species Reactivity
Detects human CXCL17/VCC‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse CXCL17 is observed.
Monoclonal Mouse IgG2B Clone # 422208
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human CXCL17/VCC‑1
Accession # Q6UXB2
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human CXCL17 (Catalog # 4207-DM)
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of CXCL17/VCC-1 antibody in A549 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of CXCL17/VCC-1 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Mouse Anti-Human CXCL17/VCC-1 Monoclonal Antibody (Catalog # MAB4207, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Immunocytochemistry CXCL17/VCC-1 antibody in A549 Human Cell Line by Immunocytochemistry (ICC). View Larger

CXCL17/VCC‑1 in A549 Human Cell Line. CXCL17/VCC-1 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human CXCL17/VCC-1 Monoclonal Antibody (Catalog # MAB4207) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL17/VCC-1

CXCL17, also known as dendritic cell and monocyte chemokine-like protein (DMC) and VEGF-correlated chemokine-1 (VCC-1), is a secreted molecule with a size and predicted three-dimensional folding pattern similar to that of chemokines CXCL8/IL-8 and CXCL14/BRAK (1, 2). It has no predicted N-glycosylation site. Cleavage of a 23 amino acid (aa) signal sequence yields the mature 96 aa human CXCL17. CXCL17 is constitutively produced by airway and intestinal epithelium (1). It induces the chemotaxis of quiescent, but not LPS-activated peripheral blood monocytes and dendritic cells (1). CXCL17 expression is increased in endothelial cells when they are induced to form tubes in vitro (2). Transgenic overexpression in NIH3T3 cells causes upregulation of proteins such as VEGF and FGF basic, and increases cell growth rate and tumorigenicity (2). CXCL17, plus two other chemokines that play roles in angiogenesis, CXCL1/GRO and CXCL8/IL-8, show a correlated expression pattern with VEGF in primary lung, breast and esophageal tumors (2). CXCL17 is, therefore, suggested to play a role in tumor angiogenesis. Mature human CXCL17 shares 73%, 71% and 64% amino acid sequence identity with bovine, mouse and rat CXCL17, respectively.

  1. Pisabarro, M.T. et al. (2006) J. Immunol. 176:2069.
  2. Weinstein, E.J. et al. (2006) Biochem. Biophys. Res. Commun. 350:74.
Entrez Gene IDs
284340 (Human); 232983 (Mouse)
Alternate Names
Chemokine (C-X-C Motif) Ligand 17; C-X-C Motif Chemokine 17; CXCL17; Dcip1; Dendritic Cell And Monocyte Chemokine-Like Protein; DMC; UNQ473; VCC1; VCC-1; VEGF Coregulated Chemokine 1; VEGF Co-Regulated Chemokine 1

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Citations for Human CXCL17/VCC-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Involvement of CXCL17 and GPR35 in Gastric Cancer Initiation and Progression
    Authors: Y Li, A Liu, S Liu, L Yan, Y Yuan, Q Xu
    International Journal of Molecular Sciences, 2022;24(1):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Prognostic value of CXCL17 and CXCR8 expression in patients with colon cancer
    Authors: H Yao, Y Lv, X Bai, Z Yu, X Liu
    Oncol Lett, 2020;20(3):2711-2720.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. MiR-4513 mediates the proliferation and apoptosis of oral squamous cell carcinoma cells via targeting CXCL17
    Authors: YX Xu, J Sun, WL Xiao, YS Liu, J Yue, LF Xue, J Deng, KQ Zhi, YL Wang
    Eur Rev Med Pharmacol Sci, 2019;23(9):3821-3828.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. CXCL17 is a major chemotactic factor for lung macrophages.
    Authors: Burkhardt A, Maravillas-Montero J, Carnevale C, Vilches-Cisneros N, Flores J, Hevezi P, Zlotnik A
    J Immunol, 2014;193(3):1468-74.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P


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Human CXCL17/VCC-1 Antibody
By Anonymous on 02/24/2020
Application: IHC Sample Tested: Skin tissue Species: Human