Human CXCR5 Alexa Fluor® 488-conjugated Antibody

Clone 51505 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB190G-100UG
Detection of CXCR5 in Human Peripheral Blood Cells by Flow Cytometry.
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Human CXCR5 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human CXCR5 transfectants but not the parental cell lines in flow cytometry. Does not cross-react with human CXCR2, CXCR3, or CXCR4 transfectants.
Source
Monoclonal Mouse IgG2B Clone # 51505
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CXCR5
Met1-Phe372
Accession # P32302
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CXCR5 in Human Peripheral Blood Cells by Flow Cytometry. View Larger

Detection of CXCR5 in Human Peripheral Blood Cells by Flow Cytometry. Human peripheral blood cells were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CXCR5 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB190G) or (B) Mouse IgG2B Alexa Fluor 488 Isotype Control (Catalog # IC0041G). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR5

CXCR5, also known as BLR-1, is a 7 transmembrane domain protein expressed on B cells. CXCR5 mediates B cell migration following binding of CXCL13.

Entrez Gene IDs
643 (Human); 12145 (Mouse); 29363 (Rat)
Alternate Names
BLR1; BLR1MDR-15; Burkitt lymphoma receptor 1; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif)receptor 5); Burkitt lymphoma receptor 1, GTP-binding protein; CD185 antigen; CD185; chemokine (C-X-C motif) receptor 5; C-X-C chemokine receptor type 5; CXCR5; CXC-R5; MDR15CXCR-5; MGC117347; Monocyte-derived receptor 15

Product Datasheets

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