Human CXCR5 Fluorescein-conjugated Antibody

Clone 51505 was used by HLDA to establish CD designation.
(4 citations)   
  • Species Reactivity
  • Specificity
    Stains human CXCR5 transfectants but not the parental cell lines in flow cytometry. Does not cross-react with human CXCR2, CXCR3, or CXCR4 transfectants.
  • Source
    Monoclonal Mouse IgG2B Clone # 51505
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human CXCR5
    Accession # P32302
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CXCR5 in Human Peripheral Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CXCR5 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB190F) or (B) Mouse IgG2B Fluorescein Isotype Control (Catalog # IC0041F). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CXCR5
CXCR5, also known as BLR-1, is a 7 transmembrane domain protein expressed on B cells. CXCR5 mediates B cell migration following binding of CXCL13.
  • Entrez Gene IDs:
    643 (Human); 12145 (Mouse); 29363 (Rat)
  • Alternate Names:
    BLR1; BLR1MDR-15; Burkitt lymphoma receptor 1; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif)receptor 5); Burkitt lymphoma receptor 1, GTP-binding protein; CD185 antigen; CD185; chemokine (C-X-C motif) receptor 5; C-X-C chemokine receptor type 5; CXCR5; CXC-R5; MDR15CXCR-5; MGC117347; Monocyte-derived receptor 15
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. CD4+ T cell-derived IL-21 and deprivation of CD40 signaling favor the in vivo development of granzyme B-expressing regulatory B cells in HIV patients.
    Authors: Kaltenmeier C, Gawanbacht A, Beyer T, Lindner S, Trzaska T, van der Merwe J, Harter G, Gruner B, Fabricius D, Lotfi R, Schwarz K, Schutz C, Honig M, Schulz A, Kern P, Bommer M, Schrezenmeier H, Kirchhoff F, Jahrsdorfer B
    J Immunol, 2015;194(8):3768-77.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. CXCR5(+) T helper cells mediate protective immunity against tuberculosis.
    Authors: Slight S, Rangel-Moreno J, Gopal R, Lin Y, Fallert Junecko B, Mehra S, Selman M, Becerril-Villanueva E, Baquera-Heredia J, Pavon L, Kaushal D, Reinhart T, Randall T, Khader S
    J Clin Invest, 2013;123(2):712-26.
    Species: Human
    Sample Type: tissue
    Application: IHC Paraffin-embedded
  3. CCR2 identifies a stable population of human effector memory CD4+ T cells equipped for rapid recall response.
    Authors: Zhang HH, Song K, Rabin RL, Hill BJ, Perfetto SP, Roederer M, Douek DC, Siegel RM, Farber JM
    J. Immunol., 2010;185(11):6646-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients.
    Authors: Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D
    J. Immunol., 2007;179(6):4272-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Fluorescein‑conjugated Control Antibody

Ctrl IC0041F 6  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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