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Human CXCR5 PE-conjugated Antibody

Catalog #: FAB190P
25 Citations 3 Reviews Datasheet / COA / SDS
Clone 51505 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB190P-025
FAB190P-100

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Detection of CXCR5 in CD4+ Human PBMCs by Flow Cytometry.
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Product Details
Citations (25)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CXCR5 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human CXCR5 transfectants but not the parental cell lines in flow cytometry. Does not cross-react with human CXCR2, CXCR3, or CXCR4 transfectants.
Source
Monoclonal Mouse IgG2B Clone # 51505
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CXCR5
Met1-Phe372
Accession # P32302
Formulation
Supplied in a saline solution containing BSA and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CXCR5 antibody in CD4+ Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CXCR5 in CD4+ Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CXCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB190P) or (B) Mouse IgG2B isotype control antibody (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CXCR5 antibody in CD19+ Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CXCR5 in CD19+ Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Mouse Anti-Human CXCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB190P) or (B) Mouse IgG2B control antibody (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR5

CXCR5, also known as BLR-1, is a 7 transmembrane domain protein expressed on B cells. CXCR5 mediates B cell migration following binding of CXCL13.

Entrez Gene IDs
643 (Human); 12145 (Mouse); 29363 (Rat)
Alternate Names
BLR1; BLR1MDR-15; Burkitt lymphoma receptor 1; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif)receptor 5); Burkitt lymphoma receptor 1, GTP-binding protein; CD185 antigen; CD185; chemokine (C-X-C motif) receptor 5; C-X-C chemokine receptor type 5; CXCR5; CXC-R5; MDR15CXCR-5; MGC117347; Monocyte-derived receptor 15

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Citations for Human CXCR5 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. A distinct plasmablast and na�ve B-cell phenotype in primary immune thrombocytopenia
    Haematologica, 2016-03-11;101(6):698-706.
  2. CXCR5 CAR-T cells simultaneously target B cell non-Hodgkin's lymphoma and tumor-supportive follicular T helper cells
    Authors: M Bunse, J Pfeilschif, J Bluhm, M Zschummel, JJ Joedicke, A Wirges, H Stark, V Kretschmer, M Chmielewsk, W Uckert, H Abken, J Westermann, A Rehm, UE Höpken
    Nature Communications, 2021-01-11;12(1):240.
    Species: Human, Xenograft
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  3. Follicular T Cells from smB(-) Common Variable Immunodeficiency Patients Are Skewed Toward a Th1 Phenotype
    Authors: V Cunill, A Clemente, N Lanio, C Barceló, V Andreu, J Pons, JM Ferrer
    Front Immunol, 2017-02-27;8(0):174.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. The serum CXCL13 level is associated with the Glasgow Prognostic Score in extranodal NK/T-cell lymphoma patients
    Authors: Seok Jin Kim, Kyung Ju Ryu, Mineui Hong, Young Hyeh Ko, Won Seog Kim
    Journal of Hematology & Oncology
  5. NCR(+)ILC3 concentrate in human lung cancer and associate with intratumoral lymphoid structures
    Authors: Paolo Carrega, Fabrizio Loiacono, Emma Di Carlo, Angelo Scaramuccia, Marco Mora, Romana Conte et al.
    Nature Communications
  6. Mesenchymal stromal cell secretome up-regulates 47 kDa CXCR4 expression, and induce invasiveness in neuroblastoma cell lines.
    Authors: Shankar V, Hori H, Kihira K, Lei Q, Toyoda H, Iwamoto S, Komada Y
    PLoS ONE, 2015-03-16;10(3):e0120069.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Herpes simplex virus enhances chemokine function through modulation of receptor trafficking and oligomerization.
    Authors: Martinez-Martin N, Viejo-Borbolla A, Martin R, Blanco S, Benovic J, Thelen M, Alcami A
    Nat Commun, 2015-01-27;6(0):6163.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
    Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
    J Immunol, 2014-02-19;192(6):2634-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. IL-12 directs further maturation of ex vivo differentiated NK cells with improved therapeutic potential.
    Authors: Lehmann, Dorit, Spanholtz, Jan, Sturtzel, Caterina, Tordoir, Marleen, Schlechta, Bernhard, Groenewegen, Dirk, Hofer, Erhard
    PLoS ONE, 2014-01-31;9(1):e87131.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis.
    Authors: Badr G, Lefevre EA, Mohany M
    PLoS ONE, 2011-09-01;6(9):e23741.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Primary B-cell deficiencies reveal a link between human IL-17-producing CD4 T-cell homeostasis and B-cell differentiation.
    Authors: Barbosa RR, Silva SP, Silva SL, Melo AC, Pedro E, Barbosa MP, Pereira-Santos MC, Victorino RM, Sousa AE
    PLoS ONE, 2011-08-03;6(8):e22848.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Increased numbers of immature plasma cells in peripheral blood specifically overexpress chemokine receptor CXCR3 and CXCR4 in patients with ulcerative colitis.
    Authors: Hosomi S, Oshitani N, Kamata N, Sogawa M, Okazaki H, Tanigawa T, Yamagami H, Watanabe K, Tominaga K, Watanabe T, Fujiwara Y, Maeda K, Hirakawa K, Arakawa T
    Clin. Exp. Immunol., 2010-11-19;163(2):215-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010-02-17;184(6):2886-98.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Changes in chemokines and chemokine receptor expression on tonsillar B cells upon Epstein-Barr virus infection.
    Authors: Ehlin-Henriksson B, Liang W, Cagigi A, Mowafi F, Klein G, Nilsson A
    Immunology, 2009-08-01;127(4):549-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Chemokine CXCL13 is overexpressed in the tumour tissue and in the peripheral blood of breast cancer patients.
    Authors: Panse J, Friedrichs K, Marx A, Hildebrandt Y, Luetkens T, Bartels K, Horn C, Stahl T, Cao Y, Milde-Langosch K, Niendorf A, Kroger N, Wenzel S, Leuwer R, Bokemeyer C, Hegewisch-Becker S, Atanackovic D
    Br. J. Cancer, 2008-09-16;99(6):930-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Altered expression of the receptor-ligand pair CXCR5/CXCL13 in B cells during chronic HIV-1 infection.
    Authors: Cagigi A, Mowafi F, Phuong Dang LV, Tenner-Racz K, Atlas A, Grutzmeier S, Racz P, Chiodi F, Nilsson A
    Blood, 2008-09-09;112(12):4401-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Evidence for HIV-associated B cell exhaustion in a dysfunctional memory B cell compartment in HIV-infected viremic individuals.
    Authors: Moir S, Ho J, Malaspina A, Wang W, DiPoto AC, O'Shea MA, Roby G, Kottilil S, Arthos J, Proschan MA, Chun TW, Fauci AS
    J. Exp. Med., 2008-07-14;205(8):1797-805.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation.
    Authors: Maru SV, Holloway KA, Flynn G, Lancashire CL, Loughlin AJ, Male DK, Romero IA
    J. Neuroimmunol., 2008-06-09;199(1):35-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. CD43, but not P-selectin glycoprotein ligand-1, functions as an E-selectin counter-receptor in human pre-B-cell leukemia NALL-1.
    Authors: Nonomura C, Kikuchi J, Kiyokawa N, Ozaki H, Mitsunaga K, Ando H, Kanamori A, Kannagi R, Fujimoto J, Muroi K, Furukawa Y, Nakamura M
    Cancer Res., 2008-02-01;68(3):790-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. BAFF enhances chemotaxis of primary human B cells: a particular synergy between BAFF and CXCL13 on memory B cells.
    Authors: Badr G, Borhis G, Lefevre EA, Chaoul N, Deshayes F, Dessirier V, Lapree G, Tsapis A, Richard Y
    Blood, 2008-01-02;111(5):2744-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Overexpression of the CXCR5 chemokine receptor, and its ligand, CXCL13 in B-cell chronic lymphocytic leukemia.
    Authors: Burkle A, Niedermeier M, Schmitt-Graff A, Wierda WG, Keating MJ, Burger JA
    Blood, 2007-07-25;110(9):3316-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells.
    Authors: Ettinger R, Sims GP, Robbins R, Withers D, Fischer RT, Grammer AC, Kuchen S, Lipsky PE
    J. Immunol., 2007-03-01;178(5):2872-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Chemokine receptors in head and neck cancer: association with metastatic spread and regulation during chemotherapy.
    Authors: Muller A, Sonkoly E, Eulert C, Gerber PA, Kubitza R, Schirlau K, Franken-Kunkel P, Poremba C, Snyderman C, Klotz LO, Ruzicka T, Bier H, Zlotnik A, Whiteside TL, Homey B, Hoffmann TK
    Int. J. Cancer, 2006-05-01;118(9):2147-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Chemokine receptor expression in EBV-associated lymphoproliferation in hu/SCID mice: implications for CXCL12/CXCR4 axis in lymphoma generation.
    Authors: Piovan E, Tosello V, Indraccolo S, Cabrelle A, Baesso I, Trentin L, Zamarchi R, Tamamura H, Fujii N, Semenzato G, Chieco-Bianchi L, Amadori A
    Blood, 2004-09-28;105(3):931-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
    Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
    J. Leukoc. Biol., 2003-05-01;73(5):584-90.
    Species: Human
    Sample Types: CSF
    Applications: Flow Cytometry

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Reviews for Human CXCR5 PE-conjugated Antibody

Average Rating: 4.7 (Based on 3 Reviews)

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Human CXCR5 PE-conjugated Antibody
By Chi P on 04/25/2018
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human

The PBMCs were transduced with CXCR5 resulting in a high population expressing CXCR5 PE

Flow Cytometry Human CXCR5 PE-conjugated Antibody FAB190P
Human CXCR5 PE-conjugated Antibody
By Anonymous on 03/21/2018
Application: Flow Sample Tested: Blood mononuclear cells (PBMCs) Species: Rhesus Macaque
Human CXCR5 PE‑conjugated Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: Peripheral blood cells Species: Human

Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. Lineage Cocktail 1 (BD Biosciences) comprises CD3, CD14, CD16, CD19, CD20, and CD56 antibodies.
Specificity: Specific
Sensitivity: Sensitive
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100

Flow Cytometry Human CXCR5 PE‑conjugated Antibody FAB190P