Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Xenograft

Applications

Validated:

Flow Cytometry

Cited:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 51505
View all CXCR5 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CXCR5
Met1-Phe372
Accession # P32302

Specificity

Stains human CXCR5 transfectants but not the parental cell lines in flow cytometry. Does not cross-react with human CXCR2, CXCR3, or CXCR4 transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CXCR5 PE‑conjugated Antibody

Detection of CXCR5 antibody in CD4+ Human PBMCs antibody by Flow Cytometry.

Detection of CXCR5 in CD4+ Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CXCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB190P) or (B) Mouse IgG2B isotype control antibody (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
Detection of CXCR5 antibody in CD19+ Human PBMCs antibody by Flow Cytometry.

Detection of CXCR5 in CD19+ Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Mouse Anti-Human CXCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB190P) or (B) Mouse IgG2B control antibody (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CXCR5 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Reviewed Applications

Read 5 reviews rated 4.4 using FAB190P in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

For liquid material, refer to CoA for concentration.

Formulation

Supplied in a saline solution containing BSA and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCR5

CXCR5, also known as BLR-1, is a 7 transmembrane domain protein expressed on B cells. CXCR5 mediates B cell migration following binding of CXCL13.

Alternate Names

BLR1, CD185, CXCR5

Entrez Gene IDs

643 (Human); 12145 (Mouse); 29363 (Rat)

Gene Symbol

CXCR5

UniProt

P32302

Additional CXCR5 Products

Product Documents for Human CXCR5 PE‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CXCR5 PE‑conjugated Antibody

For research use only

Citations for Human CXCR5 PE‑conjugated Antibody

Customer Reviews for Human CXCR5 PE‑conjugated Antibody (5)

4.4 out of 5
5 Customer Ratings
5 Stars
40%
4 Stars
60%
3 Stars
0%
2 Stars
0%
1 Stars
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Customer Images

Showing  1 - 5 of 5 reviews Showing All
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  • Human CXCR5 PE-conjugated Antibody
    Name: Chi P
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 04/25/2018
    The PBMCs were transduced with CXCR5 resulting in a high population expressing CXCR5 PE
    Human CXCR5 PE‑conjugated Antibody FAB190P
  • Human CXCR5 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Blood mononuclear cells (PBMCs)
    Species: Rhesus Macaque
    Verified Customer | Posted 03/21/2018
  • Human CXCR5 PE‑conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood cells
    Species: Human
    Verified Customer | Posted 10/26/2015
    Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. Lineage Cocktail 1 (BD Biosciences) comprises CD3, CD14, CD16, CD19, CD20, and CD56 antibodies. <br />Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: 2% Human Serum, 0.5 mM EDTA in PBS<br />Dilution: 1/100
    Human CXCR5 PE‑conjugated Antibody FAB190P
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 22367177
    Species: Human
    Verified Customer | Posted 01/21/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 22367177
    Species: Human
    Verified Customer | Posted 01/21/2015

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