Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Human CXCR5 Antibody.|
Recombinant Human CXCL13/BLC/BCA‑1 (Catalog # 801-CX) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR5 in a dose‑dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL13/BLC/BCA‑1 (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190). The ND50 is typically
|CXCR5 in Human PBMCs. CXCR5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 637-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL008) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.|
|CXCR5 in Human Kidney. CXCR5 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
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