Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Rat, Rabbit

Applications

Validated:

Immunohistochemistry, Neutralization, Flow Cytometry, Immunocytochemistry, CyTOF-reported

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Biacore Binding Assay, Bioassay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 51505
View all CXCR5 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CXCR5
Met1-Phe372
Accession # P32302

Specificity

Stains human CXCR5 transfectants but not the parental cell lines in flow cytometry. Does not cross-react with human CXCR2, CXCR3, or CXCR4 transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CXCR5 Antibody

Detection of CXCR5 antibody in CD19+ Human PBMCs antibody by Flow Cytometry.

Detection of CXCR5 in CD19+ Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) or (B) Mouse IgG2B control antibody (Catalog # MAB0041) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
CXCR5 antibody in Human PBMCs by Immunocytochemistry (ICC).

CXCR5 in Human PBMCs.

CXCR5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 637-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL008) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CXCR5 antibody in Human Kidney by Immunohistochemistry (IHC-P).

CXCR5 in Human Kidney.

CXCR5 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Human CXCR5 Antibody.

Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Human CXCR5 Antibody.

Recombinant Human CXCL13/BLC/BCA-1 (Catalog # 801-CX) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL13/BLC/BCA-1 (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190). The ND50 is typically 0.25-1.5 µg/mL.
Detection of Human CXCR5 by Western Blot

Detection of Human CXCR5 by Western Blot

Inverse correlation between p53 and CXCR5 expression and function in MCF-7 cells.(A) p53 knock-down results in an increase in the relative abundance of CXCR5 mRNA. The result of 10 experiments is shown. (B) Changes in p53 and CXCR5 protein levels correlate with the levels of corresponding mRNAs. mRNA levels were measured by RT-PCR in real time. Representative data are shown, the experiment was repeated 2 times for p53 and 3 times for CXCR5. Complete western blots are shown in Supplementary Figure S1. (C) CXCR5 stimulates of c-Jun mRNA expression in MCF-7 cells in p53-dependent manner. Cells were exposed to recombinant CXCL13 for 6 hours prior to RNA isolation. The result of three experiments is shown. *P < 0,01 versus MCF-7. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25786345), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CXCR5 Antibody

Application
Recommended Usage

CyTOF-reported

Ferrell, P.B., Jr. et al. (2016) PLoS ONE 11: e0153207. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood CD19+ B cells

Immunocytochemistry

8-25 µg/mL
Sample:  Immersion fixed human peripheral blood mononuclear cells (PBMCs)

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymph node and human kidney

Neutralization

Measured by its ability to neutralize CXCL13/BLC/BCA‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR5. The Neutralization Dose (ND50) is typically 0.25-1.5 µg/mL in the presence of 0.05 µg/mL Recombinant Human CXCL13/BLC/BCA‑1.

Reviewed Applications

Read 3 reviews rated 4.7 using MAB190 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCR5

CXCR5, also known as BLR-1, is a 7 transmembrane domain protein expressed on B cells. CXCR5 mediates B cell migration following binding of CXCL13.

Alternate Names

BLR1, CD185, CXCR5

Entrez Gene IDs

643 (Human); 12145 (Mouse); 29363 (Rat)

Gene Symbol

CXCR5

UniProt

P32302

Additional CXCR5 Products

Product Documents for Human CXCR5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CXCR5 Antibody

For research use only

Citations for Human CXCR5 Antibody

Customer Reviews for Human CXCR5 Antibody (3)

4.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
33%
3 Stars
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1 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human CXCR5 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Human retinal pigment epithelium cells
    Species: Human
    Verified Customer | Posted 08/19/2021
    Human CXCR5 Antibody MAB190
  • Human CXCR5 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Tonsil tissue
    Species: Human
    Verified Customer | Posted 09/11/2020
    retrieval in high pH buffer Ab was diluted 1:2000(0.25ug/mL)
    Human CXCR5 Antibody MAB190
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 22330139
    Species: Mouse
    Verified Customer | Posted 02/12/2015

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Protocols

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