Human CXCR5 Antibody

Clone 51505 was used by HLDA to establish CD designation.
(20 citations)   
  • Species Reactivity
    Human
  • Specificity
    Stains human CXCR5 transfectants but not the parental cell lines in flow cytometry. Does not cross-react with human CXCR2, CXCR3, or CXCR4 transfectants.
  • Source
    Monoclonal Mouse IgG2B Clone # 51505
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human CXCR5
    Met1-Phe372
    Accession # P32302
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    Human peripheral blood CD19+ B cells
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-reported
    Ferrell, P.B., Jr. et al. (2016) PLoS ONE 11: e0153207. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize CXCL13/BLC/BCA‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR5. The Neutralization Dose (ND50) is typically 0.25-1.5 µg/mL in the presence of 0.05 µg/mL Recombinant Human CXCL13/BLC/BCA‑1.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Human CXCR5 Antibody.
Recombinant Human CXCL13/BLC/BCA‑1 (Catalog # 801-CX) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR5 in a dose‑dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL13/BLC/BCA‑1 (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190). The ND50 is typically
0.25-1.5 µg/mL.
Immunocytochemistry
CXCR5 in Human PBMCs. CXCR5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 637-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL008) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunohistochemistry
CXCR5 in Human Kidney. CXCR5 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCR5
CXCR5, also known as BLR-1, is a 7 transmembrane domain protein expressed on B cells. CXCR5 mediates B cell migration following binding of CXCL13.
  • Entrez Gene IDs:
    643 (Human); 12145 (Mouse); 29363 (Rat)
  • Alternate Names:
    BLR1; BLR1MDR-15; Burkitt lymphoma receptor 1; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif)receptor 5); Burkitt lymphoma receptor 1, GTP-binding protein; CD185 antigen; CD185; chemokine (C-X-C motif) receptor 5; C-X-C chemokine receptor type 5; CXCR5; CXC-R5; MDR15CXCR-5; MGC117347; Monocyte-derived receptor 15
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. p53-dependent expression of CXCR5 chemokine receptor in MCF-7 breast cancer cells.
    Authors: Mitkin N, Hook C, Schwartz A, Biswas S, Kochetkov D, Muratova A, Afanasyeva M, Kravchenko J, Bhattacharyya A, Kuprash D
    Sci Rep, 2015;5(0):9330.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Capture-stabilize approach for membrane protein SPR assays.
    Authors: Chu, Ruiyin, Reczek, David, Brondyk, William
    Sci Rep, 2014;4(0):7360.
    Species: Human
    Sample Type: Peptide
    Application: Biacore
  3. CXCR7 influences the migration of B cells during maturation.
    Authors: Humpert M, Pinto D, Jarrossay D, Thelen M
    Eur J Immunol, 2014;44(3):694-705.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. The MEC1 and MEC2 lines represent two CLL subclones in different stages of progression towards prolymphocytic leukemia.
    Authors: Rasul E, Salamon D, Nagy N, Leveau B, Banati F, Szenthe K, Koroknai A, Minarovits J, Klein G, Klein E
    PLoS ONE, 2014;9(8):e106008.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. IL-15 positively regulates IL-21 production in celiac disease mucosa.
    Authors: Sarra M, Cupi M, Monteleone I, Franze E, Ronchetti G, Di Sabatino A, Gentileschi P, Franceschilli L, Sileri P, Sica G, Del Vecchio Blanco G, Cretella M, Paoluzi O, Corazza G, Pallone F, Monteleone G
    Mucosal Immunol, 2013;6(2):244-55.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Frozen
  6. Blood CD4+CD45RO+CXCR5+ T cells are decreased but partially functional in signal transducer and activator of transcription 3 deficiency.
    Authors: Mazerolles F, Picard C, Kracker S, Fischer A, Durandy A
    J Allergy Clin Immunol, 2013;131(4):1146-56, 1156.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells.
    Authors: Islam SA, Chang DS, Colvin RA
    Nat. Immunol., 2011;12(2):167-77.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Serum CXCL13 positively correlates with prostatic disease, prostate-specific antigen and mediates prostate cancer cell invasion, integrin clustering and cell adhesion.
    Authors: Singh S, Singh R, Sharma PK, Singh UP, Rai SN, Chung LW, Cooper CR, Novakovic KR, Grizzle WE, Lillard JW
    Cancer Lett., 2009;283(1):29-35.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  9. Chemokine CXCL13 is overexpressed in the tumour tissue and in the peripheral blood of breast cancer patients.
    Authors: Panse J, Friedrichs K, Marx A, Hildebrandt Y, Luetkens T, Bartels K, Horn C, Stahl T, Cao Y, Milde-Langosch K, Niendorf A, Kroger N, Wenzel S, Leuwer R, Bokemeyer C, Hegewisch-Becker S, Atanackovic D
    Br. J. Cancer, 2008;99(6):930-8.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  10. Altered expression of the receptor-ligand pair CXCR5/CXCL13 in B cells during chronic HIV-1 infection.
    Authors: Cagigi A, Mowafi F, Phuong Dang LV, Tenner-Racz K, Atlas A, Grutzmeier S, Racz P, Chiodi F, Nilsson A
    Blood, 2008;112(12):4401-10.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  11. The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
    Authors: Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J
    Stem Cells, 2007;25(7):1737-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. Overexpression of the CXCR5 chemokine receptor, and its ligand, CXCL13 in B-cell chronic lymphocytic leukemia.
    Authors: Burkle A, Niedermeier M, Schmitt-Graff A, Wierda WG, Keating MJ, Burger JA
    Blood, 2007;110(9):3316-25.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  13. Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells.
    Authors: Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH
    J. Immunol., 2007;179(6):3724-33.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  14. Hyaluronan-based polymer scaffold modulates the expression of inflammatory and degradative factors in mesenchymal stem cells: Involvement of Cd44 and Cd54.
    Authors: Lisignoli G, Cristino S, Piacentini A, Cavallo C, Caplan AI, Facchini A
    J. Cell. Physiol., 2006;207(2):364-73.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  15. Chemokine receptor expression in rat adjuvant-induced arthritis.
    Authors: Haas CS, Martinez RJ, Attia N, Haines GK, Campbell PL, Koch AE
    Arthritis Rheum., 2005;52(12):3718-30.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  16. Developing neonatal rabbit appendix, a primary lymphoid organ, is seeded by immature blood-borne B cells: evidence for roles for CD62L/PNAd, CCR7/CCL21, alpha4beta1 and LFA-1.
    Authors: Sinha RK, Mage RG
    Dev. Comp. Immunol., 2004;28(7):829-41.
    Species: Rabbit
    Sample Type: Whole Cells
    Application: Flow
  17. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
    Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
    J. Immunol., 2004;173(11):6592-602.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  18. Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses.
    Authors: Lim HW, Hillsamer P, Kim CH
    J. Clin. Invest., 2004;114(11):1640-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  19. Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
    Authors: Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T
    Blood, 2003;103(7):2806-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  20. Expression of functional CCR and CXCR chemokine receptors in podocytes.
    Authors: Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H
    J. Immunol., 2002;168(12):6244-52.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
Expand to show all 20 Citations
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