Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 51505
View all CXCR5 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CXCR5
Met1-Phe372
Accession # P32302

Specificity

Stains human CXCR5 transfectants but not the parental cell lines in flow cytometry. Does not cross-react with human CXCR2, CXCR3, or CXCR4 transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CXCR5 APC‑conjugated Antibody

Detection of CXCR5 antibody in CD19+Human PBMCs antibody by Flow Cytometry.

Detection of CXCR5 in CD19+Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Mouse Anti-Human CXCR5 APC-conjugated Monoclonal Antibody (Catalog # FAB190A) or (B) Mouse IgG2B control antibody (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Detection of CXCR5 antibody in CD4+Human PBMCs antibody by Flow Cytometry.

Detection of CXCR5 in CD4+Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse Anti-Human CXCR5 APC-conjugated Monoclonal Antibody (Catalog # FAB190A) or (B) Mouse IgG2B isotype control antibody (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CXCR5 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR5

CXCR5, also known as BLR-1, is a 7 transmembrane domain protein expressed on B cells. CXCR5 mediates B cell migration following binding of CXCL13.

Alternate Names

BLR1, CD185, CXCR5

Entrez Gene IDs

643 (Human); 12145 (Mouse); 29363 (Rat)

Gene Symbol

CXCR5

UniProt

P32302

Additional CXCR5 Products

Product Documents for Human CXCR5 APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CXCR5 APC‑conjugated Antibody

For research use only

Citations for Human CXCR5 APC‑conjugated Antibody

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