Human CXCR5 APC-conjugated Antibody

Clone 51505 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB190A-025
FAB190A-100
Human CXCR5 APC-conjugated Antibody in Flow Cytometry
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Product Details
Citations (6)
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Human CXCR5 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human CXCR5 transfectants but not the parental cell lines in flow cytometry. Does not cross-react with human CXCR2, CXCR3, or CXCR4 transfectants.
Source
Monoclonal Mouse IgG2B Clone # 51505
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CXCR5
Met1-Phe372
Accession # P32302
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection  of CXCR5 in CD19+ Human PBMCs by Flow Cytometry. View Larger

Detection of CXCR5 in CD19+ Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Mouse Anti-Human CXCR5 APC‑conjugated Monoclonal Antibody (Catalog # FAB190A) or (B) Mouse IgG2B control antibody (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins

Flow Cytometry Detection  of CXCR5 in CD4+ Human PBMCs by Flow Cytometry. View Larger

Detection of CXCR5 in CD4+ Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and either(A) Mouse Anti-Human CXCR5 APC-conjugated Monoclonal Antibody (Catalog # FAB190A) or (B) Mouse IgG2B isotype control antibody (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR5

CXCR5, also known as BLR-1, is a 7 transmembrane domain protein expressed on B cells. CXCR5 mediates B cell migration following binding of CXCL13.

Entrez Gene IDs
643 (Human); 12145 (Mouse); 29363 (Rat)
Alternate Names
BLR1; BLR1MDR-15; Burkitt lymphoma receptor 1; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif)receptor 5); Burkitt lymphoma receptor 1, GTP-binding protein; CD185 antigen; CD185; chemokine (C-X-C motif) receptor 5; C-X-C chemokine receptor type 5; CXCR5; CXC-R5; MDR15CXCR-5; MGC117347; Monocyte-derived receptor 15

Product Datasheets

Citations for Human CXCR5 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Role of CXCL13 and CCL20 in the recruitment of B cells to inflammatory foci in chronic arthritis
    Authors: E Armas-Gonz, MJ Domínguez-, A Díaz-Martí, M Arce-Franc, J Castro-Her, G Danelon, V Hernández-, S Bustabad-R, A Cantabrana, M Uguccioni, F Díaz-Gonzá
    Arthritis Res. Ther., 2018;20(1):114.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Evaluation of CD307a expression patterns during normal B-cell maturation and in B-cell malignancies by flow cytometry
    Authors: M Auat, CC Cardoso, IM Santos-Pir, RCM Rudolf-Oli, C Matiollo, BG Lange, JP da Silva, GC Dametto, MM Pirolli, MDHP Colombo, MC Santos-Sil
    Cytometry B Clin Cytom, 2018;0(0):.
    Species: Human
    Sample Types: Wbole Cells
    Applications: Flow Cytometry
  3. measles virus infection of human lymphocyte subsets demonstrates high susceptibility and permissiveness of both naive and memory B-cells
    Authors: BM Laksono, C Grosserich, RD de Vries, SAG Langeveld, MD Brem, JJM van Dongen, PD Katsikis, MPG Koopmans, MC van Zelm, RL de Swart
    J. Virol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Age-Dependent Pre-Vaccination Immunity Affects the Immunogenicity of Varicella Zoster Vaccination in Middle-aged Adults
    Authors: M van der He, LGH de Rond, MC van Zelm, GAM Berbers, AMH Boots, AM Buisman
    Front Immunol, 2018;9(0):46.
    Species: Human
    Sample Types: Whole Blood
    Applications: Flow Cytometry
  5. Differential effects of Cytomegalovirus carriage on the immune phenotype of middle-aged males and females
    Authors: Marieke van der He
    Sci Rep, 2016;6(0):26892.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells.
    Authors: Tso GH, Law HK, Tu W
    Stem Cells, 2010;28(5):939-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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