Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Flow Cytometry

Cited:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 56811
View all CXCR6 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CXCR6
Met1-Leu342
Accession # O00574

Specificity

Stains human CXCR6 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CXCR6 PE‑conjugated Antibody

Detection of CXCR6 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CXCR6 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) treated with 150 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) for 6 days were stained with Mouse Anti-Human CXCR6 PE-conjugated Monoclonal Antibody (Catalog # FAB699P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A). Quadrant markers were set based on control antibody staining (IC0041P). View our protocol for Staining Membrane-associated Proteins.

Detection of CXCR6 in HEK293 cells by Flow Cytometry.

HEK293 cells transfected with Human CXCR6 and eGFP (A) vs irrelevant and eGFP (B) were stained with Mouse Anti-Human CXCR6 PE‑conjugated Monoclonal Antibody (Catalog # FAB699P) at 37°C. View our protocol for Staining Membrane-associated Proteins.
Detection of Human CXCR6 by Flow Cytometry

Detection of Human CXCR6 by Flow Cytometry

CXCR6 and CXCL16 expression in LuCa cell lines(A) mRNA levels by semiquantitative RT-PCR. The copies of CXCR6 and CXCL16 transcripts are expressed relative to copies of 18S rRNA. Values are mean ± SEM from 3 independent experiments. *p ≤ 0.05, ***p ≤ 0.001 as compared to SCC (NCI-H520). (B) Flow cytometry analysis of CXCR6 and trans-membrane CXCL16 in (i) - SCC (NCI-H520) cells and (ii) - AC (NCI-H2126) cells. Grey dots represent isotype controls for PE and APC antibody and black dots represent CXCR6-PE and CXCL16-APC in SCC (NCI-H520) and AC (NCI-H2126). CXCR6 and CXCL16 both are expressed on ~82.9% (Q2) SCC cells; 5.41% (Q1) express only CXCR6 and only CXCL16 is expressed by 9.08% (Q3) of SCC cells. In AC cells, 61.7% of the population express both receptor and ligand (Q2) whereas, 11.1 (Q1) and 16.2% (Q3) cells express only CXCR6 and CXCL16, respectively. c) Levels of soluble CXCL16 in LuCa supernatant. Values are mean ± SEM from 3 independent experiments. ***p ≤ 0.0001 compared to NCI-H520. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.3194), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CXCR6 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with Recombinant Human IL‑2 (Catalog # 202-IL) and HEK293 cells transfected with Human CXCR6 and eGFP vs irrelevant

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR6

CXCR6, also known as Bonzo and STRL33, is a G protein coupled chemokine receptor expressed on activated T cells and tumor-infiltrating lymphocytes. It binds soluble CXCL16 and membrane-anchored CXCL16 expressed on dendritic cells and macrophages.

Alternate Names

Bonzo, CD186, CXCR6, STRL33

Entrez Gene IDs

10663 (Human); 80901 (Mouse); 100124593 (Rat)

Gene Symbol

CXCR6

UniProt

O00574

Additional CXCR6 Products

Product Documents for Human CXCR6 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CXCR6 PE‑conjugated Antibody

For research use only

Citations for Human CXCR6 PE‑conjugated Antibody

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