Human Cyclin D1 Antibody

Detection of Human, Mouse, and Rat Cyclin D1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, RAW 264.7 mouse monocyte/macrophage cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF Membrane was probed with 2 µg/mL of Human Cyclin D1 Monoclonal Antibody (Catalog # MAB43141) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cyclin D1 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Cyclin D1 in Human Breast Cancer Tissue. Cyclin D1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human Cyclin D1 Monoclonal Antibody (Catalog # MAB43141) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hemotoxylin (blue). Specific staining was localized to hair follicles and the cytoplasm and nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Cyclin D1 in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Human Cyclin D1 Monoclonal Antibody (Catalog # MAB43141, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with PFA and permeabilized with saponin.