Cytokeratin 19 antibodies are ideal for immunocytochemistry colocalization studies. The unconjugated antibody detects human Cytokeratin 19 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human Cytokeratin 14 is observed.
Polyclonal Sheep IgG
E. coli-derived recombinant human Cytokeratin 19 Thr2-Leu400 Accession # P08727
Supplied in a saline solution containing BSA and Sodium Azide.
Cytokeratin 19 in MCF‑7 Human Cell Line.
Cytokeratin 19 was detected in formaldehyde fixed MCF‑7 human breast cancer cell line using Sheep Anti-Human Cytokeratin 19 Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (green; Catalog # IC3506G) at 1:10 dilution overnight at 4 ° C and counterstained with DAPI (blue). Specific staining was localized to intermediate filaments. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Cytokeratin 19
Cytokeratin 19 (Keratin, type I cytoskeletal 19; also KRT-19, CK19 and Keratin-19) is a 40-45 kDa, acidic Class I keratin member of the intermediate filament family of proteins. Individual keratins are always expressed in tandem with a second keratin, and these are found in all epithelial cells. The class I KRT-19 heterodimerizes/polymerizes with 50-52 kDa class II KRT-8 (plus KRT-5 and -7) to form 8-10 nm filaments in epidermal stem cells, secretory gland (sweat; mammary; bile duct) simple epithelium, and neuroendocrine epidermal Merkel cells. It may represent a viable marker for skin stem cells. In skin, Cytokeratin 19 forms filaments in the fetal epithelium, and then progressively decreases with age, being virtually absent by age 17. Human Cytokeratin 19 is 400 amino acids (aa) in length. It contains an N-terminal "head" region (aa 1-79) and a subsequent "rod" region (aa 80-387), but is absent a typical C-terminal tail region. Cytokeratin 19 possesses at least 5 utilized phosphorylation sites plus one acetylated Lys residue. Based on other keratins, and the presence of an Asp at position 238, there may be caspase cleavage-generated isoforms. Full length human Cytokeratin 19 (aa 2-400) shares 82% aa sequence identity with mouse Cytokeratin 19.
Entrez Gene IDs:
3880 (Human); 16669 (Mouse)
CK19; CK-19; Cytokeratin 19; Cytokeratin-19,40-kDa keratin intermediate filament; EndoC; K19; K19cytokeratin-19; K1CS; keratin 19; keratin, type I cytoskeletal 19; keratin, type I, 40-kd; keratin-19; Krt19; MGC15366
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