Detection of Human Cytosolic beta ‑Glucosidase/GBA3 by Western Blot
Western blot shows lysates of human kidney (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Cytosolic beta ‑Glucosidase/GBA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5969) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cytosolic beta ‑Glucosidase/GBA3 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX.
Preparation and Storage
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytosolic beta-Glucosidase/GBA3
There are three beta-glucosidases (GBA) in human genome. GBA1 endodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glucosylceramide. GBA2 encodes a microsomal beta-glucosidase that catalyzes the hydrolysis of bile acid 3-O-glucosides. GBA3 is a cytosolic beta-glucosidase and is predominantly expressed in liver. GBA3 efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiological beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides. GBA3 also has significant neutral glycosylceramidase activity, suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism. At the protein level, GBA3 shows significant homology (>40%) with Klotho protein that is known for its association with aging process.
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