Dkk-1 (Dickkopf related protein 1) is a secreted antagonist of Wnt/beta-Catenin signaling. It interacts with LRP-6, and internalized complexes of Dkk-1/LRP-6/Kremen 2 can downregulate Wnt signaling. The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis. In addition, Dkk-1 can act as either a tumor suppressor or promoter of metastasis.
Human Dkk-1 Quantikine ELISA Kit
R&D Systems | Catalog # DKK100
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)
Sensitivity
15.6 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Human Dkk-1 Quantikine ELISA Kit
The Quantikine® Human Dkk-1 immunoassay is a 4.5 hour solid phase ELISA designed to measure human Dkk-1 in cell culture supernates, serum, and plasma. It contains
Sf
21-expressed recombinant human Dkk-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Dkk-1 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values of natural
human Dkk-1.
human Dkk-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human Dkk-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 223 | 600 | 1353 | 221 | 577 | 1287 |
| Standard Deviation | 9.4 | 15.3 | 36.5 | 16.9 | 35.0 | 69.6 |
| CV% | 4.2 | 2.6 | 2.7 | 7.7 | 6.1 | 5.4 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 212 | 599 | 1358 | 243 | 620 | 1371 |
| Standard Deviation | 8.9 | 24.5 | 44.4 | 18.4 | 37.4 | 63.1 |
| CV% | 4.2 | 4.1 | 3.3 | 7.3 | 6.0 | 4.6 |
Recovery for Human Dkk-1 Quantikine ELISA Kit
The recovery of human Dkk-1 spiked to various levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 97-109 |
| EDTA Plasma (n=4) | 94 | 86-102 |
| Heparin Plasma (n=4) | 98 | 92-106 |
| Serum (n=4) | 99 | 85-115 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Dkk-1 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Dkk-1 Quantikine ELISA Kit
Human Dkk-1 ELISA Standard Curve
Human Dkk-1 ELISA Cell Culture Supernate Standard Curve
Human Dkk-1 ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Dkk-1
Long Name
Dickkopf-1
Alternate Names
Dkk1
Gene Symbol
DKK1
Additional Dkk-1 Products
Product Documents for Human Dkk-1 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Dkk-1 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Human Dkk-1 Quantikine ELISA Kit
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Protocols
View specific protocols for Human Dkk-1 Quantikine ELISA Kit (DKK100):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
100 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- ELISA Troubleshooting Guide
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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