Detection of Human DNER by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DNER Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3646) followed by streptavidin-HRP. A specific band was detected for DNER at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
DNER (delta and notch-like EGF-related receptor; also BET) is a 120-150 kDa type I transmembrane glycoprotein that is reminescent of notch ligands such as Delta and Jagged. It is found on neuron dendrites and cell bodies but not on axons. Human DNER is 703 amino acids (aa) in length, and contains a 606 aa extracellular domain (ECD) (aa 35-640) plus a 76 aa cytoplasmic region. The ECD contains nine consecutive EGF-like domains, followed by one follistatin-like domain and a tenth EGF-like domain. DNER binding to notch-1 on glia induces morphological differentiation. Human DNER ECD is 89% aa identical to mouse DNER ECD.
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