Human DOCK3 Antibody

Catalog # Availability Size / Price Qty
AF7134
AF7134-SP
DOCK3 in Human Brain.
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Human DOCK3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DOCK3 in direct ELISAs. In direct ELISAs, less than 3% cross-reactivity with recombinant human (rh) DOCK1, rhDOCK2, and rhDOCK5 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human DOCK3
Gly418-Thr656
Accession # Q8IZD9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry DOCK3 in Human Brain. View Larger

DOCK3 in Human Brain. DOCK3 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain (cortex) using Sheep Anti-Human DOCK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7134) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hemotoxylin (blue). Specific staining was localized to neurofibrillary tangles. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DOCK3

DOCK3 (Dedicator of cytokinesis protein 3; also MOCA and PBP) is a 230-240 kDa member of the DOCK family, DOCK180 superfamily of proteins. DOCK3 expression appears to be restricted to neurons where it binds to Rac1 in conjunction with WAVE1, enhancing both Rac1 activity and that of downstream JNK. This results in cytoskeleton rearrangement and the promotion of axonal outgrowth. DOCK3 also binds to beta -catenin, thereby inhibiting Wnt signaling and promoting cell adhesion. Human DOCK3 is 2030 amino acids (aa) in length. It contains one protein-interaction SH3 domain (aa 7-650), a DHR-1 region that localizes DOCK3 to cell membranes (aa 418-654), a DHR-2 domain that likely binds to nucleotide-free GTPases and shows GEF activity (aa 1122-1630), and one SH3-binding motif (aa 1970‑1976). There is one phosphorylation site at Ser2013 and, unlike DOCK180, DOCK3 is known to form intracellular aggregates. There are three potential isoform variants. Two result from the use of alternative start sites at Met346 and Met388, while a third shows a deletion of aa 106‑123 coupled to a Gly substitution for aa 184‑2030. Over aa 418-656, human DOCK3 shares 99% aa identity with mouse DOCK3.

Long Name
Dedicator of Cytokinesis Protein 3
Entrez Gene IDs
1795 (Human); 208869 (Mouse); 315992 (Rat)
Alternate Names
dedicator of cytokinesis 3; DOCK3; KIAA0299; MOCA; modifier of cell adhesion

Product Datasheets

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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