Human Dopamine beta -Hydroxylase Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Dopamine beta ‑Hydroxylase in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Dopamine beta ‑Hydroxylase
    Leu37-Gly617
    Accession # P09172
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Dopamine beta ‑Hydroxylase by Western Blot. Western blot shows lysates of human adrenal gland tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Dopamine beta ‑Hydroxylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7376) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Dopamine beta ‑Hydroxylase at approximately 74-77 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dopamine beta-Hydroxylase
DBH (Dopamine beta -Hydroxylase) is a 77-78 kDa member of the Cu++ type II ascorbate-dependent monooxygenase family of enzymes. It is expressed in noradrenergic nerve terminals and adrenal medullary chromaffin cells, and serves as a catalyst for the conversion of dopamine into norepinepherine. Unlike its family member counterparts (TH and PNMT) that are cytosolic, DBH is embedded in the membranes of secretory vesicles. Human DBH is a 617 amino acid (aa) type II transmembrane glycoprotein (SwissProt # P09172). It contains an N-terminal 16 aa cytoplasmic region, plus a 580 aa lumenal domain (aa 38-617). The luminal region possesses one DOMON (dopamine beta -monooxygenase N-terminal) domain (aa 51-169) plus two consecutive monooxygenase motifs (aa 214-523). DBH exists as both a membrane-embedded 77-78 kDa isoform, and a soluble 73-75 kDa isoform. The latter may arise from cleavage following Gly39. Structurally, DBH will form a disulfide-linked homodimer, which then noncovalently associates with another DBH covalent homodimer, generating a functional 290 kDa homotetramer. Over aa 37‑617, human DBH shares 79% aa sequence identity with mouse DBH.
  • Entrez Gene IDs:
    1621 (Human); 13166 (Mouse); 25699 (Rat)
  • Alternate Names:
    DBH; DBM; dopamine beta-hydroxylase (dopamine beta-monooxygenase); Dopamine betaHydroxylase; Dopamine beta-Hydroxylase; Dopamine beta-monooxygenase; DOPBHY; EC 1.14.17.1
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