Human E-Cadherin Quantikine ELISA Kit

R&D Systems | Catalog # DCADE0

Product is in the process of discontinuation and replaced by catalog # DCADE0B.
R&D Systems
Discontinued Product
DCADE0 has been discontinued. An alternative/replacement product is available: DCADE0B. View all E-Cadherin products.

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (50 µL), Heparin Plasma (50 µL), Saliva (50 µL), Urine (50 µL)

Sensitivity

0.09 ng/mL

Assay Range

0.31-20 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine)
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Product Summary for Human E-Cadherin Quantikine ELISA Kit

The Quantikine Human E-Cadherin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human E-Cadherin in cell culture supernates, serum, plasma, urine, and saliva. It contains NS0-expressed recombinant human E-Cadherin/Fc Chimera and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human E-Cadherin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring E-Cadherin.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human E-Cadherin

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Heparin Plasma, Saliva, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 2.18 6.49 13.1 2.31 6.67 13.3
Standard Deviation 0.18 0.29 0.72 0.24 0.57 0.88
CV% 8.3 4.4 5.5 10.4 8.5 6.6

Recovery for Human E-Cadherin Quantikine ELISA Kit

The recovery of E-Cadherin spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 106 98-116

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of E-Cadherin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human E-Cadherin ELISA Linearity

Scientific Data Images for Human E-Cadherin Quantikine ELISA Kit

Human E-Cadherin ELISA Standard Curve

Human E-Cadherin ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: E-Cadherin

E-Cadherin/Cadherin-1 (ECAD) Is a transmembrane cell adhesion protein. It forms homophilic clusters between epithelial cells and is a critical component of adherens junctions. These junctions are critical for the maintenance of the epithelial barrier and epithelial tissue homeostasis. Decreased E-Cadherin is associated with physiological and pathological epithelial-to-mesenchymal transition (EMT) and cell migration, and E-Cadherin loss contributes to cancer metastasis. The shed ECD is released as a soluble factor that enhances cancer cell motility and EGFR-dependent survival and proliferation.

Alternate Names

Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin

Entrez Gene IDs

999 (Human); 12550 (Mouse); 83502 (Rat)

Gene Symbol

CDH1

Additional E-Cadherin Products

Product Documents for Human E-Cadherin Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human E-Cadherin Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human E-Cadherin Quantikine ELISA Kit

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  • Human E-Cadherin Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: in vitro cell culture
    Verified Customer | Posted 10/19/2019
  • Human E-Cadherin Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: in vitro cell lysis
    Verified Customer | Posted 08/13/2019
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    Name: Anonymous
    Sample Tested: Pancreatic cancer cells
    Verified Customer | Posted 10/19/2018

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Protocols

View specific protocols for Human E-Cadherin Quantikine ELISA Kit (DCADE0):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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