Human E-Cadherin Quantikine ELISA Kit

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Human E-Cadherin ELISA Standard Curve
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Product Details
Procedure
Citations (9)
FAQs
Reviews (3)

Human E-Cadherin Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), Heparin Plasma (50 uL), Saliva (50 uL), Urine (50 uL)
Sensitivity
0.09 ng/mL
Assay Range
0.3 - 20 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine)
Specificity
Natural and recombinant human E-Cadherin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human E-Cadherin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human E-Cadherin in cell culture supernates, serum, plasma, urine, and saliva. It contains NS0-expressed recombinant human E-Cadherin/Fc Chimera and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human E-Cadherin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring E-Cadherin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 2.18 6.49 13.1 2.31 6.67 13.3
Standard Deviation 0.18 0.29 0.72 0.24 0.57 0.88
CV% 8.3 4.4 5.5 10.4 8.5 6.6

Recovery

The recovery of E-Cadherin spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 106 98-116

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of E-Cadherin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human E-Cadherin ELISA Linearity

Data Examples

Human E-Cadherin ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: E-Cadherin

The cadherin superfamily comprises a large number of cell surface glycoproteins with one or more cadherin repeats, which are involved in Ca2+-dependent cell-cell adhesion. Classical cadherins contain five extracellular cadherin repeats and a conserved cytoplasmic region that interacts with the actin cytoskeleton via catenins. In addition to cell adhesion, cadherins also mediate morphogenesis, cytoskeletal organization, and cell migration. In addition to the classical cadherins, the cadherin superfamily also includes desmosomal cadherins, protocadherins, 7TM cadherins, FAT family cadherins, and T-cadherin.

Entrez Gene IDs:
999 (Human); 12550 (Mouse); 83502 (Rat)
Alternate Names:
Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human E-Cadherin Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Doublecortin-like kinase 1 promotes hepatocyte clonogenicity and oncogenic programming via non-canonical &beta-catenin-dependent mechanism
    Authors: N Ali, CB Nguyen, P Chandrakes, RF Wolf, D Qu, R May, T Goretsky, J Fazili, TA Barrett, M Li, MM Huycke, MS Bronze, CW Houchen
    Sci Rep, 2020;10(1):10578.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Targeting the temporal dynamics of hypoxia-induced tumor-secreted factors halts tumor migration
    Authors: M Singh, XJ Tian, VS Donnenberg, AM Watson, J Zhang, LP Stabile, SC Watkins, J Xing, S Sant
    Cancer Res., 2019;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Structure establishment of three-dimensional (3D) cell culture printing model for bladder cancer
    Authors: MJ Kim, BH Chi, JJ Yoo, YM Ju, YM Whang, IH Chang
    PLoS ONE, 2019;14(10):e0223689.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Meloxicam executes its antitumor effects against hepatocellular carcinoma in COX-2- dependent and -independent pathways.
    Authors: Dong, Xiaofeng, Li, Rui, Xiu, Peng, Dong, Xuesong, Xu, Zongzhen, Zhai, Bo, Liu, Feng, Jiang, Hongchi, Sun, Xueying, Li, Jie, Qiao, Haiquan
    PLoS ONE, 2014;9(3):e92864.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Polymorphonuclear neutrophils promote dyshesion of tumor cells and elastase-mediated degradation of E-cadherin in pancreatic tumors.
    Authors: Gaida M, Steffen T, Gunther F, Tschaharganeh D, Felix K, Bergmann F, Schirmacher P, Hansch G
    Eur J Immunol, 2012;42(12):3369-80.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Serum levels of beta-catenin as a potential marker for genotype 4/hepatitis C-associated hepatocellular carcinoma.
    Authors: Zekri A, Bahnassy A, Alam El-Din H, Morsy H, Shaarawy S, Moharram N, Daoud S
    Oncol Rep, 2011;26(4):825-31.
    Species: Human
    Sample Types: Serum
  7. Epithelial adhesion molecules can inhibit HIV-1-specific CD8+ T-cell functions.
    Authors: Streeck H, Kwon DS, Pyo A, Flanders M, Chevalier MF, Law K, Julg B, Trocha K, Jolin JS, Anahtar MN, Lian J, Toth I, Brumme Z, Chang JJ, Caron T, Rodig SJ, Milner DA, Piechoka-Trocha A, Kaufmann DE, Walker BD, Altfeld M
    Blood, 2011;117(19):5112-22.
    Species: Human
    Sample Types: Plasma
  8. Plasma protein growth arrest-specific 6 levels are associated with altered glucose tolerance, inflammation, and endothelial dysfunction.
    Authors: Hung YJ, Lee CH, Chu NF
    Diabetes Care, 2010;33(8):1840-4.
    Species: Human
    Sample Types: Plasma
  9. Elevated serum levels of soluble E-cadherin in patients with primary Sjogren's syndrome.
    Authors: Jonsson MV, Salomonsson S, Oijordsbakken G, Skarstein K
    Scand. J. Immunol., 2005;62(6):552-9.
    Species: Human
    Sample Types: Serum

FAQs

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Reviews for Human E-Cadherin Quantikine ELISA Kit

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Human E-Cadherin Quantikine ELISA Kit
By Anonymous on 10/19/2019
Sample Tested: in vitro cell culture

Human E-Cadherin Quantikine ELISA Kit
By Anonymous on 08/13/2019
Sample Tested: in vitro cell lysis

Human E-Cadherin Quantikine ELISA Kit
By Anonymous on 10/19/2018
Sample Tested: Pancreatic cancer cells