Human E-Selectin/CD62E Biotinylated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    This antibody stains COS cells transfected with cDNA for human E-Selectin/CD62E in immunofluorescence experiments. This antibody does not stain COS cells transfected with cDNA for human ICAM-1, PECAM-1 or VCAM-1.
  • Source
    Monoclonal Mouse IgG1 Clone # BBIG-E5
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Activated HUVEC human umbilical vein endothelial cells
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human E-Selectin/CD62E Fc Chimera (Catalog # 724-ES)
    under non-reducing conditions only
  • Immunocytochemistry
    8-25 µg/mL
    See below
    • Human E-Selectin/CD62E Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human E‑Selectin/CD62E Antibody (Catalog # BBA2)
  • ELISA Capture (Matched Antibody Pair)
    2-10 µg/mL 
    Human E‑Selectin/CD62E Antibody (Catalog # BBA16)
  • ELISA Detection (Matched Antibody Pair)
    0.5-2.0 µg/mL 
    Human E‑Selectin/CD62E Biotinylated Antibody (Catalog # BBA8)
  • ELISA Standard
     
    Recombinant Human E-Selectin/CD62E Protein, CF (Catalog # ADP1)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
E-Selectin/CD62E in HUVEC Cells. E-Selectin/CD62E was detected in immersion fixed human umbilical vein endothelial cells (HUVECs) activated with TNF alpha using Mouse Anti-Human E‑Selectin/CD62E Biotinylated Monoclonal Antibody (Catalog # BBA8) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: E-Selectin/CD62E

E-Selectin, also known as Endothelial Leukocyte Adhesion Molecule 1 (ELAM-1), and Leukocyte-Endothelial Cell Adhesion Molecule 2 (LECAM-2), has been designated CD62E. It is a cell-surface glycoprotein expressed by activated endothelium and it mediates the adhesion of blood neutrophils.

  • Entrez Gene IDs:
    6401 (Human); 20339 (Mouse); 25544 (Rat)
  • Alternate Names:
    CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E
Related Research Areas
Isotype Controls
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