Human E-Selectin/CD62E Biotinylated Antibody

    
Datasheet / CoA / SDS
Product Details
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Human
  • Specificity
    This antibody stains COS cells transfected with cDNA for human E-Selectin/CD62E in immunofluorescence experiments. This antibody does not stain COS cells transfected with cDNA for human ICAM-1, PECAM-1 or VCAM-1.
  • Source
    Monoclonal Mouse IgG1 Clone # BBIG-E5
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Activated HUVEC human umbilical vein endothelial cells
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human E-Selectin/CD62E Fc Chimera (Catalog # 724-ES)
    under non-reducing conditions only
  • Immunocytochemistry
    8-25 µg/mL
    See below
    • Human E-Selectin/CD62E Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human E‑Selectin/CD62E Antibody (Catalog # BBA2 )
  • ELISA Detection (Matched Antibody Pair)
    0.5-2.0 µg/mL 
    Human E‑Selectin/CD62E Biotinylated Antibody (Catalog # BBA8 )
  • ELISA Standard
     
    Recombinant Human E-Selectin/CD62E Protein, CF (Catalog # ADP1 )
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Immunocytochemistry
E-Selectin/CD62E in HUVEC Cells. E-Selectin/CD62E was detected in immersion fixed human umbilical vein endothelial cells (HUVECs) activated with TNF alpha using Mouse Anti-Human E‑Selectin/CD62E Biotinylated Monoclonal Antibody (Catalog # BBA8) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: E-Selectin/CD62E

E-Selectin, also known as Endothelial Leukocyte Adhesion Molecule 1 (ELAM-1), and Leukocyte-Endothelial Cell Adhesion Molecule 2 (LECAM-2), has been designated CD62E. It is a cell-surface glycoprotein expressed by activated endothelium and it mediates the adhesion of blood neutrophils.

  • Entrez Gene IDs:
    6401 (Human); 20339 (Mouse); 25544 (Rat)
  • Alternate Names:
    CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E
Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Biotinylated Antibody

Ctrl IC002B 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Streptavidin-Phycoerythrin

Flow F0040 7
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Streptavidin NL557

Flow , IHC , ICC NL999 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Streptavidin-Allophycocyanin

Flow F0050 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Streptavidin NL637

Flow , IHC , ICC NL998 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Streptavidin NL493

Flow , IHC , ICC NL997 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Avidin-Fluorescein

Flow F0030 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Supplemental Cell Selection Products
Description Application Cat# Citations Images  

MagCellect Streptavidin Ferrofluid

MAG999B 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

MagCellect Streptavidin Ferrofluid

MAG999 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP

ELISA DY998 51  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

EvenCoat Streptavidin Coated Plates

ELISA CP004
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human E-Selectin/CD62E Biotinylated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Us to Order
reload

Feedback

Customer Information
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies
R&D Systems Guarantee