Human E-Selectin/CD62E Antibody

Clone BBIG-E4 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
BBA16
Human E-Selectin/CD62E Antibody in Immunocytochemistry
2 Images
Product Details
Citations (39)
FAQs
Supplemental Products
Reviews (1)

Human E-Selectin/CD62E Antibody Summary

Species Reactivity
Human
Specificity
Detects E‑Selectin/CD62E in ELISAs and Western blots. In immunofluorescence experiments, does not stain COS cells transfected with cDNA for human ICAM-1, PECAM‑1, L-Selectin, P-Selectin, or VCAM-1.
Source
Monoclonal Mouse IgG1 Clone # BBIG-E4
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Activated HUVEC human umbilical vein endothelial cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human E-Selectin/CD62E Fc Chimera (Catalog # 724-ES) under non-reducing conditions only
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
Pigott, R. et al. (1991) J. Immunol. 147:130.
 
Immunocytochemistry
8-25 µg/mL
See below

Human E-Selectin/CD62E Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-10 µg/mL 

Use in combination with:

Detection Reagent: Human E‑Selectin/CD62E Biotinylated Antibody (Catalog # BBA8)

Standard: Recombinant Human E-Selectin/CD62E Protein, CF (Catalog # ADP1)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry E-Selectin/CD62E in HUVEC Human Cells. View Larger

E-Selectin/CD62E in HUVEC Human Cells. E-Selectin/CD62E was detected in immersion fixed HUVEC human umbilical vein endothelial cells activated with TNF-a (Catalog # 210-TA-010) using Mouse Anti-Human E-Selectin/ CD62E Monoclonal Antibody (Catalog # BBA16) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry E‑Selectin/CD62E in Human Brain. View Larger

E‑Selectin/CD62E in Human Brain. E-Selectin/CD62E was detected in immersion fixed paraffin-embedded sections of human brain (occipital cortex) using 10 µg/mL Mouse Anti-Human E-Selectin/CD62E Monoclonal Antibody (Catalog # BBA16) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: E-Selectin/CD62E

E-Selectin, also known as Endothelial Leukocyte Adhesion Molecule 1 (ELAM-1), and Leukocyte-Endothelial Cell Adhesion Molecule 2 (LECAM-2), has been designated CD62E. It is a cell-surface glycoprotein expressed by activated endothelium and it mediates the adhesion of blood neutrophils.

Entrez Gene IDs
6401 (Human); 20339 (Mouse); 25544 (Rat)
Alternate Names
CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E

Product Datasheets

Citations for Human E-Selectin/CD62E Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. A Symphytum officinale Root Extract Exerts Anti-inflammatory Properties by Affecting Two Distinct Steps of NF-?B Signaling
    Authors: J Seigner, M Junker-Sam, A Plaza, G D'Urso, M Masullo, S Piacente, YM Holper-Sch, R de Martin
    Front Pharmacol, 2019;10(0):289.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA - Cell Based
  2. A modular platform for targeted RNAi therapeutics
    Authors: R Kedmi, N Veiga, S Ramishetti, M Goldsmith, D Rosenblum, N Dammes, I Hazan-Hale, L Nahary, S Leviatan-B, M Harlev, M Behlke, I Benhar, J Lieberman, D Peer
    Nat Nanotechnol, 2018;0(0):.
  3. Optimizing human Treg immunotherapy by Treg subset selection and E-selectin ligand expression
    Authors: C Donnelly, B Dykstra, N Mondal, J Huang, BJ Kaskow, R Griffin, R Sackstein, C Baecher-Al
    Sci Rep, 2018;8(1):420.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Pinocembrin protects endothelial cells from oxidized LDL-induced injury
    Authors: Q Su, Y Sun, Z Ye, H Yang, B Kong, L Li
    Cytokine, 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Analysis of neuronal phosphoproteome reveals PINK1 regulation of BAD function and cell death
    Authors: H Wan, B Tang, X Liao, Q Zeng, Z Zhang, L Liao
    Cell Death Differ., 2017;0(0):.
  6. Localized delivery of curcumin from injectable gelatin/Thai silk fibroin microspheres for anti-inflammatory treatment of osteoarthritis in a rat model
    Authors: J Ratanavara, K Soontornvi, S Shuangshot, S Shuangshot, S Damrongsak
    Inflammopharmacology, 2017;0(0):.
  7. The Abl-1 kinase is dispensable for NK cell inhibitory signaling and is not involved in murine NK cell education
    Authors: S Ganesan, TL Thanh, N Kadri, BJ Chambers, S Meinke, P Brodin, E Vivier, DM Wetzel, AJ Koleske, P Höglund
    Scand. J. Immunol., 2017;0(0):.
  8. Aliskiren has chondroprotective efficacy in a rat model of osteoarthritis through suppression of the local renin-angiotensin system
    Authors: K Yan, Y Shen
    Mol Med Rep, 2017;0(0):.
  9. Heme oxygenase-1 directly binds STAT3 to control the generation of pathogenic Th17 cells during neutrophilic airway inflammation
    Authors: X Lin, J Lv, J Lv, C Di, Y Zhang, T Zhou, J Liu, Z Xia
    Allergy, 2017;0(0):.
  10. A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer
    Authors: BV Howley, LA Link, S Grelet, M El-Sabban, PH Howe
    Oncogene, 2017;0(0):.
  11. Bordetella pertussis adenylate cyclase toxin disrupts functional integrity of bronchial epithelial layers
    Authors: S Hasan, NN Kulkarni, A Asbjarnars, I Linhartova, R Osicka, P Sebo, GH Gudmundsso
    Infect. Immun., 2017;0(0):.
  12. Hepatic inflammation-fibrosis-cancer axis in the rat hepatocellular carcinoma induced by diethylnitrosamine
    Authors: YF Ding, ZH Wu, YJ Wei, L Shu, YR Peng
    J. Cancer Res. Clin. Oncol, 2017;0(0):.
  13. Erythropoietin signaling increases neurogenesis and oligodendrogenesis of endogenous neural stem cells following spinal cord injury both in�vivo and in�vitro
    Authors: H Zhang, X Fang, D Huang, Q Luo, M Zheng, K Wang, L Cao, Z Yin
    Mol Med Rep, 2017;0(0):.
  14. Purinergic receptor transactivation by the ?2-adrenergic receptor increases intracellular Ca(2+) in non-excitable cells
    Authors: W Stallaert, ET van der We, AM Schonegge, B Plouffe, M Hogue, V Lukashova, A Inoue, S Ishida, J Aoki, C Le Gouill, M Bouvier
    Mol. Pharmacol, 2017;0(0):.
  15. AGER gene polymorphisms and soluble receptor for advanced glycation end product in patients with idiopathic pulmonary fibrosis
    Authors: K Yamaguchi, H Iwamoto, Y Horimasu, S Ohshimo, K Fujitaka, H Hamada, W Mazur, N Kohno, N Hattori
    Respirology, 2017;0(0):.
  16. Amine oxidase activity regulates the development of pulmonary fibrosis
    Authors: F Marttila-I, K Elima, K Auvinen, TZ Veres, P Rantakari, C Weston, M Miyasaka, D Adams, S Jalkanen, M Salmi
    FASEB J, 2017;0(0):.
  17. Phenotypic and Functional Alterations of Hematopoietic Stem and Progenitor Cells in an In Vitro Leukemia-Induced Microenvironment
    Authors: JP Vernot, X Bonilla, V Rodriguez-, NP Vanegas
    Int J Mol Sci, 2017;18(2):.
  18. Blockade of interleukin-6 receptor in the periphery promotes rapid and sustained antidepressant actions: a possible role of gut-microbiota-brain axis
    Authors: JC Zhang, W Yao, C Dong, C Yang, Q Ren, M Ma, K Hashimoto
    Transl Psychiatry, 2017;7(5):e1138.
  19. Anti-CD20 monoclonal antibody combined with adenovirus vector-mediated IL-10 regulates spleen CD4+/CD8+ T�cells and T-bet/GATA-3 expression in NOD mice
    Authors: A Tang, C Li, Z Chen, T Li
    Mol Med Rep, 2017;0(0):.
  20. Rat astrocytes are more supportive for mouse OPC self-renewal than mouse astrocytes in culture
    Authors: Xuejun Cheng
    Dev Neurobiol, 2016;0(0):.
  21. 3D co-culture model to analyze the crosstalk between endothelial and smooth muscle cells
    Authors: Minu Karthika Ganesan
    Tissue Eng Part C Methods, 2016;0(0):.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: ICC
  22. Protection of Dexmedetomidine Against Ischemia/Reperfusion-Induced Apoptotic Insults to Neuronal Cells Occurs via an Intrinsic Mitochondria-Dependent Pathway
    Authors: Gong-Jhe Wu
    J. Cell. Biochem, 2016;0(0):.
  23. Plasma membrane TLR activation increases bacterial uptake but abrogates endosomal Lactobacillus acidophilus induction of Interferon-B
    Immunology, 2016;0(0):.
  24. Endothelin-1 Overexpression Exaggerates Diabetes-Induced Endothelial Dysfunction by Altering Oxidative Stress
    Am J Hypertens, 2016;0(0):.
  25. Soluble ST2 Predicts 1-Year Outcome in Patients Undergoing Transcatheter Aortic Valve Implantation (TAVI)
    Authors: Bernhard Wernly
    Eur. J. Clin. Invest, 2016;0(0):.
  26. Presence of vascular endothelial growth factor during the first half of IVM improves the meiotic and developmental competence of porcine oocytes from small follicles
    Authors: Tra M T Bui
    Reprod. Fertil. Dev, 2016;0(0):.
  27. Cell surface-anchored syndecan-1 ameliorates intestinal inflammation and neutrophil transmigration in ulcerative colitis
    J Cell Mol Med, 2016;0(0):.
  28. IL-17RB(+) granulocytes are associated with airflow obstruction in asthma
    Authors: Lin Li
    Ann. Allergy Asthma Immunol, 2016;117(6):674-679.
  29. Role of Epigenetic Modification and Immunomodulation in a Murine Prostate Cancer Model
    Authors: Georgi Guruli
    Prostate, 2016;0(0):.
  30. Leukemia Mediated Endothelial Cell Activation Modulates Leukemia Cell Susceptibility to Chemotherapy through a Positive Feedback Loop Mechanism.
    Authors: Pezeshkian, Bahareh, Donnelly, Christop, Tamburo, Kelley, Geddes, Timothy, Madlambayan, Gerard J
    PLoS ONE, 2013;8(4):e60823.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  31. Endothelial cells of human colorectal cancer and healthy colon reveal phenotypic differences in culture.
    Authors: Schellerer VS, Croner RS, Weinlander K, Hohenberger W, Sturzl M, Naschberger E
    Lab. Invest., 2007;87(11):1159-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  32. An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
    Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
    J. Exp. Med., 2006;203(12):2763-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  33. Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB.
    Authors: Amin MA, Haas CS, Zhu K, Mansfield PJ, Kim MJ, Lackowski NP, Koch AE
    Blood, 2006;107(6):2252-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  34. Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
    Authors: Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK
    Blood, 2006;108(3):928-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development
  35. A role for the L-selectin adhesion system in mediating cytotrophoblast emigration from the placenta.
    Authors: Prakobphol A, Genbacev O, Gormley M, Kapidzic M, Fisher SJ
    Dev. Biol., 2006;298(1):107-17.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  36. The role of endothelial cell reactive antibodies in peripheral arterial disease.
    Authors: Armitage JD, Lindsey NJ, Homer-Vanniasinkam S
    Eur J Vasc Endovasc Surg, 2005;31(2):170-5.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  37. Differential effects of orbital and laminar shear stress on endothelial cells.
    Authors: Frattini J, Kudo FA
    J. Vasc. Surg., 2005;41(5):869-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  38. Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts.
    Authors: Helantera I, Loginov R, Koskinen P, Tornroth T, Gronhagen-Riska C, Lautenschlager I
    Nephrol. Dial. Transplant., 2005;20(4):790-6.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  39. Endothelium properties of a tissue-engineered blood vessel for small-diameter vascular reconstruction.
    Authors: Remy-Zolghadri M, Laganiere J, Oligny JF, Germain L, Auger FA
    J. Vasc. Surg., 2004;39(3):613-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human E-Selectin/CD62E Antibody

Average Rating: 3 (Based on 1 Review)

5 Star
0%
4 Star
0%
3 Star
100%
2 Star
0%
1 Star
0%

Have you used Human E-Selectin/CD62E Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human E-Selectin/CD62E Antibody
By Anonymous on 04/26/2017
Application: ICC/IF Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human

HUVECs were stimulated with LPS for activation