Human E-Selectin/CD62E Antibody

Name: Human E-Selectin/CD62E Antibody
Brand: R&D Systems
Rating: 3 (1 reviews)

Clone BBIG-E4 was used by HLDA to establish CD designation

(35 citations)

(1 Review)

Datasheet / CoA / SDS

E-Selectin/CD62E in HUVEC Human Cells.<BR>

Immunocytochemistry

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Immunohistochemistry

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Product Details

Citations (35)

FAQs

Supplemental Products

Reviews

Species Reactivity

Human
Specificity

Detects E-Selectin/CD62E in ELISAs and Western blots. In immunofluorescence experiments, does not stain COS cells transfected with cDNA for human ICAM-1, PECAM-1, L-Selectin, P-Selectin, or VCAM-1.
Source

Monoclonal Mouse IgG₁ Clone # BBIG-E4
Purification

Protein A or G purified from hybridoma culture supernatant
Immunogen

Activated HUVEC human umbilical vein endothelial cells
Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.
Label

Unconjugated

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human E-Selectin/CD62E Fc Chimera (Catalog # 724-ES) under non-reducing conditions only
Immunohistochemistry

8-25 µg/mL

See below
Immunoprecipitation

Pigott, R. et al. (1991) J. Immunol. 147:130.
Immunocytochemistry

8-25 µg/mL

See below

Human E-Selectin/CD62E Sandwich Immunoassay

Reagent

ELISA Capture (Matched Antibody Pair)

2-10 µg/mL

Human E‑Selectin/CD62E Antibody (Catalog # BBA16 )

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Human E‑Selectin/CD62E Biotinylated Antibody (Catalog # BBA8 )

ELISA Standard

Recombinant Human E-Selectin/CD62E Protein, CF (Catalog # ADP1 )

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry

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E-Selectin/CD62E in HUVEC Human Cells.

E-Selectin/CD62E was detected in immersion fixed HUVEC human umbilical vein endothelial cells activated with TNF‑ alpha (Catalog # 210-TA-010) using Mouse Anti-Human E‑Selectin/
CD62E Monoclonal Antibody (Catalog # BBA16) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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E‑Selectin/CD62E in Human Brain.

E‑Selectin/CD62E was detected in immersion fixed paraffin-embedded sections of human brain (occipital cortex) using 10 µg/mL Mouse Anti-Human E‑Selectin/CD62E Monoclonal Antibody (Catalog # BBA16) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Background: E-Selectin/CD62E

E-Selectin, also known as Endothelial Leukocyte Adhesion Molecule 1 (ELAM-1), and Leukocyte-Endothelial Cell Adhesion Molecule 2 (LECAM-2), has been designated CD62E. It is a cell-surface glycoprotein expressed by activated endothelium and it mediates the adhesion of blood neutrophils.

Entrez Gene IDs:

6401 (Human); 20339 (Mouse); 25544 (Rat)

Alternate Names:

CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
Filter your results:

Species	Applications	Sample Type
Optimizing human Treg immunotherapy by Treg subset selection and E-selectin ligand expression Authors: C Donnelly, B Dykstra, N Mondal, J Huang, BJ Kaskow, R Griffin, R Sackstein, C Baecher-Al Sci Rep, 2018;8(1):420. Species: Human Sample Type: Cell Lysates Application: WB A modular platform for targeted RNAi therapeutics Authors: R Kedmi, N Veiga, S Ramishetti, M Goldsmith, D Rosenblum, N Dammes, I Hazan-Hale, L Nahary, S Leviatan-B, M Harlev, M Behlke, I Benhar, J Lieberman, D Peer Nat Nanotechnol, 2018;0(0):. Heme oxygenase-1 directly binds STAT3 to control the generation of pathogenic Th17 cells during neutrophilic airway inflammation Authors: X Lin, J Lv, J Lv, C Di, Y Zhang, T Zhou, J Liu, Z Xia Allergy, 2017;0(0):. Anti-CD20 monoclonal antibody combined with adenovirus vector-mediated IL-10 regulates spleen CD4+/CD8+ Tï¿½cells and T-bet/GATA-3 expression in NOD mice Authors: A Tang, C Li, Z Chen, T Li Mol Med Rep, 2017;0(0):. The Abl-1 kinase is dispensable for NK cell inhibitory signaling and is not involved in murine NK cell education Authors: S Ganesan, TL Thanh, N Kadri, BJ Chambers, S Meinke, P Brodin, E Vivier, DM Wetzel, AJ Koleske, P Höglund Scand. J. Immunol., 2017;0(0):. Purinergic receptor transactivation by the ?2-adrenergic receptor increases intracellular Ca(2+) in non-excitable cells Authors: W Stallaert, ET van der We, AM Schonegge, B Plouffe, M Hogue, V Lukashova, A Inoue, S Ishida, J Aoki, C Le Gouill, M Bouvier Mol. Pharmacol, 2017;0(0):. Localized delivery of curcumin from injectable gelatin/Thai silk fibroin microspheres for anti-inflammatory treatment of osteoarthritis in a rat model Authors: J Ratanavara, K Soontornvi, S Shuangshot, S Shuangshot, S Damrongsak Inflammopharmacology, 2017;0(0):. Hepatic inflammation-fibrosis-cancer axis in the rat hepatocellular carcinoma induced by diethylnitrosamine Authors: YF Ding, ZH Wu, YJ Wei, L Shu, YR Peng J. Cancer Res. Clin. Oncol, 2017;0(0):. AGER gene polymorphisms and soluble receptor for advanced glycation end product in patients with idiopathic pulmonary fibrosis Authors: K Yamaguchi, H Iwamoto, Y Horimasu, S Ohshimo, K Fujitaka, H Hamada, W Mazur, N Kohno, N Hattori Respirology, 2017;0(0):. A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer Authors: BV Howley, LA Link, S Grelet, M El-Sabban, PH Howe Oncogene, 2017;0(0):. Analysis of neuronal phosphoproteome reveals PINK1 regulation of BAD function and cell death Authors: H Wan, B Tang, X Liao, Q Zeng, Z Zhang, L Liao Cell Death Differ., 2017;0(0):. Erythropoietin signaling increases neurogenesis and oligodendrogenesis of endogenous neural stem cells following spinal cord injury both inï¿½vivo and inï¿½vitro Authors: H Zhang, X Fang, D Huang, Q Luo, M Zheng, K Wang, L Cao, Z Yin Mol Med Rep, 2017;0(0):. Aliskiren has chondroprotective efficacy in a rat model of osteoarthritis through suppression of the local renin-angiotensin system Authors: K Yan, Y Shen Mol Med Rep, 2017;0(0):. Bordetella pertussis adenylate cyclase toxin disrupts functional integrity of bronchial epithelial layers Authors: S Hasan, NN Kulkarni, A Asbjarnars, I Linhartova, R Osicka, P Sebo, GH Gudmundsso Infect. Immun., 2017;0(0):. Phenotypic and Functional Alterations of Hematopoietic Stem and Progenitor Cells in an In Vitro Leukemia-Induced Microenvironment Authors: JP Vernot, X Bonilla, V Rodriguez-, NP Vanegas Int J Mol Sci, 2017;18(2):. 3D co-culture model to analyze the crosstalk between endothelial and smooth muscle cells Authors: Minu Karthika Ganesan Tissue Eng Part C Methods, 2016;0(0):. Species: Human Sample Type: Complex Sample Type Application: ICC Plasma membrane TLR activation increases bacterial uptake but abrogates endosomal Lactobacillus acidophilus induction of Interferon-B Immunology, 2016;0(0):. Role of Epigenetic Modification and Immunomodulation in a Murine Prostate Cancer Model Authors: Georgi Guruli Prostate, 2016;0(0):. Endothelin-1 Overexpression Exaggerates Diabetes-Induced Endothelial Dysfunction by Altering Oxidative Stress Am J Hypertens, 2016;0(0):. Cell surface-anchored syndecan-1 ameliorates intestinal inflammation and neutrophil transmigration in ulcerative colitis J Cell Mol Med, 2016;0(0):. IL-17RB(+) granulocytes are associated with airflow obstruction in asthma Authors: Lin Li Ann. Allergy Asthma Immunol, 2016;117(6):674-679. Protection of Dexmedetomidine Against Ischemia/Reperfusion-Induced Apoptotic Insults to Neuronal Cells Occurs via an Intrinsic Mitochondria-Dependent Pathway Authors: Gong-Jhe Wu J. Cell. Biochem, 2016;0(0):. Rat astrocytes are more supportive for mouse OPC self-renewal than mouse astrocytes in culture Authors: Xuejun Cheng Dev Neurobiol, 2016;0(0):. Soluble ST2 Predicts 1-Year Outcome in Patients Undergoing Transcatheter Aortic Valve Implantation (TAVI) Authors: Bernhard Wernly Eur. J. Clin. Invest, 2016;0(0):. Presence of vascular endothelial growth factor during the first half of IVM improves the meiotic and developmental competence of porcine oocytes from small follicles Authors: Tra M T Bui Reprod. Fertil. Dev, 2016;0(0):. Leukemia Mediated Endothelial Cell Activation Modulates Leukemia Cell Susceptibility to Chemotherapy through a Positive Feedback Loop Mechanism. Authors: Pezeshkian, Bahareh, Donnelly, Christop, Tamburo, Kelley, Geddes, Timothy, Madlambayan, Gerard J PLoS ONE, 2013;8(4):e60823. Species: Human Sample Type: Whole Cells Application: Functional Assay Endothelial cells of human colorectal cancer and healthy colon reveal phenotypic differences in culture. Authors: Schellerer VS, Croner RS, Weinlander K, Hohenberger W, Sturzl M, Naschberger E Lab. Invest., 2007;87(11):1159-70. Species: Human Sample Type: Whole Cells Application: ICC Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes. Authors: Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK Blood, 2006;108(3):928-35. Species: Human Sample Type: Whole Cells Application: ELISA Development A role for the L-selectin adhesion system in mediating cytotrophoblast emigration from the placenta. Authors: Prakobphol A, Genbacev O, Gormley M, Kapidzic M, Fisher SJ Dev. Biol., 2006;298(1):107-17. Species: Human Sample Type: Whole Tissue Application: IHC Frozen An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium. Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG J. Exp. Med., 2006;203(12):2763-77. Species: Human Sample Type: Whole Cells Application: ICC Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB. Authors: Amin MA, Haas CS, Zhu K, Mansfield PJ, Kim MJ, Lackowski NP, Koch AE Blood, 2006;107(6):2252-61. Species: Human Sample Type: Whole Cells Application: Neut Differential effects of orbital and laminar shear stress on endothelial cells. Authors: Frattini J, Kudo FA J. Vasc. Surg., 2005;41(5):869-80. Species: Human Sample Type: Whole Cells Application: ICC Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts. Authors: Helantera I, Loginov R, Koskinen P, Tornroth T, Gronhagen-Riska C, Lautenschlager I Nephrol. Dial. Transplant., 2005;20(4):790-6. Species: Human Sample Type: Whole Tissue Application: IHC The role of endothelial cell reactive antibodies in peripheral arterial disease. Authors: Armitage JD, Lindsey NJ, Homer-Vanniasinkam S Eur J Vasc Endovasc Surg, 2005;31(2):170-5. Species: Human Sample Type: Serum Application: ELISA Development Endothelium properties of a tissue-engineered blood vessel for small-diameter vascular reconstruction. Authors: Remy-Zolghadri M, Laganiere J, Oligny JF, Germain L, Auger FA J. Vasc. Surg., 2004;39(3):613-20. Species: Human Sample Type: Whole Cells Application: Flow Expand to show all Citations

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Cell and Tissue Staining Kits

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Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

CTS002 15

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Human E-Selectin/CD62E Antibody

Certificate of Analysis Lookup

Certificate of Analysis Lookup

Certificate of Analysis Request Form

FAQs

Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

ExactaChIP Chromatin IP Buffer Panel

Antigen Retrieval Reagent-Universal

Antigen Retrieval Reagent-Basic

Mounting Medium

Antigen Retrieval Reagent Sampler (50 mL each)

Antigen Retrieval Reagent-Acidic

VisUCyte Antigen Retrieval Reagent-Basic

NorthernLights Guard Mounting Media

VisUCyte Antigen Retrieval Reagent-Universal

VisUCyte Antigen Retrieval Reagent-Acidic

Mouse IgG 1 Isotype Control

Mouse IgG1 Isotype Control

Reconstitution Buffer 1 (PBS)

Mouse IgG HRP-conjugated Antibody

Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Mouse IgG HRP-conjugated Antibody

Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Mouse IgG Antibody

Donkey Anti-Mouse IgG Biotinylated Antibody

Goat Anti-Mouse IgG Biotinylated Antibody

Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

Mouse IgG VisUCyte HRP Polymer Antibody

Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Donkey Anti-Mouse IgG (H+L) Affinity Purified PAb, X Absorb

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Mouse IgG₁ Isotype Control