Human E-Selectin/CD62E Antibody

Clone BBIG-E4 was used by HLDA to establish CD designation
  (35 citations)
(1 Review)
    
Datasheet / CoA / SDS
Product Details
Citations (35)
FAQs
Supplemental Products
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  • Species Reactivity
    Human
  • Specificity
    Detects E-Selectin/CD62E in ELISAs and Western blots. In immunofluorescence experiments, does not stain COS cells transfected with cDNA for human ICAM-1, PECAM-1, L-Selectin, P-Selectin, or VCAM-1.
  • Source
    Monoclonal Mouse IgG1 Clone # BBIG-E4
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Activated HUVEC human umbilical vein endothelial cells
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human E-Selectin/CD62E Fc Chimera (Catalog # 724-ES) under non-reducing conditions only
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Immunoprecipitation
    Pigott, R. et al. (1991) J. Immunol. 147:130.
  • Immunocytochemistry
    8-25 µg/mL
    See below
    • Human E-Selectin/CD62E Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-10 µg/mL 
    Human E‑Selectin/CD62E Antibody (Catalog # BBA16 )
  • ELISA Detection (Matched Antibody Pair)
    0.5-2.0 µg/mL 
    Human E‑Selectin/CD62E Biotinylated Antibody (Catalog # BBA8 )
  • ELISA Standard
     
    Recombinant Human E-Selectin/CD62E Protein, CF (Catalog # ADP1 )
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
E-Selectin/CD62E in HUVEC Human Cells.

E-Selectin/CD62E was detected in immersion fixed HUVEC human umbilical vein endothelial cells activated with TNF‑ alpha (Catalog # 210-TA-010) using Mouse Anti-Human E‑Selectin/
CD62E Monoclonal Antibody (Catalog # BBA16) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry
E‑Selectin/CD62E in Human Brain.

E‑Selectin/CD62E was detected in immersion fixed paraffin-embedded sections of human brain (occipital cortex) using 10 µg/mL Mouse Anti-Human E‑Selectin/CD62E Monoclonal Antibody (Catalog # BBA16) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
      12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: E-Selectin/CD62E
E-Selectin, also known as Endothelial Leukocyte Adhesion Molecule 1 (ELAM-1), and Leukocyte-Endothelial Cell Adhesion Molecule 2 (LECAM-2), has been designated CD62E. It is a cell-surface glycoprotein expressed by activated endothelium and it mediates the adhesion of blood neutrophils.
  • Entrez Gene IDs:
    6401 (Human); 20339 (Mouse); 25544 (Rat)
  • Alternate Names:
    CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Optimizing human Treg immunotherapy by Treg subset selection and E-selectin ligand expression
    Authors: C Donnelly, B Dykstra, N Mondal, J Huang, BJ Kaskow, R Griffin, R Sackstein, C Baecher-Al
    Sci Rep, 2018;8(1):420.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. A modular platform for targeted RNAi therapeutics
    Authors: R Kedmi, N Veiga, S Ramishetti, M Goldsmith, D Rosenblum, N Dammes, I Hazan-Hale, L Nahary, S Leviatan-B, M Harlev, M Behlke, I Benhar, J Lieberman, D Peer
    Nat Nanotechnol, 2018;0(0):.
  3. Heme oxygenase-1 directly binds STAT3 to control the generation of pathogenic Th17 cells during neutrophilic airway inflammation
    Authors: X Lin, J Lv, J Lv, C Di, Y Zhang, T Zhou, J Liu, Z Xia
    Allergy, 2017;0(0):.
  4. Anti-CD20 monoclonal antibody combined with adenovirus vector-mediated IL-10 regulates spleen CD4+/CD8+ T�cells and T-bet/GATA-3 expression in NOD mice
    Authors: A Tang, C Li, Z Chen, T Li
    Mol Med Rep, 2017;0(0):.
  5. The Abl-1 kinase is dispensable for NK cell inhibitory signaling and is not involved in murine NK cell education
    Authors: S Ganesan, TL Thanh, N Kadri, BJ Chambers, S Meinke, P Brodin, E Vivier, DM Wetzel, AJ Koleske, P Höglund
    Scand. J. Immunol., 2017;0(0):.
  6. Purinergic receptor transactivation by the ?2-adrenergic receptor increases intracellular Ca(2+) in non-excitable cells
    Authors: W Stallaert, ET van der We, AM Schonegge, B Plouffe, M Hogue, V Lukashova, A Inoue, S Ishida, J Aoki, C Le Gouill, M Bouvier
    Mol. Pharmacol, 2017;0(0):.
  7. Localized delivery of curcumin from injectable gelatin/Thai silk fibroin microspheres for anti-inflammatory treatment of osteoarthritis in a rat model
    Authors: J Ratanavara, K Soontornvi, S Shuangshot, S Shuangshot, S Damrongsak
    Inflammopharmacology, 2017;0(0):.
  8. Hepatic inflammation-fibrosis-cancer axis in the rat hepatocellular carcinoma induced by diethylnitrosamine
    Authors: YF Ding, ZH Wu, YJ Wei, L Shu, YR Peng
    J. Cancer Res. Clin. Oncol, 2017;0(0):.
  9. AGER gene polymorphisms and soluble receptor for advanced glycation end product in patients with idiopathic pulmonary fibrosis
    Authors: K Yamaguchi, H Iwamoto, Y Horimasu, S Ohshimo, K Fujitaka, H Hamada, W Mazur, N Kohno, N Hattori
    Respirology, 2017;0(0):.
  10. A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer
    Authors: BV Howley, LA Link, S Grelet, M El-Sabban, PH Howe
    Oncogene, 2017;0(0):.
  11. Analysis of neuronal phosphoproteome reveals PINK1 regulation of BAD function and cell death
    Authors: H Wan, B Tang, X Liao, Q Zeng, Z Zhang, L Liao
    Cell Death Differ., 2017;0(0):.
  12. Erythropoietin signaling increases neurogenesis and oligodendrogenesis of endogenous neural stem cells following spinal cord injury both in�vivo and in�vitro
    Authors: H Zhang, X Fang, D Huang, Q Luo, M Zheng, K Wang, L Cao, Z Yin
    Mol Med Rep, 2017;0(0):.
  13. Aliskiren has chondroprotective efficacy in a rat model of osteoarthritis through suppression of the local renin-angiotensin system
    Authors: K Yan, Y Shen
    Mol Med Rep, 2017;0(0):.
  14. Bordetella pertussis adenylate cyclase toxin disrupts functional integrity of bronchial epithelial layers
    Authors: S Hasan, NN Kulkarni, A Asbjarnars, I Linhartova, R Osicka, P Sebo, GH Gudmundsso
    Infect. Immun., 2017;0(0):.
  15. Phenotypic and Functional Alterations of Hematopoietic Stem and Progenitor Cells in an In Vitro Leukemia-Induced Microenvironment
    Authors: JP Vernot, X Bonilla, V Rodriguez-, NP Vanegas
    Int J Mol Sci, 2017;18(2):.
  16. 3D co-culture model to analyze the crosstalk between endothelial and smooth muscle cells
    Authors: Minu Karthika Ganesan
    Tissue Eng Part C Methods, 2016;0(0):.
    Species: Human
    Sample Type: Complex Sample Type
    Application: ICC
  17. Plasma membrane TLR activation increases bacterial uptake but abrogates endosomal Lactobacillus acidophilus induction of Interferon-B
    Immunology, 2016;0(0):.
  18. Role of Epigenetic Modification and Immunomodulation in a Murine Prostate Cancer Model
    Authors: Georgi Guruli
    Prostate, 2016;0(0):.
  19. Endothelin-1 Overexpression Exaggerates Diabetes-Induced Endothelial Dysfunction by Altering Oxidative Stress
    Am J Hypertens, 2016;0(0):.
  20. Cell surface-anchored syndecan-1 ameliorates intestinal inflammation and neutrophil transmigration in ulcerative colitis
    J Cell Mol Med, 2016;0(0):.
  21. IL-17RB(+) granulocytes are associated with airflow obstruction in asthma
    Authors: Lin Li
    Ann. Allergy Asthma Immunol, 2016;117(6):674-679.
  22. Protection of Dexmedetomidine Against Ischemia/Reperfusion-Induced Apoptotic Insults to Neuronal Cells Occurs via an Intrinsic Mitochondria-Dependent Pathway
    Authors: Gong-Jhe Wu
    J. Cell. Biochem, 2016;0(0):.
  23. Rat astrocytes are more supportive for mouse OPC self-renewal than mouse astrocytes in culture
    Authors: Xuejun Cheng
    Dev Neurobiol, 2016;0(0):.
  24. Soluble ST2 Predicts 1-Year Outcome in Patients Undergoing Transcatheter Aortic Valve Implantation (TAVI)
    Authors: Bernhard Wernly
    Eur. J. Clin. Invest, 2016;0(0):.
  25. Presence of vascular endothelial growth factor during the first half of IVM improves the meiotic and developmental competence of porcine oocytes from small follicles
    Authors: Tra M T Bui
    Reprod. Fertil. Dev, 2016;0(0):.
  26. Leukemia Mediated Endothelial Cell Activation Modulates Leukemia Cell Susceptibility to Chemotherapy through a Positive Feedback Loop Mechanism.
    Authors: Pezeshkian, Bahareh, Donnelly, Christop, Tamburo, Kelley, Geddes, Timothy, Madlambayan, Gerard J
    PLoS ONE, 2013;8(4):e60823.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  27. Endothelial cells of human colorectal cancer and healthy colon reveal phenotypic differences in culture.
    Authors: Schellerer VS, Croner RS, Weinlander K, Hohenberger W, Sturzl M, Naschberger E
    Lab. Invest., 2007;87(11):1159-70.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  28. Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
    Authors: Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK
    Blood, 2006;108(3):928-35.
    Species: Human
    Sample Type: Whole Cells
    Application: ELISA Development
  29. A role for the L-selectin adhesion system in mediating cytotrophoblast emigration from the placenta.
    Authors: Prakobphol A, Genbacev O, Gormley M, Kapidzic M, Fisher SJ
    Dev. Biol., 2006;298(1):107-17.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  30. An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
    Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
    J. Exp. Med., 2006;203(12):2763-77.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  31. Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB.
    Authors: Amin MA, Haas CS, Zhu K, Mansfield PJ, Kim MJ, Lackowski NP, Koch AE
    Blood, 2006;107(6):2252-61.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  32. Differential effects of orbital and laminar shear stress on endothelial cells.
    Authors: Frattini J, Kudo FA
    J. Vasc. Surg., 2005;41(5):869-80.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  33. Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts.
    Authors: Helantera I, Loginov R, Koskinen P, Tornroth T, Gronhagen-Riska C, Lautenschlager I
    Nephrol. Dial. Transplant., 2005;20(4):790-6.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  34. The role of endothelial cell reactive antibodies in peripheral arterial disease.
    Authors: Armitage JD, Lindsey NJ, Homer-Vanniasinkam S
    Eur J Vasc Endovasc Surg, 2005;31(2):170-5.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
  35. Endothelium properties of a tissue-engineered blood vessel for small-diameter vascular reconstruction.
    Authors: Remy-Zolghadri M, Laganiere J, Oligny JF, Germain L, Auger FA
    J. Vasc. Surg., 2004;39(3):613-20.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Expand to show all Citations

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Average Rating: 3 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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 ICC/IF Human Anonymous 04/26/2017
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Summary

Application Immunocytochemistry/Immunofluorescence
Sample Tested HUVEC human umbilical vein endothelial cells
Species Human

Other Experimental Details

Other Experimental Details HUVECs were stimulated with LPS for activation

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