Human E-Selectin/CD62E Antibody

Name: Human E-Selectin/CD62E Antibody
Brand: R&D Systems
SKU: BBA16
Rating: 4.3 (3 reviews)

BBA16 39 Citations 3 Reviews Datasheet / COA / SDS

Clone BBIG-E4 was used by HLDA to establish CD designation

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BBA16

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Detection of E-Selectin/CD62E in HUVECs by Flow Cytometry.

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Citations (39)

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Reviews (3)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human E-Selectin/CD62E Antibody Summary

Species Reactivity

Human

Specificity

Detects E‑Selectin/CD62E in ELISAs and Western blots. In immunofluorescence experiments, does not stain COS cells transfected with cDNA for human ICAM-1, PECAM‑1, L-Selectin, P-Selectin, or VCAM-1.

Source

Monoclonal Mouse IgG₁ Clone # BBIG-E4

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Activated HUVEC human umbilical vein endothelial cells

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human E-Selectin/CD62E Fc Chimera (Catalog # 724-ES) under non-reducing conditions only

Flow Cytometry

0.25 µg/10⁶ cells

Human umbilical cord endothelial cells (HUVECs) cultured for 6 hours in the presence of 25 ng/mL of rhTNF-alpha (Catalog # 210-TA)

Immunohistochemistry

8-25 µg/mL

See below

Immunoprecipitation

Pigott, R. et al. (1991) J. Immunol. 147:130.

Immunocytochemistry

8-25 µg/mL

See below

Human E-Selectin/CD62E Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-10 µg/mL

Use in combination with:

Detection Reagent: Human E‑Selectin/CD62E Biotinylated Antibody (Catalog # BBA8)

Standard: Recombinant Human E-Selectin/CD62E Protein, CF (Catalog # ADP1)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry

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Detection of E-Selectin/CD62E in HUVECs by Flow Cytometry. Human umbilical cord endothelial cells (HUVECs) were cultured for 6 hours in the presence of 25 ng/mL of rhTNF-alpha (210-TA, filled histogram) or rested (open histogram) and stained with Mouse anti-human E-selectin/CD62E (BBA16) followed by Goat anti-Mouse APC-conjugated secondary antibody (F0101B). Gates were set based on isotype control (MAB002, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

Immunocytochemistry

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E-Selectin/CD62E in HUVEC Human Cells. E-Selectin/CD62E was detected in immersion fixed HUVEC human umbilical vein endothelial cells activated with TNF-a (Catalog # 210-TA-010) using Mouse Anti-Human E-Selectin/ CD62E Monoclonal Antibody (Catalog # BBA16) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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E‑Selectin/CD62E in Human Brain. E-Selectin/CD62E was detected in immersion fixed paraffin-embedded sections of human brain (occipital cortex) using 10 µg/mL Mouse Anti-Human E-Selectin/CD62E Monoclonal Antibody (Catalog # BBA16) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: E-Selectin/CD62E

E-Selectin, also known as Endothelial Leukocyte Adhesion Molecule 1 (ELAM-1), and Leukocyte-Endothelial Cell Adhesion Molecule 2 (LECAM-2), has been designated CD62E. It is a cell-surface glycoprotein expressed by activated endothelium and it mediates the adhesion of blood neutrophils.

Entrez Gene IDs

6401 (Human); 20339 (Mouse); 25544 (Rat)

Alternate Names

CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human E-Selectin/CD62E Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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A Symphytum officinale Root Extract Exerts Anti-inflammatory Properties by Affecting Two Distinct Steps of NF-?B Signaling
Authors: J Seigner, M Junker-Sam, A Plaza, G D'Urso, M Masullo, S Piacente, YM Holper-Sch, R de Martin
Front Pharmacol, 2019;10(0):289.
Species: Human
Sample Types: Whole Cells
Applications: ELISA - Cell Based
A modular platform for targeted RNAi therapeutics
Authors: R Kedmi, N Veiga, S Ramishetti, M Goldsmith, D Rosenblum, N Dammes, I Hazan-Hale, L Nahary, S Leviatan-B, M Harlev, M Behlke, I Benhar, J Lieberman, D Peer
Nat Nanotechnol, 2018;0(0):.
Optimizing human Treg immunotherapy by Treg subset selection and E-selectin ligand expression
Authors: C Donnelly, B Dykstra, N Mondal, J Huang, BJ Kaskow, R Griffin, R Sackstein, C Baecher-Al
Sci Rep, 2018;8(1):420.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Pinocembrin protects endothelial cells from oxidized LDL-induced injury
Authors: Q Su, Y Sun, Z Ye, H Yang, B Kong, L Li
Cytokine, 2018;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Analysis of neuronal phosphoproteome reveals PINK1 regulation of BAD function and cell death
Authors: H Wan, B Tang, X Liao, Q Zeng, Z Zhang, L Liao
Cell Death Differ., 2017;0(0):.
Localized delivery of curcumin from injectable gelatin/Thai silk fibroin microspheres for anti-inflammatory treatment of osteoarthritis in a rat model
Authors: J Ratanavara, K Soontornvi, S Shuangshot, S Shuangshot, S Damrongsak
Inflammopharmacology, 2017;0(0):.
The Abl-1 kinase is dispensable for NK cell inhibitory signaling and is not involved in murine NK cell education
Authors: S Ganesan, TL Thanh, N Kadri, BJ Chambers, S Meinke, P Brodin, E Vivier, DM Wetzel, AJ Koleske, P Höglund
Scand. J. Immunol., 2017;0(0):.
Aliskiren has chondroprotective efficacy in a rat model of osteoarthritis through suppression of the local renin-angiotensin system
Authors: K Yan, Y Shen
Mol Med Rep, 2017;0(0):.
Heme oxygenase-1 directly binds STAT3 to control the generation of pathogenic Th17 cells during neutrophilic airway inflammation
Authors: X Lin, J Lv, J Lv, C Di, Y Zhang, T Zhou, J Liu, Z Xia
Allergy, 2017;0(0):.
A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer
Authors: BV Howley, LA Link, S Grelet, M El-Sabban, PH Howe
Oncogene, 2017;0(0):.

Bordetella pertussis adenylate cyclase toxin disrupts functional integrity of bronchial epithelial layers
Authors: S Hasan, NN Kulkarni, A Asbjarnars, I Linhartova, R Osicka, P Sebo, GH Gudmundsso
Infect. Immun., 2017;0(0):.

Hepatic inflammation-fibrosis-cancer axis in the rat hepatocellular carcinoma induced by diethylnitrosamine
Authors: YF Ding, ZH Wu, YJ Wei, L Shu, YR Peng
J. Cancer Res. Clin. Oncol, 2017;0(0):.

Erythropoietin signaling increases neurogenesis and oligodendrogenesis of endogenous neural stem cells following spinal cord injury both inï¿½vivo and inï¿½vitro
Authors: H Zhang, X Fang, D Huang, Q Luo, M Zheng, K Wang, L Cao, Z Yin
Mol Med Rep, 2017;0(0):.

Purinergic receptor transactivation by the ?2-adrenergic receptor increases intracellular Ca(2+) in non-excitable cells
Authors: W Stallaert, ET van der We, AM Schonegge, B Plouffe, M Hogue, V Lukashova, A Inoue, S Ishida, J Aoki, C Le Gouill, M Bouvier
Mol. Pharmacol, 2017;0(0):.

AGER gene polymorphisms and soluble receptor for advanced glycation end product in patients with idiopathic pulmonary fibrosis
Authors: K Yamaguchi, H Iwamoto, Y Horimasu, S Ohshimo, K Fujitaka, H Hamada, W Mazur, N Kohno, N Hattori
Respirology, 2017;0(0):.

Amine oxidase activity regulates the development of pulmonary fibrosis
Authors: F Marttila-I, K Elima, K Auvinen, TZ Veres, P Rantakari, C Weston, M Miyasaka, D Adams, S Jalkanen, M Salmi
FASEB J, 2017;0(0):.

Phenotypic and Functional Alterations of Hematopoietic Stem and Progenitor Cells in an In Vitro Leukemia-Induced Microenvironment
Authors: JP Vernot, X Bonilla, V Rodriguez-, NP Vanegas
Int J Mol Sci, 2017;18(2):.

Blockade of interleukin-6 receptor in the periphery promotes rapid and sustained antidepressant actions: a possible role of gut-microbiota-brain axis
Authors: JC Zhang, W Yao, C Dong, C Yang, Q Ren, M Ma, K Hashimoto
Transl Psychiatry, 2017;7(5):e1138.

Anti-CD20 monoclonal antibody combined with adenovirus vector-mediated IL-10 regulates spleen CD4+/CD8+ Tï¿½cells and T-bet/GATA-3 expression in NOD mice
Authors: A Tang, C Li, Z Chen, T Li
Mol Med Rep, 2017;0(0):.

Rat astrocytes are more supportive for mouse OPC self-renewal than mouse astrocytes in culture
Authors: Xuejun Cheng
Dev Neurobiol, 2016;0(0):.

3D co-culture model to analyze the crosstalk between endothelial and smooth muscle cells
Authors: Minu Karthika Ganesan
Tissue Eng Part C Methods, 2016;0(0):.
Species: Human
Sample Types: Complex Sample Type
Applications: ICC

Protection of Dexmedetomidine Against Ischemia/Reperfusion-Induced Apoptotic Insults to Neuronal Cells Occurs via an Intrinsic Mitochondria-Dependent Pathway
Authors: Gong-Jhe Wu
J. Cell. Biochem, 2016;0(0):.

Plasma membrane TLR activation increases bacterial uptake but abrogates endosomal Lactobacillus acidophilus induction of Interferon-B
Immunology, 2016;0(0):.

Endothelin-1 Overexpression Exaggerates Diabetes-Induced Endothelial Dysfunction by Altering Oxidative Stress
Am J Hypertens, 2016;0(0):.

Soluble ST2 Predicts 1-Year Outcome in Patients Undergoing Transcatheter Aortic Valve Implantation (TAVI)
Authors: Bernhard Wernly
Eur. J. Clin. Invest, 2016;0(0):.

Presence of vascular endothelial growth factor during the first half of IVM improves the meiotic and developmental competence of porcine oocytes from small follicles
Authors: Tra M T Bui
Reprod. Fertil. Dev, 2016;0(0):.

Cell surface-anchored syndecan-1 ameliorates intestinal inflammation and neutrophil transmigration in ulcerative colitis
J Cell Mol Med, 2016;0(0):.

IL-17RB(+) granulocytes are associated with airflow obstruction in asthma
Authors: Lin Li
Ann. Allergy Asthma Immunol, 2016;117(6):674-679.

Role of Epigenetic Modification and Immunomodulation in a Murine Prostate Cancer Model
Authors: Georgi Guruli
Prostate, 2016;0(0):.

Leukemia Mediated Endothelial Cell Activation Modulates Leukemia Cell Susceptibility to Chemotherapy through a Positive Feedback Loop Mechanism.
Authors: Pezeshkian, Bahareh, Donnelly, Christop, Tamburo, Kelley, Geddes, Timothy, Madlambayan, Gerard J
PLoS ONE, 2013;8(4):e60823.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Endothelial cells of human colorectal cancer and healthy colon reveal phenotypic differences in culture.
Authors: Schellerer VS, Croner RS, Weinlander K, Hohenberger W, Sturzl M, Naschberger E
Lab. Invest., 2007;87(11):1159-70.
Species: Human
Sample Types: Whole Cells
Applications: ICC

An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
J. Exp. Med., 2006;203(12):2763-77.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB.
Authors: Amin MA, Haas CS, Zhu K, Mansfield PJ, Kim MJ, Lackowski NP, Koch AE
Blood, 2006;107(6):2252-61.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
Authors: Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK
Blood, 2006;108(3):928-35.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Development

A role for the L-selectin adhesion system in mediating cytotrophoblast emigration from the placenta.
Authors: Prakobphol A, Genbacev O, Gormley M, Kapidzic M, Fisher SJ
Dev. Biol., 2006;298(1):107-17.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

The role of endothelial cell reactive antibodies in peripheral arterial disease.
Authors: Armitage JD, Lindsey NJ, Homer-Vanniasinkam S
Eur J Vasc Endovasc Surg, 2005;31(2):170-5.
Species: Human
Sample Types: Serum
Applications: ELISA Development

Differential effects of orbital and laminar shear stress on endothelial cells.
Authors: Frattini J, Kudo FA
J. Vasc. Surg., 2005;41(5):869-80.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts.
Authors: Helantera I, Loginov R, Koskinen P, Tornroth T, Gronhagen-Riska C, Lautenschlager I
Nephrol. Dial. Transplant., 2005;20(4):790-6.
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Endothelium properties of a tissue-engineered blood vessel for small-diameter vascular reconstruction.
Authors: Remy-Zolghadri M, Laganiere J, Oligny JF, Germain L, Auger FA
J. Vasc. Surg., 2004;39(3):613-20.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry