Human EGF Quantikine ELISA Kit

  (45 citations)
(1 Review)
    
Datasheet / CoA / SDS
Product Details
Assay Procedure
Citations (45)
FAQs
Supplemental Products
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    3.5 hours or 4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (200 uL), Serum (10 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Platelet-poor Citrate Plasma (200 uL), Urine (10 uL)
  • Sensitivity
    0.7 pg/mL
  • Assay Range
    3.9 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma, Urine)
  • Specificity
    Natural and recombinant human EGF
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC21 , Quantikine Immunoassay Control Group 4 - Please Inquire
Product Summary
The Quantikine Human EGF Immunoassay kit is a 3.5 or 4.5 hour solid phase ELISA designed to measure EGF levels in cell culture supernates, serum, plasma, and urine. It contains E. coli-derived recombinant human EGF and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human EGF. Results obtained measuring natural human EGF showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural EGF.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Urine
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 15 78.9 134 15 80 136
Standard Deviation 0.4 2.5 4 0.9 4.5 6.8
CV% 2.7 3.2 3 6 5.6 5

Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 13.5 80 133 14.7 77 134
Standard Deviation 0.6 2 2.7 0.9 3.3 6.1
CV% 4.4 2.5 2 6.1 4.3 4.6

Recovery

The recovery of EGF spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 98 88-111
Citrate Plasma (n=10) 104 89-115
EDTA Plasma (n=10) 100 80-111
Heparin Plasma (n=10) 96 79-108
Serum (n=10) 97 80-106
Urine (n=1) 102 97-108
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of EGF in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 EGF [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: EGF
EGF (Epidermal Growth Factor) is expressed as a transmembrane protein that is proteolytically cleaved to generate soluble forms. It signals through EGF R/ErbB1 which heterodimerizes with ErbB2, ErbB3, or ErbB4 and can also associate with PDGF R beta and HGF R/c-MET. During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult, EGF plays a role in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration.
  • Long Name:
    Epidermal Growth Factor
  • Entrez Gene IDs:
    1950 (Human); 13645 (Mouse); 25313 (Rat)
  • Alternate Names:
    beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent for Serum & Plasma Samples Only
  4.   For Serum & Plasma Samples Only: Add 50 µL of Assay Diluent to each well.

  5. 200 µL Standard, Control, or Sample
  6.   Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well.
  10.   For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    For Cell Culture Supernate & Urine Samples: Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  11.   Aspirate and wash 3 times.

  12. 200 µL Substrate Solution
  13.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

  14. 50 µL Stop Solution
  15. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

45 Citations: Showing 1 - 10
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Species
Sample Type
  1. Therapeutic cancer vaccine: phase I clinical tolerance study of Hu-rhEGF-rP64k/Mont in patients with newly diagnosed advanced non-small cell lung cancer
    Authors: P Xing, H Wang, S Yang, X Han, Y Sun, Y Shi
    BMC Immunol., 2018;19(1):14.
    Species: Human
    Sample Type: Serum
  2. The p38 signaling pathway mediates quiescence of glioma stem cells by regulating epidermal growth factor receptor trafficking
    Authors: A Soeda, J Lathia, BJ Williams, Q Wu, J Gallagher, A Androutsel, AJ Giles, C Yang, Z Zhuang, MR Gilbert, JN Rich, DM Park
    Oncotarget, 2017;8(20):33316-33328.
    Species: Human
    Sample Type: Cell Culture Supernates
  3. The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro
    Authors: M Bernardi, F Agostini, K Chieregato, E Amati, C Durante, M Rassu, M Ruggeri, S Sella, E Lombardi, M Mazzucato, G Astori
    J Transl Med, 2017;15(1):90.
    Species: Human
    Sample Type: Cell Lysates
  4. Inflammatory Differences in Plaque Erosion and Rupture in Patients With ST-Segment Elevation Myocardial Infarction
    Authors: S Chandran, J Watkins, A Abdul-Aziz, M Shafat, PA Calvert, KM Bowles, MD Flather, SA Rushworth, AD Ryding
    J Am Heart Assoc, 2017;6(5):.
    Species: Human
    Sample Type: Plasma
  5. Umbilical cord extracts improve diabetic abnormalities in bone marrow-derived mesenchymal stem cells and increase their therapeutic effects on diabetic nephropathy
    Authors: K Nagaishi, Y Mizue, T Chikenji, M Otani, M Nakano, Y Saijo, H Tsuchida, S Ishioka, A Nishikawa, T Saito, M Fujimiya
    Sci Rep, 2017;7(1):8484.
    Species: Human
    Sample Type: Cell Culture Supernates
  6. Differential effects of amnion and chorion membrane extracts on osteoblast-like cells due to the different growth factor composition of the extracts
    Authors: YY Go, SE Kim, GJ Cho, SW Chae, JJ Song
    PLoS ONE, 2017;12(8):e0182716.
    Species: Human
    Sample Type: Tissue Homogenates
  7. Design, characterization and experimental validation of a compact, flexible pulsed power architecture for ex vivo platelet activation
    Authors: AL Garner, A Caiafa, Y Jiang, S Klopman, C Morton, AS Torres, AM Loveless, VB Neculaes
    PLoS ONE, 2017;12(7):e0181214.
    Species: Human
    Sample Type: Plasma
  8. N-Glycosylation of integrin ?5 acts as a switch for EGFR-mediated complex formation of integrin ?5?1 to ?6?4
    Sci Rep, 2016;6(0):33507.
    Species: Human
    Sample Type: Cell Culture Supernates
  9. Urine Epidermal Growth Factor, Monocyte Chemoattractant Protein-1 or Their Ratio as Biomarkers for Interstitial Fibrosis and Tubular Atrophy in Primary Glomerulonephritis
    Authors: Supanat Worawichaw
    Kidney Blood Press. Res, 2016;41(6):997-1007.
    Species: Human
    Sample Type: Urine
  10. Serum Interleukin-18, Fetuin-A, Soluble Intercellular Adhesion Molecule-1, and Endothelin-1 in Ankylosing Spondylitis, Psoriatic Arthritis, and SAPHO Syndrome
    Int J Mol Sci, 2016;17(8):.
    Species: Human
    Sample Type: Serum
  11. Transgenic Soybean Production of Bioactive Human Epidermal Growth Factor (EGF)
    Authors: Yonghua He
    PLoS ONE, 2016;11(6):e0157034.
    Species: Plant - Arabidopsis
    Sample Type: Protein
  12. Adipose-derived mesenchymal stem cells from patients with atherosclerotic renovascular disease have increased DNA damage and reduced angiogenesis that can be modified by hypoxia
    Stem Cell Res Ther, 2016;7(1):128.
    Species: Human
    Sample Type: Cell Culture Supernates
  13. Endothelial cell-secreted EGF induces epithelial to mesenchymal transition and endows head and neck cancer cells with stem-like phenotype.
    Authors: Zhang Z, Dong Z, Lauxen I, Filho M, Nor J
    Cancer Res, 2014;74(10):2869-81.
    Species: Human
    Sample Type: Cell Culture Supernates
  14. Ex vivo expansion of bovine corneal endothelial cells in xeno-free medium supplemented with platelet releasate.
    Authors: Chou M, Burnouf T, Wang T
    PLoS ONE, 2014;9(6):e99145.
    Species: Human
    Sample Type: Cell Culture Supernates
  15. The evolution of cellular deficiency in GATA2 mutation.
    Authors: Dickinson R, Milne P, Jardine L, Zandi S, Swierczek S, McGovern N, Cookson S, Ferozepurwalla Z, Langridge A, Pagan S, Gennery A, Heiskanen-Kosma T, Hamalainen S, Seppanen M, Helbert M, Tholouli E, Gambineri E, Reykdal S, Gottfreethsson M, Thaventhiran J, Morris E, Hirschfield G, Richter A, Jolles S, Bacon C, Hambleton S, Haniffa M, Bryceson Y, Allen C, Prchal J, Dick J, Bigley V, Collin M
    Blood, 2014;123(6):863-74.
    Species: Human
    Sample Type: Serum
  16. Plasma biomarkers as predictors of outcome in patients with advanced hepatocellular carcinoma.
    Authors: Llovet JM, Pena CE, Lathia CD, Shan M, Meinhardt G, Bruix J, Pallota MG, Zarba JJ, Boyer M, Riordan S, Strickland A, Tebbutt N, Thomson B, Borbath I, De Greve J, Van Laethem J-, Van Steenbergen W, Van Vlierberghe H, Barrios C, Cosme de Oliveira A, Kotzev I, Takov D, Tchernev K, Burak K, Ma M, Metrakos P, Olweny C, Sherman M, Gamargo Garate C, Martinez-Castillo J, Beaugrand M, Bennouna J, Blanc J-, Bronowicki J-, Degos F, Dominguez S, Grange J-, Hillon P, Raoul J-, Seitz J-, Blum H, Buggisch P, Caspary W, Dollinger M, Gerken G, Goke B, Gregor M, Greten T, Haussinger D, Hilgard P, Scherübl J, Scheulen M, Schmid R, Spengler U, Wiest R, Zeuzem S, Arvanitakis C, Germanidis G, Katsos I, Figer A, Stemmer S, Amadori D, Bolondi L, Cognetti F, Craxi A, Farinati F, Gridelli C, Martoni A, Mazzaferro V, Porta C, Ricci S, Sangiovanni A, Santoro A, Trevisani F, Cisnero Garza LE, Gane E, O'Donnell A, Leon J, Lozano A, Jassem J, Rydzewska G, Szawlowski A, Tomczak P, Badulescu F, Miron L, Kubyshkin V, Bruix J, Forner A, Bustamante Schneider J, Diago M, Montero Alvarez JL, Pascual S, Ruíz del Arbol L, Sangro B, Solá R, Tabernero J, Muellhaupt B, Roth A, Jeffry Evans TR, Falk S, Meyer T, Reeves H, Ross P, Befeler A, Boyer T, Britten C, Byrne T, Garcia-Tsao G, Gold P, Goldenberg A, Heuman D, Kennedy P, Koch A, Llovet JM, Marrero J, Schilsky M, Schwartz J, Schwartz M
    Clin. Cancer Res., 2012;18(8):2290-300.
    Species: Human
    Sample Type: Plasma
  17. Decreased plasma cytokines are associated with low platelet counts in aplastic anemia and immune thrombocytopenic purpura.
    J. Thromb. Haemost., 2012;10(8):1616-23.
    Species: Human
    Sample Type: Serum
  18. Activation of HER family signaling as a mechanism of acquired resistance to ALK inhibitors in EML4-ALK-positive non-small cell lung cancer.
    Authors: Tanizaki J, Okamoto I, Okabe T, Sakai K, Tanaka K, Hayashi H, Kaneda H, Takezawa K, Kuwata K, Yamaguchi H, Hatashita E, Nishio K, Nakagawa K
    Clin Cancer Res, 2012;18(22):6219-26.
    Species: Human
    Sample Type: Cell Culture Supernates
  19. Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans.
    Authors: Aronis KN, Diakopoulos KN, Fiorenza CG
    Diabetologia, 2011;54(9):2358-67.
    Species: Human
    Sample Type: Serum
  20. Expression and prognostic value of circulating angiogenic cytokines in pancreatic cancer.
    Authors: Rahbari NN, Schmidt T, Falk CS, Hinz U, Herber M, Bork U, Buchler MW, Weitz J, Koch M
    BMC Cancer, 2011;11(0):286.
    Species: Human
    Sample Type: Serum
  21. Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
    Authors: Rauch C, Feifel E, Amann EM, Spotl HP, Schennach H, Pfaller W, Gstraunthaler G
    ALTEX, 2011;28(4):305-16.
    Species: Human
    Sample Type: Cell Lysates
  22. Tumor-derived pancreatic stellate cells promote pancreatic cancer cell invasion through release of thrombospondin-2.
    Authors: Farrow B, Berger DH, Rowley D
    J. Surg. Res., 2009;156(1):155-60.
    Species: Human
    Sample Type: Cell Culture Supernates
  23. Molecular response to cetuximab and efficacy of preoperative cetuximab-based chemoradiation in rectal cancer.
    Authors: Debucquoy A, Haustermans K, Daemen A, Aydin S, Libbrecht L, Gevaert O, De Moor B, Tejpar S, McBride WH, Penninckx F, Scalliet P, Stroh C, Vlassak S, Sempoux C, Machiels JP
    J. Clin. Oncol., 2009;27(17):2751-7.
    Species: Human
    Sample Type: Plasma
  24. Vascular endothelial growth factor drives autocrine epithelial cell proliferation and survival in chronic rhinosinusitis with nasal polyposis.
    Authors: Lee HS, Myers A, Kim J
    Am. J. Respir. Crit. Care Med., 2009;180(11):1056-67.
    Species: Human
    Sample Type: Nasal Lavage
  25. Effect of protein kinase Cbeta inhibition on renal hemodynamic function and urinary biomarkers in humans with type 1 diabetes: a pilot study.
    Authors: Cherney DZ, Konvalinka A, Zinman B, Diamandis EP, Soosaipillai A, Reich H, Lorraine J, Lai V, Scholey JW, Miller JA
    Diabetes Care, 2009;32(1):91-3.
    Species: Human
    Sample Type: Urine
  26. A urinary biomarker profile for children with HIV-associated renal diseases.
    Authors: Soler-Garcia AA, Rakhmanina NY, Mattison PC, Ray PE
    Kidney Int., 2009;76(2):207-14.
    Species: Human
    Sample Type: Urine
  27. Specific inhibition of tissue kallikrein 1 with a human monoclonal antibody reveals a potential role in airway diseases.
    Authors: Sexton DJ, Chen T, Martik D, Kuzmic P, Kuang G, Chen J, Nixon AE, Zuraw BL, Forteza RM, Abraham WM, Wood CR
    Biochem. J., 2009;422(2):383-92.
    Species: Human
    Sample Type: Cell Culture Supernates
  28. Phase II study and biomarker analysis of cetuximab combined with modified FOLFOX6 in advanced gastric cancer.
    Authors: Han SW, Oh DY, Im SA, Park SR, Lee KW, Song HS, Lee NS, Lee KH, Choi IS, Lee MH, Kim MA, Kim WH, Bang YJ, Kim TY
    Br. J. Cancer, 2009;100(2):298-304.
    Species: Human
    Sample Type: Serum
  29. Epidermal Growth Factor Receptor Blockade in Combination with Conventional Chemotherapy Inhibits Soft Tissue Sarcoma Cell Growth In vitro and In vivo.
    Authors: Ren W, Korchin B, Zhu QS, Wei C, Dicker A, Heymach J, Lazar A, Pollock RE, Lev D
    Clin. Cancer Res., 2008;14(9):2785-2795.
    Species: Human
    Sample Type: Cell Culture Supernates
  30. Effective inhibition of the epidermal growth factor/epidermal growth factor receptor binding by anti-epidermal growth factor antibodies is related to better survival in advanced non-small-cell lung cancer patients treated with the epidermal growth factor cancer vaccine.
    Authors: Garcia B, Neninger E, de la Torre A, Leonard I, Martinez R, Viada C, Gonzalez G, Mazorra Z, Lage A, Crombet T
    Clin. Cancer Res., 2008;14(3):840-6.
    Species: Human
    Sample Type: Serum
  31. Human bone marrow activates the Akt pathway in metastatic prostate cells through transactivation of the alpha-platelet-derived growth factor receptor.
    Authors: Dolloff NG, Russell MR, Loizos N, Fatatis A
    Cancer Res., 2007;67(2):555-62.
    Species: Human
    Sample Type: Cell Culture Supernates
  32. Alteration of the serum levels of the epidermal growth factor receptor and its ligands in patients with non-small cell lung cancer and head and neck carcinoma.
    Authors: Lemos-Gonzalez Y, Rodriguez-Berrocal FJ, Cordero OJ, Gomez C, Paez de la Cadena M
    Br. J. Cancer, 2007;96(10):1569-78.
    Species: Human
    Sample Type: Serum
  33. The levels of steroid hormones and cytokines in individual follicles are not associated with the fertilization outcome after intracytoplasmic sperm injection.
    Authors: Asimakopoulos B, Abu-Hassan D, Metzen E, Al-Hasani S, Diedrich K, Nikolettos N
    Fertil. Steril., 2007;90(1):60-4.
    Species: Human
    Sample Type: Follicular Fluid
  34. Selective cyclooxygenase-2 inhibitor downregulates the paracrine epithelial-mesenchymal interactions of growth in scirrhous gastric carcinoma.
    Authors: Yashiro M, Nakazawa K, Tendo M, Kosaka K, Shinto O, Hirakawa K
    Int. J. Cancer, 2007;120(3):686-93.
    Species: Human
    Sample Type: Cell Culture Supernates
  35. Cytokine profiling in human colostrum and milk by protein array.
    Authors: Kverka M, Burianova J, Lodinova-Zadnikova R, Kocourkova I, Cinova J, Tuckova L, Tlaskalova-Hogenova H
    Clin. Chem., 2007;53(5):955-62.
    Species: Human
    Sample Type: Milk
  36. Salivary leptin induces increased expression of growth factors in oral keratinocytes.
    Authors: Groschl M, Topf HG, Kratzsch J, Dotsch J, Rascher W, Rauh M
    J. Mol. Endocrinol., 2005;34(2):353-66.
    Species: Human
    Sample Type: Cell Culture Supernates
  37. Salivary epidermal growth factor in oral cavity cancer.
    Authors: Balicki R, Grabowska SZ, Citko A
    Oral Oncol., 2005;41(1):48-55.
    Species: Human
    Sample Type: Saliva
  38. Factors associated with the outcomes of children with unilateral ureteropelvic junction obstruction.
    Authors: Chiou YY, Chiu NT, Wang ST, Cheng HL, Tang MJ
    J. Urol., 2004;171(1):397-402; disc.
    Species: Human
    Sample Type: Urine
  39. Alterations in antioxidant power and levels of epidermal growth factor and nitric oxide in saliva of patients with inflammatory bowel diseases.
    Authors: Jahanshahi G, Motavasel V, Rezaie A, Hashtroudi AA, Daryani NE, Abdollahi M
    Dig. Dis. Sci., 2004;49(11):1752-7.
    Species: Human
    Sample Type: Plasma
  40. A potential pro-angiogenic cell therapy with human placenta-derived mesenchymal cells.
    Authors: Nishishita T, Ouchi K, Zhang X, Inoue M, Inazawa T, Yoshiura K, Kuwabara K, Nakaoka T, Watanabe N, Igura K, Takahashi TA, Yamashita N
    Biochem. Biophys. Res. Commun., 2004;325(1):24-31.
    Species: Human
    Sample Type: Cell Lysates
  41. AF5, a CNS cell line immortalized with an N-terminal fragment of SV40 large T: growth, differentiation, genetic stability, and gene expression.
    Authors: Truckenmiller ME, Vawter MP, Zhang P, Conejero-Goldberg C, Dillon-Carter O, Morales N, Cheadle C, Becker KG, Freed WJ
    Exp. Neurol., 2002;175(0):318.
    Species: Rat
    Sample Type: Cell Culture Supernates
  42. The significance of cytokines, chemical composition, and murine embryo development in hydrosalpinx fluid for predicting the IVF outcome in women with hydrosalpinx.
    Authors: Chen CD, Yang JH, Lin KC, Chao KH, Ho HN, Yang YS
    Hum. Reprod., 2002;17(1):128-33.
    Species: Human
    Sample Type: Hydrosalpinx Fluid
  43. Saliva analysis in the clinical setting: revisiting an underused diagnostic tool.
    Authors: Nagler RM, Hershkovich O, Lischinsky S, Diamond E, Reznick AZ
    J. Investig. Med., 2002;50(3):214-25.
    Species: Human
    Sample Type: Saliva
  44. Epithelial cells in nasal fluids from patients with allergic rhinitis: how do they relate to epidermal growth factor, eosinophils and eosinophil cationic protein?
    Authors: Benson M, Uddman R, Cardell LO
    Acta Otolaryngol., 2002;122(2):202-5.
    Species: Human
    Sample Type: Nasal Lavage
  45. Diminished epidermal growth factor levels in infants with necrotizing enterocolitis.
    Authors: Shin CE, Falcone RA, Stuart L, Erwin CR, Warner BW
    J. Pediatr. Surg., 2000;35(2):173-6; discus.
    Species: Human
    Sample Type: Serum
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