Human EGF Quantikine ELISA Kit

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Human EGF ELISA Cell Culture Supernate/Urine Standard Curve
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Human EGF Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (10 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Platelet-poor Citrate Plasma (200 uL), Urine (10 uL)
Sensitivity
0.7 pg/mL
Assay Range
3.9 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma, Urine)
Specificity
Natural and recombinant human EGF
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Plasma/Saliva/Urine - Samples from apparently healthy volunteers were evaluated for the presence of human EGF in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean of Detectable (pg/mL)% DetectableRange (pg/mL)
Platelet-poor EDTA plasma (n=37)4.8754.54-5.20
Platelet-poor heparin plasma (n=37)4.8116ND-5.43
Platelet-poor citrate plasma (n=37)ND0_____
ND=Non-detectable

Sample TypeMean (pg/mL)Range (pg/mL)Standard Deviation (pg/mL)
Serum (n=37)409106-1063151
Saliva (n=14)170381.8-96002400

Sample TypeMean (μg/g Creatinine)Range (μg/g Creatinine)Standard Deviation (μg/g Creatinine)
Urine (n=11)23,01912,352-36,8948524

Cell Culture Supernates - - Human peripheral blood leukocytes (PBL; 1 x 106 cells) were cultured in RPMI 1640 and supplemented with 10% fetal bovine serum. Cells were cultured stimulated with 10 μg/mL PHA for 6 days. An aliquot of the cell culture supernate was removed, assayed for human EGF, and measured 5.4 pg/mL.


Product Summary

The Quantikine Human EGF Immunoassay kit is a 3.5 or 4.5 hour solid phase ELISA designed to measure EGF levels in cell culture supernates, serum, plasma, and urine. It contains E. coli-derived recombinant human EGF and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human EGF. Results obtained measuring natural human EGF showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural EGF.

Recovery

The recovery of EGF spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 98 88-111
Citrate Plasma (n=10) 104 89-115
EDTA Plasma (n=10) 100 80-111
Heparin Plasma (n=10) 96 79-108
Serum (n=10) 97 80-106
Urine (n=1) 102 97-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of EGF in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human EGF ELISA Linearity

Scientific Data

Human EGF ELISA Cell Culture Supernate/Urine Standard Curve

Human EGF ELISA Serum/Plasma/Saliva Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: EGF

EGF (Epidermal Growth Factor) is expressed as a transmembrane protein that is proteolytically cleaved to generate soluble forms. It signals through EGF R/ErbB1 which heterodimerizes with ErbB2, ErbB3, or ErbB4 and can also associate with PDGF R beta and HGF R/c-MET. During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult, EGF plays a role in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration.

Long Name:
Epidermal Growth Factor
Entrez Gene IDs:
1950 (Human); 13645 (Mouse); 25313 (Rat)
Alternate Names:
beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; hEGF; HOMG4; pro-epidermal growth factor; URG; Urogastrone
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent for Serum & Plasma Samples Only
  4.   For Serum & Plasma Samples Only: Add 50 µL of Assay Diluent to each well.

  5. 200 µL Standard, Control, or Sample
  6.   Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well.
  10.   For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    For Cell Culture Supernate & Urine Samples: Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  11.   Aspirate and wash 3 times.

  12. 200 µL Substrate Solution
  13.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

  14. 50 µL Stop Solution
  15. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human EGF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

76 Citations: Showing 1 - 10
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  1. Urinary epidermal growth factor levels correlate with cardiovascular autonomic neuropathy indices in adults with type 1 diabetes
    Authors: Lin, YK;Tanner, E;Wang, Y;Ye, W;Ang, L;Ju, W;Pop-Busui, R;
    Journal of diabetes investigation
    Species: Human
    Sample Types: Urine
  2. Inhibition of oxidative stress-induced epithelial-mesenchymal transition in retinal pigment epithelial cells of age-related macular degeneration model by suppressing ERK activation
    Authors: Yang, YC;Chien, Y;Yarmishyn, AA;Lim, LY;Tsai, HY;Kuo, WC;Tsai, PH;Yang, SH;Hong, SI;Chen, SJ;Hwang, DK;Yang, YP;Chiou, SH;
    Journal of advanced research
    Species: Human
    Sample Types: Cell Culture Supernates
  3. TRPV1 inhibition overcomes cisplatin resistance by blocking autophagy-mediated hyperactivation of EGFR signaling pathway
    Authors: Oh, SJ;Lim, JY;Son, MK;Ahn, JH;Song, KH;Lee, HJ;Kim, S;Cho, EH;Chung, JY;Cho, H;Kim, H;Kim, JH;Park, J;Choi, J;Hwang, SW;Kim, TW;
    Nature communications
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Crystalline silica-exposed human lung epithelial cells presented enhanced anchorage-independent growth with upregulated expression of BRD4 and EZH2 in autocrine and paracrine manners
    Authors: Katabami, M;Kinoshita, I;Ariga, S;Shimizu, Y;Dosaka-Akita, H;
    PloS one
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Impact of Freeze-Drying on Cord Blood (CB), Serum (S), and Platelet-Rich Plasma (CB-PRP) Preparations on Growth Factor Content and In Vitro Cell Wound Healing
    Authors: V Sabrina, C Carmen, A Gloria, B Elisa, C Nico, B Marina, F Luigi, V Piera
    International Journal of Molecular Sciences, 2022-09-14;23(18):.
    Species: Human
    Sample Types: Serum
  6. Predicting treatment response and clinicopathological findings in lupus nephritis with urine epidermal growth factor, monocyte chemoattractant protein-1 or their ratios
    Authors: P Ngamjanyap, S Worawichaw, P Pisitkun, K Khiewngam, S Kantachuve, A Nongnuch, M Assanatham, N Sathirapon, C Kitiyakara
    PLoS ONE, 2022-03-10;17(3):e0263778.
    Species: Human
    Sample Types: Urine
  7. A comparative study of pathogen inactivation technologies in human platelet lysate and its optimal efficiency in human placenta-derived stem cells culture
    Authors: E Razani, HR Aghayan, S Alavi-Mogh, F Yari, A Gharehbagh, Z Sharifi
    Journal of virological methods, 2022-01-29;0(0):114478.
    Species: Human
    Sample Types: Cell Lysates
  8. Oncogenic KRAS promotes growth of lung cancer cells expressing SLC3A2-NRG1 fusion via ADAM17-mediated shedding of NRG1
    Authors: DH Shin, SH Kim, M Choi, YK Bae, C Han, BK Choi, SS Kim, JY Han
    Oncogene, 2021-11-06;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Circulating cytokines present in multiple myeloma patients inhibit the osteoblastic differentiation of adipose stem cells
    Authors: L Kobari, M Auclair, O Piau, N Ferrand, M Zaoui, F Delhommeau, B Fève, M Sabbah, L Garderet
    Leukemia, 2021-09-23;0(0):.
    Species: Human
    Sample Types: Plasma
  10. Combination of Epithelial Growth Factor Receptor Blockers and CDK4/6 Inhibitor for Nasopharyngeal Carcinoma Treatment
    Authors: HP Li, CY Huang, KW Lui, YK Chao, CN Yeh, LY Lee, Y Huang, TL Lin, YC Kuo, MY Huang, YR Lai, YM Yeh, HC Fan, AC Lin, JC Hsieh, KP Chang, CY Lin, HM Wang, YS Chang, CL Hsu
    Cancers, 2021-06-12;13(12):.
    Species: Human
    Sample Types: Plasma
  11. NRF2 Enables EGFR Signaling in Melanoma Cells
    Authors: JKC Kre beta, C Jessen, A Marquardt, A Hufnagel, S Meierjohan
    International Journal of Molecular Sciences, 2021-04-07;22(8):.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Enhanced MCP-1 Release in Early Autosomal Dominant Polycystic Kidney Disease
    Authors: P Janssens, JP Decuypere, S De Rechter, L Breysem, D Van Giel, J Billen, A Hindryckx, L De Catte, M Baldewijns, KBM Claes, KM Wissing, K Devriendt, B Bammens, I Meyts, VE Torres, R Vennekens, D Mekahli
    Kidney international reports, 2021-04-06;6(6):1687-1698.
    Species: Human
    Sample Types: Urine
  13. Probiotic-Derived Polyphosphate Accelerates Intestinal Epithelia Wound Healing through Inducing Platelet-Derived Mediators
    Authors: S Isozaki, H Konishi, M Fujiya, H Tanaka, Y Murakami, S Kashima, K Ando, N Ueno, K Moriichi, T Okumura
    Mediators of Inflammation, 2021-03-29;2021(0):5582943.
    Species: Human
    Sample Types: Plasma
  14. In Ovarian Cancer Multicellular Spheroids, Platelet Releasate Promotes Growth, Expansion of ALDH+ and CD133+ Cancer Stem Cells, and Protection against the Cytotoxic Effects of Cisplatin, Carboplatin and Paclitaxel
    Authors: N Casagrande, C Borghese, F Agostini, C Durante, M Mazzucato, A Colombatti, D Aldinucci
    International Journal of Molecular Sciences, 2021-03-16;22(6):.
    Species: Human
    Sample Types: Plasma
  15. Expression, Purification and Functional Identification of the Modified hEGF Protein
    Authors: H Guo, F Hu, K Li, Z Zhao, Z Li, X Xu, X Gou
    Protein Expr Purif, 2020-11-14;179(0):105787.
    Species: Human
    Sample Types: Tissue Homogenates
  16. Anti-epidermal growth factor vaccine antibodies increase the antitumor activity of kinase inhibitors in ALK and RET rearranged lung cancer cells
    Authors: J Codony-Ser, S García-Rom, MÁ Molina-Vil, J Bertran-Al, S Viteri, E d'Hondt, R Rosell
    Transl Oncol, 2020-10-23;14(1):100887.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. LC3B upregulation by NANOG promotes immune resistance and stem-like property through hyperactivation of EGFR signaling in immune-refractory tumor cells
    Authors: S Kim, H Cho, SO Hong, SJ Oh, HJ Lee, E Cho, SR Woo, JS Song, JY Chung, SW Son, SM Yoon, YM Jeon, S Jeon, C Yee, KM Lee, SM Hewitt, JH Kim, KH Song, TW Kim
    Autophagy, 2020-08-14;0(0):1-20.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Repeated Freezing Procedures Preserve Structural and Functional Properties of Amniotic Membrane for Application in Ophthalmology
    Authors: O Pogozhykh, N Hofmann, O Gryshkov, C von Kaisen, M Mueller, B Glasmacher, D Pogozhykh, M Börgel, R Blasczyk, C Figueiredo
    Int J Mol Sci, 2020-06-04;21(11):.
    Species: Human
    Sample Types: Tissue Homogenates
  19. Anti-EGFR Therapy Induces EGF Secretion by Cancer-Associated Fibroblasts to Confer Colorectal Cancer Chemoresistance
    Authors: CM Garvey, R Lau, A Sanchez, RX Sun, EJ Fong, ME Doche, O Chen, A Jusuf, HJ Lenz, B Larson, SM Mumenthale
    Cancers (Basel), 2020-05-28;12(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Cord Blood Serum (CBS)-Based Eye Drops Modulate Light-Induced Neurodegeneration in Albino Rat Retinas
    Authors: S Di Marco, S Riccitelli, M Di Paolo, E Campos, M Buzzi, S Bisti, P Versura
    Biomolecules, 2020-04-28;10(5):.
    Species: Human
    Sample Types: Cord Blood Serum
  21. MYST1/KAT8 contributes to tumor progression by activating EGFR signaling in glioblastoma cells
    Authors: Z Dong, J Zou, J Li, Y Pang, Y Liu, C Deng, F Chen, H Cui
    Cancer Med, 2019-11-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
  22. CD206+ tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production
    Authors: ASMR Haque, M Moriyama, K Kubota, N Ishiguro, M Sakamoto, A Chinju, K Mochizuki, T Sakamoto, N Kaneko, R Munemura, T Maehara, A Tanaka, JN Hayashida, S Kawano, T Kiyoshima, S Nakamura
    Sci Rep, 2019-10-10;9(1):14611.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Ginsenoside Rg1 Accelerates Paracrine Activity and Adipogenic Differentiation of Human Breast Adipose-Derived Stem Cells in a Dose-Dependent Manner In Vitro
    Authors: ZJ Liang, X Lu, DD Zhu, XL Yi, FX Wu, N He, C Tang, CY Wei, HM Li
    Cell Transplant, 2019-01-24;0(0):9636897198256.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Urinary epidermal growth factor, monocyte chemoattractant protein-1 or their ratio as predictors for rapid loss of renal function in type 2 diabetic patients with diabetic kidney disease
    Authors: B Satirapoj, R Dispan, P Radinahame, C Kitiyakara
    BMC Nephrol, 2018-09-21;19(1):246.
    Species: Human
    Sample Types: Urine
  25. 1H-NMR and MALDI-TOF MS as metabolomic quality control tests to classify platelet derived medium additives for GMP compliant cell expansion procedures
    Authors: F Agostini, M Ruzza, D Corpillo, L Biondi, E Acquadro, B Canepa, A Viale, M Battiston, F Serra, S Aime, M Mazzucato
    PLoS ONE, 2018-09-06;13(9):e0203048.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Human serum and platelet lysate are appropriate xeno-free alternatives for clinical-grade production of human MuStem cell batches
    Authors: C Saury, A Lardenois, C Schleder, I Leroux, B Lieubeau, L David, M Charrier, L Guével, S Viau, B Delorme, K Rouger
    Stem Cell Res Ther, 2018-05-02;9(1):128.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Therapeutic cancer vaccine: phase I clinical tolerance study of Hu-rhEGF-rP64k/Mont in patients with newly diagnosed advanced non-small cell lung cancer
    Authors: P Xing, H Wang, S Yang, X Han, Y Sun, Y Shi
    BMC Immunol., 2018-04-16;19(1):14.
    Species: Human
    Sample Types: Serum
  28. Deterioration in saliva quality in patients with Sj�gren's syndrome: impact of decrease in salivary epidermal growth factor on the severity of intraoral manifestations
    Authors: N Azuma, Y Katada, H Sano
    Inflamm Regen, 2018-04-09;38(0):6.
    Species: Human
    Sample Types: Saliva
  29. Comparative analyses of industrial-scale human platelet lysate preparations.
    Authors: Jan Pierce, Eric Benedetti, Amber Preslar, Pam Jacobson, Ping Jin, David F Stroncek, Jo-Anna Reems
    Transfusion, 2017-10-08;0(0):1537-2995.
    Species: Human
    Sample Types: Plasma
  30. Umbilical cord extracts improve diabetic abnormalities in bone marrow-derived mesenchymal stem cells and increase their therapeutic effects on diabetic nephropathy
    Authors: K Nagaishi, Y Mizue, T Chikenji, M Otani, M Nakano, Y Saijo, H Tsuchida, S Ishioka, A Nishikawa, T Saito, M Fujimiya
    Sci Rep, 2017-08-16;7(1):8484.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Differential effects of amnion and chorion membrane extracts on osteoblast-like cells due to the different growth factor composition of the extracts
    Authors: YY Go, SE Kim, GJ Cho, SW Chae, JJ Song
    PLoS ONE, 2017-08-10;12(8):e0182716.
    Species: Human
    Sample Types: Tissue Homogenates
  32. Design, characterization and experimental validation of a compact, flexible pulsed power architecture for ex vivo platelet activation
    Authors: AL Garner, A Caiafa, Y Jiang, S Klopman, C Morton, AS Torres, AM Loveless, VB Neculaes
    PLoS ONE, 2017-07-26;12(7):e0181214.
    Species: Human
    Sample Types: Plasma
  33. The p38 signaling pathway mediates quiescence of glioma stem cells by regulating epidermal growth factor receptor trafficking
    Authors: A Soeda, J Lathia, BJ Williams, Q Wu, J Gallagher, A Androutsel, AJ Giles, C Yang, Z Zhuang, MR Gilbert, JN Rich, DM Park
    Oncotarget, 2017-05-16;8(20):33316-33328.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Inflammatory Differences in Plaque Erosion and Rupture in Patients With ST-Segment Elevation Myocardial Infarction
    Authors: S Chandran, J Watkins, A Abdul-Aziz, M Shafat, PA Calvert, KM Bowles, MD Flather, SA Rushworth, AD Ryding
    J Am Heart Assoc, 2017-05-03;6(5):.
    Species: Human
    Sample Types: Plasma
  35. The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro
    Authors: M Bernardi, F Agostini, K Chieregato, E Amati, C Durante, M Rassu, M Ruggeri, S Sella, E Lombardi, M Mazzucato, G Astori
    J Transl Med, 2017-05-01;15(1):90.
    Species: Human
    Sample Types: Cell Lysates
  36. C-X-C motif chemokine ligand 8 promotes endothelial cell homing via the Akt-signal transducer and activator of transcription pathway to accelerate healing of ischemic and hypoxic skin ulcers
    Authors: L Shen, P Zhang, S Zhang, L Xie, L Yao, W Lang, J Lian, W Qin, M Zhang, L Ji
    Exp Ther Med, 2017-04-05;13(6):3021-3031.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. Urine Epidermal Growth Factor, Monocyte Chemoattractant Protein-1 or Their Ratio as Biomarkers for Interstitial Fibrosis and Tubular Atrophy in Primary Glomerulonephritis
    Authors: Supanat Worawichaw
    Kidney Blood Press. Res, 2016-12-19;41(6):997-1007.
    Species: Human
    Sample Types: Urine
  38. Overcoming the bottleneck of platelet lysate supply in large-scale clinical expansion of adipose-derived stem cells: A comparison of fresh versus three types of platelet lysates from outdated buffy coat-derived platelet concentrates
    Authors: Anne Fischer-Ni
    Cytotherapy, 2016-11-22;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. Biological effects of plasma rich in growth factors (PRGF) on human endometrial fibroblasts
    Authors: Gorka Orive
    Eur. J. Obstet. Gynecol. Reprod. Biol., 2016-09-22;206(0):125-130.
    Species: Human
    Sample Types: Plasma
  40. N-Glycosylation of integrin ?5 acts as a switch for EGFR-mediated complex formation of integrin ?5?1 to ?6?4
    Sci Rep, 2016-09-19;6(0):33507.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Adipose-derived mesenchymal stem cells from patients with atherosclerotic renovascular disease have increased DNA damage and reduced angiogenesis that can be modified by hypoxia
    Stem Cell Res Ther, 2016-09-09;7(1):128.
    Species: Human
    Sample Types: Cell Culture Supernates
  42. Serum Interleukin-18, Fetuin-A, Soluble Intercellular Adhesion Molecule-1, and Endothelin-1 in Ankylosing Spondylitis, Psoriatic Arthritis, and SAPHO Syndrome
    Int J Mol Sci, 2016-08-03;17(8):.
    Species: Human
    Sample Types: Serum
  43. Transgenic Soybean Production of Bioactive Human Epidermal Growth Factor (EGF)
    Authors: Yonghua He
    PLoS ONE, 2016-06-17;11(6):e0157034.
    Species: Plant - Arabidopsis
    Sample Types: Protein
  44. Ex vivo expansion of bovine corneal endothelial cells in xeno-free medium supplemented with platelet releasate.
    Authors: Chou M, Burnouf T, Wang T
    PLoS ONE, 2014-06-19;9(6):e99145.
    Species: Human
    Sample Types: Cell Culture Supernates
  45. Endothelial cell-secreted EGF induces epithelial to mesenchymal transition and endows head and neck cancer cells with stem-like phenotype.
    Authors: Zhang Z, Dong Z, Lauxen I, Filho M, Nor J
    Cancer Res, 2014-03-31;74(10):2869-81.
    Species: Human
    Sample Types: Cell Culture Supernates
  46. The evolution of cellular deficiency in GATA2 mutation.
    Authors: Dickinson R, Milne P, Jardine L, Zandi S, Swierczek S, McGovern N, Cookson S, Ferozepurwalla Z, Langridge A, Pagan S, Gennery A, Heiskanen-Kosma T, Hamalainen S, Seppanen M, Helbert M, Tholouli E, Gambineri E, Reykdal S, Gottfreethsson M, Thaventhiran J, Morris E, Hirschfield G, Richter A, Jolles S, Bacon C, Hambleton S, Haniffa M, Bryceson Y, Allen C, Prchal J, Dick J, Bigley V, Collin M
    Blood, 2013-12-17;123(6):863-74.
    Species: Human
    Sample Types: Serum
  47. Decreased plasma cytokines are associated with low platelet counts in aplastic anemia and immune thrombocytopenic purpura.
    J. Thromb. Haemost., 2012-08-01;10(8):1616-23.
    Species: Human
    Sample Types: Serum
  48. Activation of HER family signaling as a mechanism of acquired resistance to ALK inhibitors in EML4-ALK-positive non-small cell lung cancer.
    Authors: Tanizaki J, Okamoto I, Okabe T, Sakai K, Tanaka K, Hayashi H, Kaneda H, Takezawa K, Kuwata K, Yamaguchi H, Hatashita E, Nishio K, Nakagawa K
    Clin Cancer Res, 2012-07-27;18(22):6219-26.
    Species: Human
    Sample Types: Cell Culture Supernates
  49. Plasma biomarkers as predictors of outcome in patients with advanced hepatocellular carcinoma.
    Authors: Llovet JM, Pena CE, Lathia CD, Shan M, Meinhardt G, Bruix J, Pallota MG, Zarba JJ, Boyer M, Riordan S, Strickland A, Tebbutt N, Thomson B, Borbath I, De Greve J, Van Laethem J-, Van Steenbergen W, Van Vlierberghe H, Barrios C, Cosme de Oliveira A, Kotzev I, Takov D, Tchernev K, Burak K, Ma M, Metrakos P, Olweny C, Sherman M, Gamargo Garate C, Martinez-Castillo J, Beaugrand M, Bennouna J, Blanc J-, Bronowicki J-, Degos F, Dominguez S, Grange J-, Hillon P, Raoul J-, Seitz J-, Blum H, Buggisch P, Caspary W, Dollinger M, Gerken G, Goke B, Gregor M, Greten T, Haussinger D, Hilgard P, Scherübl J, Scheulen M, Schmid R, Spengler U, Wiest R, Zeuzem S, Arvanitakis C, Germanidis G, Katsos I, Figer A, Stemmer S, Amadori D, Bolondi L, Cognetti F, Craxi A, Farinati F, Gridelli C, Martoni A, Mazzaferro V, Porta C, Ricci S, Sangiovanni A, Santoro A, Trevisani F, Cisnero Garza LE, Gane E, O'Donnell A, Leon J, Lozano A, Jassem J, Rydzewska G, Szawlowski A, Tomczak P, Badulescu F, Miron L, Kubyshkin V, Bruix J, Forner A, Bustamante Schneider J, Diago M, Montero Alvarez JL, Pascual S, Ruíz del Arbol L, Sangro B, Solá R, Tabernero J, Muellhaupt B, Roth A, Jeffry Evans TR, Falk S, Meyer T, Reeves H, Ross P, Befeler A, Boyer T, Britten C, Byrne T, Garcia-Tsao G, Gold P, Goldenberg A, Heuman D, Kennedy P, Koch A, Llovet JM, Marrero J, Schilsky M, Schwartz J, Schwartz M
    Clin. Cancer Res., 2012-02-28;18(8):2290-300.
    Species: Human
    Sample Types: Plasma
  50. Expression and prognostic value of circulating angiogenic cytokines in pancreatic cancer.
    Authors: Rahbari NN, Schmidt T, Falk CS, Hinz U, Herber M, Bork U, Buchler MW, Weitz J, Koch M
    BMC Cancer, 2011-07-05;11(0):286.
    Species: Human
    Sample Types: Serum
  51. Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans.
    Authors: Aronis KN, Diakopoulos KN, Fiorenza CG
    Diabetologia, 2011-06-10;54(9):2358-67.
    Species: Human
    Sample Types: Serum
  52. Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
    Authors: Rauch C, Feifel E, Amann EM, Spotl HP, Schennach H, Pfaller W, Gstraunthaler G
    ALTEX, 2011-01-01;28(4):305-16.
    Species: Human
    Sample Types: Cell Lysates
  53. Vascular endothelial growth factor drives autocrine epithelial cell proliferation and survival in chronic rhinosinusitis with nasal polyposis.
    Authors: Lee HS, Myers A, Kim J
    Am. J. Respir. Crit. Care Med., 2009-09-17;180(11):1056-67.
    Species: Human
    Sample Types: Nasal Lavage Fluid
  54. Specific inhibition of tissue kallikrein 1 with a human monoclonal antibody reveals a potential role in airway diseases.
    Authors: Sexton DJ, Chen T, Martik D, Kuzmic P, Kuang G, Chen J, Nixon AE, Zuraw BL, Forteza RM, Abraham WM, Wood CR
    Biochem. J., 2009-08-13;422(2):383-92.
    Species: Human
    Sample Types: Cell Culture Supernates
  55. Tumor-derived pancreatic stellate cells promote pancreatic cancer cell invasion through release of thrombospondin-2.
    Authors: Farrow B, Berger DH, Rowley D
    J. Surg. Res., 2009-05-03;156(1):155-60.
    Species: Human
    Sample Types: Cell Culture Supernates
  56. A urinary biomarker profile for children with HIV-associated renal diseases.
    Authors: Soler-Garcia AA, Rakhmanina NY, Mattison PC, Ray PE
    Kidney Int., 2009-04-08;76(2):207-14.
    Species: Human
    Sample Types: Urine
  57. Molecular response to cetuximab and efficacy of preoperative cetuximab-based chemoradiation in rectal cancer.
    Authors: Debucquoy A, Haustermans K, Daemen A, Aydin S, Libbrecht L, Gevaert O, De Moor B, Tejpar S, McBride WH, Penninckx F, Scalliet P, Stroh C, Vlassak S, Sempoux C, Machiels JP
    J. Clin. Oncol., 2009-03-30;27(17):2751-7.
    Species: Human
    Sample Types: Plasma
  58. Phase II study and biomarker analysis of cetuximab combined with modified FOLFOX6 in advanced gastric cancer.
    Authors: Han SW, Oh DY, Im SA, Park SR, Lee KW, Song HS, Lee NS, Lee KH, Choi IS, Lee MH, Kim MA, Kim WH, Bang YJ, Kim TY
    Br. J. Cancer, 2009-01-06;100(2):298-304.
    Species: Human
    Sample Types: Serum
  59. Effect of protein kinase Cbeta inhibition on renal hemodynamic function and urinary biomarkers in humans with type 1 diabetes: a pilot study.
    Authors: Cherney DZ, Konvalinka A, Zinman B, Diamandis EP, Soosaipillai A, Reich H, Lorraine J, Lai V, Scholey JW, Miller JA
    Diabetes Care, 2008-10-22;32(1):91-3.
    Species: Human
    Sample Types: Urine
  60. Epidermal Growth Factor Receptor Blockade in Combination with Conventional Chemotherapy Inhibits Soft Tissue Sarcoma Cell Growth In vitro and In vivo.
    Authors: Ren W, Korchin B, Zhu QS, Wei C, Dicker A, Heymach J, Lazar A, Pollock RE, Lev D
    Clin. Cancer Res., 2008-05-01;14(9):2785-2795.
    Species: Human
    Sample Types: Cell Culture Supernates
  61. Effective inhibition of the epidermal growth factor/epidermal growth factor receptor binding by anti-epidermal growth factor antibodies is related to better survival in advanced non-small-cell lung cancer patients treated with the epidermal growth factor cancer vaccine.
    Authors: Garcia B, Neninger E, de la Torre A, Leonard I, Martinez R, Viada C, Gonzalez G, Mazorra Z, Lage A, Crombet T
    Clin. Cancer Res., 2008-02-01;14(3):840-6.
    Species: Human
    Sample Types: Serum
  62. The levels of steroid hormones and cytokines in individual follicles are not associated with the fertilization outcome after intracytoplasmic sperm injection.
    Authors: Asimakopoulos B, Abu-Hassan D, Metzen E, Al-Hasani S, Diedrich K, Nikolettos N
    Fertil. Steril., 2007-11-05;90(1):60-4.
    Species: Human
    Sample Types: Follicular Fluid
  63. Alteration of the serum levels of the epidermal growth factor receptor and its ligands in patients with non-small cell lung cancer and head and neck carcinoma.
    Authors: Lemos-Gonzalez Y, Rodriguez-Berrocal FJ, Cordero OJ, Gomez C, Paez de la Cadena M
    Br. J. Cancer, 2007-04-24;96(10):1569-78.
    Species: Human
    Sample Types: Serum
  64. Cytokine profiling in human colostrum and milk by protein array.
    Authors: Kverka M, Burianova J, Lodinova-Zadnikova R, Kocourkova I, Cinova J, Tuckova L, Tlaskalova-Hogenova H
    Clin. Chem., 2007-03-15;53(5):955-62.
    Species: Human
    Sample Types: Milk
  65. Selective cyclooxygenase-2 inhibitor downregulates the paracrine epithelial-mesenchymal interactions of growth in scirrhous gastric carcinoma.
    Authors: Yashiro M, Nakazawa K, Tendo M, Kosaka K, Shinto O, Hirakawa K
    Int. J. Cancer, 2007-02-01;120(3):686-93.
    Species: Human
    Sample Types: Cell Culture Supernates
  66. Human bone marrow activates the Akt pathway in metastatic prostate cells through transactivation of the alpha-platelet-derived growth factor receptor.
    Authors: Dolloff NG, Russell MR, Loizos N, Fatatis A
    Cancer Res., 2007-01-15;67(2):555-62.
    Species: Human
    Sample Types: Cell Culture Supernates
  67. Salivary leptin induces increased expression of growth factors in oral keratinocytes.
    Authors: Groschl M, Topf HG, Kratzsch J, Dotsch J, Rascher W, Rauh M
    J. Mol. Endocrinol., 2005-04-01;34(2):353-66.
    Species: Human
    Sample Types: Cell Culture Supernates
  68. Salivary epidermal growth factor in oral cavity cancer.
    Authors: Balicki R, Grabowska SZ, Citko A
    Oral Oncol., 2005-01-01;41(1):48-55.
    Species: Human
    Sample Types: Saliva
  69. A potential pro-angiogenic cell therapy with human placenta-derived mesenchymal cells.
    Authors: Nishishita T, Ouchi K, Zhang X, Inoue M, Inazawa T, Yoshiura K, Kuwabara K, Nakaoka T, Watanabe N, Igura K, Takahashi TA, Yamashita N
    Biochem. Biophys. Res. Commun., 2004-12-03;325(1):24-31.
    Species: Human
    Sample Types: Cell Lysates
  70. Alterations in antioxidant power and levels of epidermal growth factor and nitric oxide in saliva of patients with inflammatory bowel diseases.
    Authors: Jahanshahi G, Motavasel V, Rezaie A, Hashtroudi AA, Daryani NE, Abdollahi M
    Dig. Dis. Sci., 2004-11-01;49(11):1752-7.
    Species: Human
    Sample Types: Plasma
  71. Factors associated with the outcomes of children with unilateral ureteropelvic junction obstruction.
    Authors: Chiou YY, Chiu NT, Wang ST, Cheng HL, Tang MJ
    J. Urol., 2004-01-01;171(1):397-402; disc.
    Species: Human
    Sample Types: Urine
  72. AF5, a CNS cell line immortalized with an N-terminal fragment of SV40 large T: growth, differentiation, genetic stability, and gene expression.
    Authors: Truckenmiller ME, Vawter MP, Zhang P, Conejero-Goldberg C, Dillon-Carter O, Morales N, Cheadle C, Becker KG, Freed WJ
    Exp. Neurol., 2002-06-01;175(0):318.
    Species: Rat
    Sample Types: Cell Culture Supernates
  73. Saliva analysis in the clinical setting: revisiting an underused diagnostic tool.
    Authors: Nagler RM, 2019, Hershkovich O, Lischinsky S, Diamond E, Reznick AZ
    1333-1343.e7, 2002-05-01;50(3):214-25.
    Species: Human
    Sample Types: Saliva
  74. Epithelial cells in nasal fluids from patients with allergic rhinitis: how do they relate to epidermal growth factor, eosinophils and eosinophil cationic protein?
    Authors: Benson M, Uddman R, Cardell LO
    Acta Otolaryngol., 2002-03-01;122(2):202-5.
    Species: Human
    Sample Types: Nasal Lavage Fluid
  75. The significance of cytokines, chemical composition, and murine embryo development in hydrosalpinx fluid for predicting the IVF outcome in women with hydrosalpinx.
    Authors: Chen CD, Yang JH, Lin KC, Chao KH, Ho HN, Yang YS
    Hum. Reprod., 2002-01-01;17(1):128-33.
    Species: Human
    Sample Types: Hydrosalpinx Fluid
  76. Diminished epidermal growth factor levels in infants with necrotizing enterocolitis.
    Authors: Shin CE, Falcone RA, Stuart L, Erwin CR, Warner BW
    J. Pediatr. Surg., 2000-02-01;35(2):173-6; discus.
    Species: Human
    Sample Types: Serum

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Human EGF Quantikine ELISA Kit
By Anonymous on 03/15/2022
Sample Tested: Plasma,Serum,Urine

We analyzed urine at a 150-fold dilution and plasma/serum at a 10-fold dilution. The standard curve had an excellent 4PL fit. We continue to use this assay routinely.


Human EGF Quantikine ELISA Kit
By Anonymous on 12/11/2021
Sample Tested: Serum

Human EGF Quantikine ELISA Kit
By Anonymous on 09/13/2018
Sample Tested: 293T human embryonic kidney cell line,T47D human breast cancer cell line

Human EGF Quantikine ELISA Kit
By Jeff Pimentel on 06/12/2018
Sample Tested: Serum

Human EGF Quantikine ELISA Kit
By Alisha Freeman on 04/03/2018
Sample Tested: Adult thymocytes