• Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    3.5 hours or 4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (200 uL), Serum (10 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Platelet-poor Citrate Plasma (200 uL), Urine (10 uL)
  • Sensitivity
    0.7 pg/mL
  • Assay Range
    3.9 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma, Urine)
  • Specificity
    Natural and recombinant human EGF
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC21, Quantikine Immunoassay Control Group 4 - Please Inquire
Product Summary
The Quantikine Human EGF Immunoassay kit is a 3.5 or 4.5 hour solid phase ELISA designed to measure EGF levels in cell culture supernates, serum, plasma, and urine. It contains E. coli-derived recombinant human EGF and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human EGF. Results obtained measuring natural human EGF showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural EGF.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean13.58013314.777134
Standard Deviation0.622.70.93.36.1
CV%4.42.526.14.34.6

Cell Culture Supernates, Urine
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean1578.91341580136
Standard Deviation0.42.540.94.56.8
CV%2.73.2365.65

Recovery

The recovery of EGF spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 98 88-111
Citrate Plasma (n=10) 104 89-115
EDTA Plasma (n=10) 100 80-111
Heparin Plasma (n=10) 96 79-108
Serum (n=10) 97 80-106
Urine (n=1) 102 97-108
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of EGF in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human EGF Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: EGF
EGF (Epidermal Growth Factor) is expressed as a transmembrane protein that is proteolytically cleaved to generate soluble forms. It signals through EGF R/ErbB1 which heterodimerizes with ErbB2, ErbB3, or ErbB4 and can also associate with PDGF R beta and HGF R/c-MET. During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult, EGF plays a role in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration.
    • Long Name
      Epidermal Growth Factor
    • Entrez Gene IDs
      1950 (Human); 13645 (Mouse); 25313 (Rat);
    • Alternate Names
      beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent for Serum & Plasma Samples Only
    4.   For Serum & Plasma Samples Only: Add 50 µL of Assay Diluent to each well.

    5. 200 µL Standard, Control, or Sample
    6.   Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well.
    10.   For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours.
      For Cell Culture Supernate & Urine Samples: Cover with a new plate sealer, and incubate at room temperature for 1 hour.
    11.   Aspirate and wash 3 times.

    12. 200 µL Substrate Solution
    13.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

    14. 50 µL Stop Solution
    15. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    43 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. The p38 signaling pathway mediates quiescence of glioma stem cells by regulating epidermal growth factor receptor trafficking
      Authors: A Soeda, J Lathia, BJ Williams, Q Wu, J Gallagher, A Androutsel, AJ Giles, C Yang, Z Zhuang, MR Gilbert, JN Rich, DM Park
      Oncotarget, 2017;8(20):33316-33328.
      Species: Human
      Sample Type: Cell Culture Supernates
    2. The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro
      Authors: M Bernardi, F Agostini, K Chieregato, E Amati, C Durante, M Rassu, M Ruggeri, S Sella, E Lombardi, M Mazzucato, G Astori
      J Transl Med, 2017;15(1):90.
      Species: Human
      Sample Type: Cell Lysates
    3. Inflammatory Differences in Plaque Erosion and Rupture in Patients With ST-Segment Elevation Myocardial Infarction
      Authors: S Chandran, J Watkins, A Abdul-Aziz, M Shafat, PA Calvert, KM Bowles, MD Flather, SA Rushworth, AD Ryding
      J Am Heart Assoc, 2017;6(5):.
      Species: Human
      Sample Type: Plasma
    4. Umbilical cord extracts improve diabetic abnormalities in bone marrow-derived mesenchymal stem cells and increase their therapeutic effects on diabetic nephropathy
      Authors: K Nagaishi, Y Mizue, T Chikenji, M Otani, M Nakano, Y Saijo, H Tsuchida, S Ishioka, A Nishikawa, T Saito, M Fujimiya
      Sci Rep, 2017;7(1):8484.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. Differential effects of amnion and chorion membrane extracts on osteoblast-like cells due to the different growth factor composition of the extracts
      Authors: YY Go, SE Kim, GJ Cho, SW Chae, JJ Song
      PLoS ONE, 2017;12(8):e0182716.
      Species: Human
      Sample Type: Tissue Homogenates
    6. Design, characterization and experimental validation of a compact, flexible pulsed power architecture for ex vivo platelet activation
      Authors: AL Garner, A Caiafa, Y Jiang, S Klopman, C Morton, AS Torres, AM Loveless, VB Neculaes
      PLoS ONE, 2017;12(7):e0181214.
      Species: Human
      Sample Type: Plasma
    7. Adipose-derived mesenchymal stem cells from patients with atherosclerotic renovascular disease have increased DNA damage and reduced angiogenesis that can be modified by hypoxia
      Stem Cell Res Ther, 2016;7(1):128.
      Species: Human
      Sample Type: Cell Culture Supernates
    8. Serum Interleukin-18, Fetuin-A, Soluble Intercellular Adhesion Molecule-1, and Endothelin-1 in Ankylosing Spondylitis, Psoriatic Arthritis, and SAPHO Syndrome
      Int J Mol Sci, 2016;17(8):.
      Species: Human
      Sample Type: Serum
    9. Transgenic Soybean Production of Bioactive Human Epidermal Growth Factor (EGF)
      Authors: Yonghua He
      PLoS ONE, 2016;11(6):e0157034.
      Species: Plant - Arabidopsis
      Sample Type: Protein
    10. N-Glycosylation of integrin ?5 acts as a switch for EGFR-mediated complex formation of integrin ?5?1 to ?6?4
      Sci Rep, 2016;6(0):33507.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. Endothelial cell-secreted EGF induces epithelial to mesenchymal transition and endows head and neck cancer cells with stem-like phenotype.
      Authors: Zhang Z, Dong Z, Lauxen I, Filho M, Nor J
      Cancer Res, 2014;74(10):2869-81.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Ex vivo expansion of bovine corneal endothelial cells in xeno-free medium supplemented with platelet releasate.
      Authors: Chou M, Burnouf T, Wang T
      PLoS ONE, 2014;9(6):e99145.
      Species: Human
      Sample Type: Cell Culture Supernates
    13. The evolution of cellular deficiency in GATA2 mutation.
      Authors: Dickinson R, Milne P, Jardine L, Zandi S, Swierczek S, McGovern N, Cookson S, Ferozepurwalla Z, Langridge A, Pagan S, Gennery A, Heiskanen-Kosma T, Hamalainen S, Seppanen M, Helbert M, Tholouli E, Gambineri E, Reykdal S, Gottfreethsson M, Thaventhiran J, Morris E, Hirschfield G, Richter A, Jolles S, Bacon C, Hambleton S, Haniffa M, Bryceson Y, Allen C, Prchal J, Dick J, Bigley V, Collin M
      Blood, 2014;123(6):863-74.
      Species: Human
      Sample Type: Serum
    14. Plasma biomarkers as predictors of outcome in patients with advanced hepatocellular carcinoma.
      Authors: Llovet JM, Pena CE, Lathia CD, Shan M, Meinhardt G, Bruix J, Pallota MG, Zarba JJ, Boyer M, Riordan S, Strickland A, Tebbutt N, Thomson B, Borbath I, De Greve J, Van Laethem J-, Van Steenbergen W, Van Vlierberghe H, Barrios C, Cosme de Oliveira A, Kotzev I, Takov D, Tchernev K, Burak K, Ma M, Metrakos P, Olweny C, Sherman M, Gamargo Garate C, Martinez-Castillo J, Beaugrand M, Bennouna J, Blanc J-, Bronowicki J-, Degos F, Dominguez S, Grange J-, Hillon P, Raoul J-, Seitz J-, Blum H, Buggisch P, Caspary W, Dollinger M, Gerken G, Goke B, Gregor M, Greten T, Haussinger D, Hilgard P, Scherübl J, Scheulen M, Schmid R, Spengler U, Wiest R, Zeuzem S, Arvanitakis C, Germanidis G, Katsos I, Figer A, Stemmer S, Amadori D, Bolondi L, Cognetti F, Craxi A, Farinati F, Gridelli C, Martoni A, Mazzaferro V, Porta C, Ricci S, Sangiovanni A, Santoro A, Trevisani F, Cisnero Garza LE, Gane E, O'Donnell A, Leon J, Lozano A, Jassem J, Rydzewska G, Szawlowski A, Tomczak P, Badulescu F, Miron L, Kubyshkin V, Bruix J, Forner A, Bustamante Schneider J, Diago M, Montero Alvarez JL, Pascual S, Ruíz del Arbol L, Sangro B, Solá R, Tabernero J, Muellhaupt B, Roth A, Jeffry Evans TR, Falk S, Meyer T, Reeves H, Ross P, Befeler A, Boyer T, Britten C, Byrne T, Garcia-Tsao G, Gold P, Goldenberg A, Heuman D, Kennedy P, Koch A, Llovet JM, Marrero J, Schilsky M, Schwartz J, Schwartz M
      Clin. Cancer Res., 2012;18(8):2290-300.
      Species: Human
      Sample Type: Plasma
    15. Activation of HER family signaling as a mechanism of acquired resistance to ALK inhibitors in EML4-ALK-positive non-small cell lung cancer.
      Authors: Tanizaki J, Okamoto I, Okabe T, Sakai K, Tanaka K, Hayashi H, Kaneda H, Takezawa K, Kuwata K, Yamaguchi H, Hatashita E, Nishio K, Nakagawa K
      Clin Cancer Res, 2012;18(22):6219-26.
      Species: Human
      Sample Type: Cell Culture Supernates
    16. Decreased plasma cytokines are associated with low platelet counts in aplastic anemia and immune thrombocytopenic purpura.
      J. Thromb. Haemost., 2012;10(8):1616-23.
      Species: Human
      Sample Type: Serum
    17. Expression and prognostic value of circulating angiogenic cytokines in pancreatic cancer.
      Authors: Rahbari NN, Schmidt T, Falk CS, Hinz U, Herber M, Bork U, Buchler MW, Weitz J, Koch M
      BMC Cancer, 2011;11(0):286.
      Species: Human
      Sample Type: Serum
    18. Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
      Authors: Rauch C, Feifel E, Amann EM, Spotl HP, Schennach H, Pfaller W, Gstraunthaler G
      ALTEX, 2011;28(4):305-16.
      Species: Human
      Sample Type: Cell Lysates
    19. Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans.
      Authors: Aronis KN, Diakopoulos KN, Fiorenza CG
      Diabetologia, 2011;54(9):2358-67.
      Species: Human
      Sample Type: Serum
    20. Vascular endothelial growth factor drives autocrine epithelial cell proliferation and survival in chronic rhinosinusitis with nasal polyposis.
      Authors: Lee HS, Myers A, Kim J
      Am. J. Respir. Crit. Care Med., 2009;180(11):1056-67.
      Species: Human
      Sample Type: Nasal Lavage
    21. Tumor-derived pancreatic stellate cells promote pancreatic cancer cell invasion through release of thrombospondin-2.
      Authors: Farrow B, Berger DH, Rowley D
      J. Surg. Res., 2009;156(1):155-60.
      Species: Human
      Sample Type: Cell Culture Supernates
    22. Molecular response to cetuximab and efficacy of preoperative cetuximab-based chemoradiation in rectal cancer.
      Authors: Debucquoy A, Haustermans K, Daemen A, Aydin S, Libbrecht L, Gevaert O, De Moor B, Tejpar S, McBride WH, Penninckx F, Scalliet P, Stroh C, Vlassak S, Sempoux C, Machiels JP
      J. Clin. Oncol., 2009;27(17):2751-7.
      Species: Human
      Sample Type: Plasma
    23. Phase II study and biomarker analysis of cetuximab combined with modified FOLFOX6 in advanced gastric cancer.
      Authors: Han SW, Oh DY, Im SA, Park SR, Lee KW, Song HS, Lee NS, Lee KH, Choi IS, Lee MH, Kim MA, Kim WH, Bang YJ, Kim TY
      Br. J. Cancer, 2009;100(2):298-304.
      Species: Human
      Sample Type: Serum
    24. Effect of protein kinase Cbeta inhibition on renal hemodynamic function and urinary biomarkers in humans with type 1 diabetes: a pilot study.
      Authors: Cherney DZ, Konvalinka A, Zinman B, Diamandis EP, Soosaipillai A, Reich H, Lorraine J, Lai V, Scholey JW, Miller JA
      Diabetes Care, 2009;32(1):91-3.
      Species: Human
      Sample Type: Urine
    25. Specific inhibition of tissue kallikrein 1 with a human monoclonal antibody reveals a potential role in airway diseases.
      Authors: Sexton DJ, Chen T, Martik D, Kuzmic P, Kuang G, Chen J, Nixon AE, Zuraw BL, Forteza RM, Abraham WM, Wood CR
      Biochem. J., 2009;422(2):383-92.
      Species: Human
      Sample Type: Cell Culture Supernates
    26. A urinary biomarker profile for children with HIV-associated renal diseases.
      Authors: Soler-Garcia AA, Rakhmanina NY, Mattison PC, Ray PE
      Kidney Int., 2009;76(2):207-14.
      Species: Human
      Sample Type: Urine
    27. Epidermal Growth Factor Receptor Blockade in Combination with Conventional Chemotherapy Inhibits Soft Tissue Sarcoma Cell Growth In vitro and In vivo.
      Authors: Ren W, Korchin B, Zhu QS, Wei C, Dicker A, Heymach J, Lazar A, Pollock RE, Lev D
      Clin. Cancer Res., 2008;14(9):2785-2795.
      Species: Human
      Sample Type: Cell Culture Supernates
    28. Effective inhibition of the epidermal growth factor/epidermal growth factor receptor binding by anti-epidermal growth factor antibodies is related to better survival in advanced non-small-cell lung cancer patients treated with the epidermal growth factor cancer vaccine.
      Authors: Garcia B, Neninger E, de la Torre A, Leonard I, Martinez R, Viada C, Gonzalez G, Mazorra Z, Lage A, Crombet T
      Clin. Cancer Res., 2008;14(3):840-6.
      Species: Human
      Sample Type: Serum
    29. The levels of steroid hormones and cytokines in individual follicles are not associated with the fertilization outcome after intracytoplasmic sperm injection.
      Authors: Asimakopoulos B, Abu-Hassan D, Metzen E, Al-Hasani S, Diedrich K, Nikolettos N
      Fertil. Steril., 2007;90(1):60-4.
      Species: Human
      Sample Type: Follicular Fluid
    30. Alteration of the serum levels of the epidermal growth factor receptor and its ligands in patients with non-small cell lung cancer and head and neck carcinoma.
      Authors: Lemos-Gonzalez Y, Rodriguez-Berrocal FJ, Cordero OJ, Gomez C, Paez de la Cadena M
      Br. J. Cancer, 2007;96(10):1569-78.
      Species: Human
      Sample Type: Serum
    31. Human bone marrow activates the Akt pathway in metastatic prostate cells through transactivation of the alpha-platelet-derived growth factor receptor.
      Authors: Dolloff NG, Russell MR, Loizos N, Fatatis A
      Cancer Res., 2007;67(2):555-62.
      Species: Human
      Sample Type: Cell Culture Supernates
    32. Cytokine profiling in human colostrum and milk by protein array.
      Authors: Kverka M, Burianova J, Lodinova-Zadnikova R, Kocourkova I, Cinova J, Tuckova L, Tlaskalova-Hogenova H
      Clin. Chem., 2007;53(5):955-62.
      Species: Human
      Sample Type: Milk
    33. Selective cyclooxygenase-2 inhibitor downregulates the paracrine epithelial-mesenchymal interactions of growth in scirrhous gastric carcinoma.
      Authors: Yashiro M, Nakazawa K, Tendo M, Kosaka K, Shinto O, Hirakawa K
      Int. J. Cancer, 2007;120(3):686-93.
      Species: Human
      Sample Type: Cell Culture Supernates
    34. Salivary leptin induces increased expression of growth factors in oral keratinocytes.
      Authors: Groschl M, Topf HG, Kratzsch J, Dotsch J, Rascher W, Rauh M
      J. Mol. Endocrinol., 2005;34(2):353-66.
      Species: Human
      Sample Type: Cell Culture Supernates
    35. Salivary epidermal growth factor in oral cavity cancer.
      Authors: Balicki R, Grabowska SZ, Citko A
      Oral Oncol., 2005;41(1):48-55.
      Species: Human
      Sample Type: Saliva
    36. A potential pro-angiogenic cell therapy with human placenta-derived mesenchymal cells.
      Authors: Nishishita T, Ouchi K, Zhang X, Inoue M, Inazawa T, Yoshiura K, Kuwabara K, Nakaoka T, Watanabe N, Igura K, Takahashi TA, Yamashita N
      Biochem. Biophys. Res. Commun., 2004;325(1):24-31.
      Species: Human
      Sample Type: Cell Lysates
    37. Factors associated with the outcomes of children with unilateral ureteropelvic junction obstruction.
      Authors: Chiou YY, Chiu NT, Wang ST, Cheng HL, Tang MJ
      J. Urol., 2004;171(1):397-402; disc.
      Species: Human
      Sample Type: Urine
    38. Alterations in antioxidant power and levels of epidermal growth factor and nitric oxide in saliva of patients with inflammatory bowel diseases.
      Authors: Jahanshahi G, Motavasel V, Rezaie A, Hashtroudi AA, Daryani NE, Abdollahi M
      Dig. Dis. Sci., 2004;49(11):1752-7.
      Species: Human
      Sample Type: Plasma
    39. AF5, a CNS cell line immortalized with an N-terminal fragment of SV40 large T: growth, differentiation, genetic stability, and gene expression.
      Authors: Truckenmiller ME, Vawter MP, Zhang P, Conejero-Goldberg C, Dillon-Carter O, Morales N, Cheadle C, Becker KG, Freed WJ
      Exp. Neurol., 2002;175(0):318.
      Species: Rat
      Sample Type: Cell Culture Supernates
    40. The significance of cytokines, chemical composition, and murine embryo development in hydrosalpinx fluid for predicting the IVF outcome in women with hydrosalpinx.
      Authors: Chen CD, Yang JH, Lin KC, Chao KH, Ho HN, Yang YS
      Hum. Reprod., 2002;17(1):128-33.
      Species: Human
      Sample Type: Hydrosalpinx Fluid
    41. Saliva analysis in the clinical setting: revisiting an underused diagnostic tool.
      Authors: Nagler RM, Hershkovich O, Lischinsky S, Diamond E, Reznick AZ
      J. Investig. Med., 2002;50(3):214-25.
      Species: Human
      Sample Type: Saliva
    42. Epithelial cells in nasal fluids from patients with allergic rhinitis: how do they relate to epidermal growth factor, eosinophils and eosinophil cationic protein?
      Authors: Benson M, Uddman R, Cardell LO
      Acta Otolaryngol., 2002;122(2):202-5.
      Species: Human
      Sample Type: Nasal Lavage
    43. Diminished epidermal growth factor levels in infants with necrotizing enterocolitis.
      Authors: Shin CE, Falcone RA, Stuart L, Erwin CR, Warner BW
      J. Pediatr. Surg., 2000;35(2):173-6; discus.
      Species: Human
      Sample Type: Serum
    Expand to show all 43 Citations
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