Human EGFR F(ab')2 (Research Grade Cetuximab Biosimilar) Antibody

R&D Systems | Catalog # MAB9577-FAB2

Recombinant Monoclonal Antibody For Research Use Only
R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu1F2
View all EGFR Primary Antibodies »

Product Specifications

Immunogen

Human EGFR/ErbB1

Specificity

Detects human EGFR based on Cetuximab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Cetuximab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Scientific Data Images for Human EGFR F(ab')2 (Research Grade Cetuximab Biosimilar) Antibody

Detection of EGFR in A431 cells by Flow Cytometry.

A431 cells were stained with Human Anti-Human EGFR F(ab')2 (Research Grade Cetuximab Biosimilar) Monoclonal Antibody (Catalog # MAB9577-fab2, filled histogram) or isotype control antibody (Catalog # 1-001-A, open histogram), followed by Goat Anti-Human IgG Allophycocyanin (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Applications for Human EGFR F(ab')2 (Research Grade Cetuximab Biosimilar) Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: A431 human epithelial carcinoma cell line

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EGFR

Cetuximab is a biosimilar directed against the extracellular domain of EGFR. This product constitutes the immunogen used for the anti-Idiotype catalog number MAB9626. The biotinylated antibody (catalog number FAB9577B) can be used in flow cytometry or various assay format to measure EGFR expression and can be detected by conjugated streptavidin.

Long Name

Epidermal Growth Factor Receptor

Alternate Names

EGF R, ErbB, ErbB1, HER-1

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Gene Symbol

EGFR

Additional EGFR Products

Product Documents for Human EGFR F(ab')2 (Research Grade Cetuximab Biosimilar) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human EGFR F(ab')2 (Research Grade Cetuximab Biosimilar) Antibody

For research use only

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Protocols

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FAQs

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