Human Endoglin/CD105 APC-conjugated Antibody

(3 citations)   
  • Species Reactivity
  • Specificity
    Detects human Endoglin/CD105.
  • Source
    Monoclonal Mouse IgG1 Clone # 166707
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Endoglin/CD105
    Accession # Q5T9B9
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Endoglin/CD105 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Endoglin/CD105 APC-conjugated Mono­clonal Antibody (Catalog # FAB10971A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Endoglin/CD105

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1‑3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% aa identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2‑5). Human Endoglin cDNA encodes 658 amino acids (aa) including a 25 aa signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain and a 47 aa cytoplasmic domain (1‑3). An isoform with a 14 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The human Endoglin ECD shares 65-72% aa identity with mouse, rat, bovine, porcine and canine Endoglin. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2), but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2‑4, 12‑14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFLT1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).

  • References:
    1. Gougos, A. and Letarte, M. (1990) J. Biol. Chem. 265:8361.
    2. ten Dijke, P. et al. (2008) Angiogenesis 11:79.
    3. Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
    4. Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
    5. Moody, J.L. et al. (2007) Stem Cells 25:2809.
    6. Velasco, S. et al. (2008) J. Cell Sci. 121:913.
    7. Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
    8. Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
    9. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
    10. Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
    11. Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
    12. Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
    13. Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
    14. Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.    
    15. Venkatesha, S. et al. (2006) Nat. Med. 12:642.
  • Entrez Gene IDs:
    2022 (Human); 13805 (Mouse); 497010 (Rat)
  • Alternate Names:
    CD105 antigen; CD105; endoglin; ENDOsler-Rendu-Weber syndrome 1; ENG; HHT1FLJ41744; ORW; ORW1
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 3 of 3
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Sample Type
  1. Microvesicles from Mesenchymal Stromal Cells Are Involved in HPC-Microenvironment Crosstalk in Myelodysplastic Patients.
    Authors: Muntion S, Ramos T, Diez-Campelo M, Roson B, Sanchez-Abarca L, Misiewicz-Krzeminska I, Preciado S, Sarasquete M, De Las Rivas J, Gonzalez M, Sanchez-Guijo F, Del Canizo M
    PLoS ONE, 2016;11(2):e0146722.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. MSC surface markers (CD44, CD73, and CD90) can identify human MSC-derived extracellular vesicles by conventional flow cytometry.
    Authors: L Ramos T, Sanchez-Abarca L, Muntion S, Preciado S, Puig N, Lopez-Ruano G, Hernandez-Hernandez A, Redondo A, Ortega R, Rodriguez C, Sanchez-Guijo F, Del Canizo C
    Cell Commun Signal, 2016;14(1):2.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Efficient differentiation of human pluripotent stem cells into mesenchymal stem cells by modulating intracellular signaling pathways in a feeder/serum-free system.
    Authors: Tran NT, Trinh QM, Lee GM
    Stem Cells Dev., 2012;21(7):1165-75.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 5  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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