Human Endoglin/CD105 PE-conjugated Antibody

(17 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Endoglin/CD105.
  • Source
    Monoclonal Mouse IgG1 Clone # 166707
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Endoglin/CD105
    Glu26-Gly586
    Accession # Q5T9B9
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Endoglin/CD105 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Endoglin/CD105 PE-conjugated Mono­clonal Antibody (Catalog # FAB10971P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Endoglin/CD105

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1‑3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% aa identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Human Endoglin cDNA encodes 658 amino acids (aa) including a 25 aa signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain and a 47 aa cytoplasmic domain (1-3). An isoform with a 14 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The human Endoglin ECD shares 65-72% aa identity with mouse, rat, bovine, porcine and canine Endoglin. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2), but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2‑4, 12‑14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFLT1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).

  • References:
    1. Gougos, A. and Letarte, M. (1990) J. Biol. Chem. 265:8361.
    2. ten Dijke, P. et al. (2008) Angiogenesis 11:79.
    3. Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
    4. Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
    5. Moody, J.L. et al. (2007) Stem Cells 25:2809.
    6. Velasco, S. et al. (2008) J. Cell Sci. 121:913.
    7. Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
    8. Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
    9. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
    10. Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
    11. Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
    12. Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
    13. Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
    14. Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.    
    15. Venkatesha, S. et al. (2006) Nat. Med. 12:642.
  • Entrez Gene IDs:
    2022 (Human); 13805 (Mouse); 497010 (Rat)
  • Alternate Names:
    CD105 antigen; CD105; Endoglin; ENDOsler-Rendu-Weber syndrome 1; ENG; HHT1FLJ41744; ORW; ORW1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Therapeutic microparticles functionalized with biomimetic cardiac stem cell membranes and secretome
    Authors: J Tang, D Shen, TG Caranasos, Z Wang, AC Vandergrif, TA Allen, MT Hensley, PU Dinh, J Cores, TS Li, J Zhang, Q Kan, K Cheng
    Nat Commun, 2017;8(0):13724.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Study of the microRNA expression profile of foreskin derived mesenchymal stromal cells following inflammation priming
    Authors: H Fayyad-Kaz, M Fayyad-Kaz, B Badran, D Bron, L Lagneaux, M Najar
    J Transl Med, 2017;15(1):10.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer
    Authors: PL Mok, SN Leow, AE Koh, HH Mohd Nizam, SL Ding, C Luu, R Ruhaslizan, HS Wong, WH Halim, MH Ng, RB Idrus, SR Chowdhury, CM Bastion, SK Subbiah, A Higuchi, AA Alarfaj, KY Then
    Int J Mol Sci, 2017;18(2):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Derivation of therapeutic lung spheroid cells from minimally invasive transbronchial pulmonary biopsies
    Authors: PC Dinh, J Cores, MT Hensley, AC Vandergrif, J Tang, TA Allen, TG Caranasos, KB Adler, LJ Lobo, K Cheng
    Respir. Res., 2017;18(1):132.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum, Human Serum, and Defined Xeno-Free/Serum-Free Conditions
    Authors: M Patrikoski, MHC Lee, L Mäkinen, XM Ang, B Mannerströ, M Raghunath, S Miettinen
    Stem Cells Int, 2017;2017(0):6909163.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. The differentiation potential of gingival mesenchymal stem cells induced by apical tooth germ cell?conditioned medium
    Mol Med Rep, 2016;14(4):3565-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. The effect of pro-inflammatory cytokines on immunophenotype, differentiation capacity and immunomodulatory functions of human mesenchymal stem cells
    Authors: Arash Pourgholam
    Cytokine, 2016;85(0):51-60.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Effect of Secreted Molecules of Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells on Acute Hepatic Failure Model
    Stem Cells Dev, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Amniotic Fluid Derived Stem Cells with a Renal Progenitor Phenotype Inhibit Interstitial Fibrosis in Renal Ischemia and Reperfusion Injury in Rats.
    Authors: Monteiro Carvalho Mori da M, Zia S, Oliveira Arcolino F, Carlon M, Beckmann D, Pippi N, Luhers Graca D, Levtchenko E, Deprest J, Toelen J
    PLoS ONE, 2015;10(8):e0136145.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R.
    Authors: Mirkina I, Hadzijusufovic E, Krepler C, Mikula M, Mechtcheriakova D, Strommer S, Stella A, Jensen-Jarolim E, Holler C, Wacheck V, Pehamberger H, Valent P
    PLoS ONE, 2014;9(1):e84417.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. Adipose stem cells used to reconstruct 13 cases with cranio-maxillofacial hard-tissue defects.
    Authors: Sandor G, Numminen J, Wolff J, Thesleff T, Miettinen A, Tuovinen V, Mannerstrom B, Patrikoski M, Seppanen R, Miettinen S, Rautiainen M, Ohman J
    Stem Cells Transl Med, 2014;3(4):530-40.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. Characterisation of syncytiotrophoblast vesicles in normal pregnancy and pre-eclampsia: expression of Flt-1 and endoglin.
    Authors: Tannetta D, Dragovic R, Gardiner C, Redman C, Sargent I
    PLoS ONE, 2013;8(2):e56754.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  13. Human dermis harbors distinct mesenchymal stromal cell subsets.
    Authors: Vaculik C, Schuster C, Bauer W, Iram N, Pfisterer K, Kramer G, Reinisch A, Strunk D, Elbe-Burger A
    J. Invest. Dermatol., 2012;132(3):563-74.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  14. TGFbeta inhibition enhances the generation of hematopoietic progenitors from human ES cell-derived hemogenic endothelial cells using a stepwise strategy.
    Authors: Wang C, Tang X, Sun X, Miao Z, Lv Y, Yang Y, Zhang H, Zhang P, Liu Y, DU L, Gao Y, Yin M, Ding M, Deng H
    Cell Res., 2012;22(1):194-207.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  15. Transplantation of CTLA4Ig gene-transduced adipose tissue-derived mesenchymal stem cells reduces inflammatory immune response and improves Th1/Th2 balance in experimental autoimmune thyroiditis.
    Authors: Choi EW, Shin IS, Lee HW, Park SY, Park JH, Nam MH, Kim JS, Woo SK, Yoon EJ, Kang SK, Ra JC, Youn HY, Hong SH
    J Gene Med, 2011;13(1):3-16.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  16. Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.
    Authors: Giuliani M, Fleury M, Vernochet A, Ketroussi F, Clay D, Azzarone B, Lataillade JJ, Durrbach A
    2011;6(5):e19988.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  17. Expression of an exogenous human Oct-4 promoter identifies tumor-initiating cells in osteosarcoma.
    Authors: Levings PP, McGarry SV, Currie TP, Nickerson DM, McClellan S, Ghivizzani SC, Steindler DA, Gibbs CP
    Cancer Res., 2009;69(14):5648-55.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 17 Citations
Isotype Controls
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Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 38  
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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