Human Endoglin/CD105 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB10971P-100
FAB10971P-025
Human Endoglin/CD105 PE-conjugated Antibody in Data
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Product Details
Citations (26)
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Human Endoglin/CD105 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Endoglin/CD105.
Source
Monoclonal Mouse IgG1 Clone # 166707
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Endoglin/CD105
Glu26-Gly586
Accession # Q5T9B9
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Endoglin/CD105 antibody in U937 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Endoglin/CD105 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Endoglin/CD105 PE-conjugated Mono-clonal Antibody (Catalog # FAB10971P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Endoglin/CD105

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1‑3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% aa identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Human Endoglin cDNA encodes 658 amino acids (aa) including a 25 aa signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain and a 47 aa cytoplasmic domain (1-3). An isoform with a 14 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The human Endoglin ECD shares 65-72% aa identity with mouse, rat, bovine, porcine and canine Endoglin. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2), but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2‑4, 12‑14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFLT1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).

References
  1. Gougos, A. and Letarte, M. (1990) J. Biol. Chem. 265:8361.
  2. ten Dijke, P. et al. (2008) Angiogenesis 11:79.
  3. Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
  4. Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
  5. Moody, J.L. et al. (2007) Stem Cells 25:2809.
  6. Velasco, S. et al. (2008) J. Cell Sci. 121:913.
  7. Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
  8. Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
  9. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
  10. Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
  11. Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
  12. Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
  13. Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
  14. Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.    
  15. Venkatesha, S. et al. (2006) Nat. Med. 12:642.
Entrez Gene IDs
2022 (Human); 13805 (Mouse); 497010 (Rat)
Alternate Names
CD105 antigen; CD105; Endoglin; ENDOsler-Rendu-Weber syndrome 1; ENG; HHT1FLJ41744; ORW; ORW1

Product Datasheets

Citations for Human Endoglin/CD105 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. Myocardin-Related Transcription Factor A (MRTF-A) Regulates the Balance between Adipogenesis and Osteogenesis of Human Adipose Stem Cells
    Authors: L Hyväri, S Vanhatupa, HT Halonen, M Kääriäinen, S Miettinen
    Stem Cells Int, 2020;2020(0):8853541.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Cell adhesion and culture medium dependent changes in the high frequency mechanical vibration induced proliferation, osteogenesis, and intracellular organization of human adipose stem cells
    Authors: HT Halonen, TO Ihalainen, L Hyväri, S Miettinen, JAK Hyttinen
    J Mech Behav Biomed Mater, 2019;101(0):103419.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Human menstrual blood-derived stromal/stem cells modulate functional features of natural killer cells
    Authors: MR Shokri, M Bozorgmehr, A Ghanavatin, R Falak, M Aleahmad, S Kazemnejad, F Shokri, AH Zarnani
    Sci Rep, 2019;9(1):10007.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. LncRNA-TWIST1 Promoted Osteogenic Differentiation Both in PPDLSCs and in HPDLSCs by Inhibiting TWIST1 Expression
    Authors: Y Xu, W Qin, D Guo, J Liu, M Zhang, Z Jin
    Biomed Res Int, 2019;2019(0):8735952.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Functional Outcome of Human Adipose Stem Cell Injections in Rat Anal Sphincter Acute Injury Model
    Authors: K Kuismanen, M Juntunen, N Narra Giri, H Tuominen, H Huhtala, K Nieminen, J Hyttinen, S Miettinen
    Stem Cells Transl Med, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Focal Adhesion Kinase and ROCK Signaling Are Switch-Like Regulators of Human Adipose Stem Cell Differentiation towards Osteogenic and Adipogenic Lineages
    Authors: L Hyväri, M Ojansivu, M Juntunen, K Kartasalo, S Miettinen, S Vanhatupa
    Stem Cells Int, 2018;2018(0):2190657.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. The effect of S53P4-based borosilicate glasses and glass dissolution products on the osteogenic commitment of human adipose stem cells
    Authors: M Ojansivu, A Mishra, S Vanhatupa, M Juntunen, A Larionova, J Massera, S Miettinen
    PLoS ONE, 2018;13(8):e0202740.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Effect of intensive multifactorial treatment on vascular progenitor cells in hypertensive patients
    Authors: C Maroun-Eid, A Ortega-Her, J Modrego, M Abad-Cardi, JA García-Don, L Reinares, N Martell-Cl, D Gómez-Garr
    PLoS ONE, 2018;13(1):e0190494.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Study of the microRNA expression profile of foreskin derived mesenchymal stromal cells following inflammation priming
    Authors: H Fayyad-Kaz, M Fayyad-Kaz, B Badran, D Bron, L Lagneaux, M Najar
    J Transl Med, 2017;15(1):10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Therapeutic microparticles functionalized with biomimetic cardiac stem cell membranes and secretome
    Authors: J Tang, D Shen, TG Caranasos, Z Wang, AC Vandergrif, TA Allen, MT Hensley, PU Dinh, J Cores, TS Li, J Zhang, Q Kan, K Cheng
    Nat Commun, 2017;8(0):13724.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer
    Authors: PL Mok, SN Leow, AE Koh, HH Mohd Nizam, SL Ding, C Luu, R Ruhaslizan, HS Wong, WH Halim, MH Ng, RB Idrus, SR Chowdhury, CM Bastion, SK Subbiah, A Higuchi, AA Alarfaj, KY Then
    Int J Mol Sci, 2017;18(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Derivation of therapeutic lung spheroid cells from minimally invasive transbronchial pulmonary biopsies
    Authors: PC Dinh, J Cores, MT Hensley, AC Vandergrif, J Tang, TA Allen, TG Caranasos, KB Adler, LJ Lobo, K Cheng
    Respir. Res., 2017;18(1):132.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum, Human Serum, and Defined Xeno-Free/Serum-Free Conditions
    Authors: M Patrikoski, MHC Lee, L Mäkinen, XM Ang, B Mannerströ, M Raghunath, S Miettinen
    Stem Cells Int, 2017;2017(0):6909163.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Cranioplasty with Adipose-Derived Stem Cells, Beta-Tricalcium Phosphate Granules and Supporting Mesh: Six-Year Clinical Follow-Up Results
    Authors: T Thesleff, K Lehtimäki, T Niskakanga, S Huovinen, B Mannerströ, S Miettinen, R Seppänen-K, J Öhman
    Stem Cells Transl Med, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Effect of Secreted Molecules of Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells on Acute Hepatic Failure Model
    Stem Cells Dev, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. The effect of pro-inflammatory cytokines on immunophenotype, differentiation capacity and immunomodulatory functions of human mesenchymal stem cells
    Authors: Arash Pourgholam
    Cytokine, 2016;85(0):51-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. The differentiation potential of gingival mesenchymal stem cells induced by apical tooth germ cell?conditioned medium
    Mol Med Rep, 2016;14(4):3565-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Amniotic Fluid Derived Stem Cells with a Renal Progenitor Phenotype Inhibit Interstitial Fibrosis in Renal Ischemia and Reperfusion Injury in Rats.
    Authors: Monteiro Carvalho Mori da M, Zia S, Oliveira Arcolino F, Carlon M, Beckmann D, Pippi N, Luhers Graca D, Levtchenko E, Deprest J, Toelen J
    PLoS ONE, 2015;10(8):e0136145.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R.
    Authors: Mirkina I, Hadzijusufovic E, Krepler C, Mikula M, Mechtcheriakova D, Strommer S, Stella A, Jensen-Jarolim E, Holler C, Wacheck V, Pehamberger H, Valent P
    PLoS ONE, 2014;9(1):e84417.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Adipose stem cells used to reconstruct 13 cases with cranio-maxillofacial hard-tissue defects.
    Authors: Sandor G, Numminen J, Wolff J, Thesleff T, Miettinen A, Tuovinen V, Mannerstrom B, Patrikoski M, Seppanen R, Miettinen S, Rautiainen M, Ohman J
    Stem Cells Transl Med, 2014;3(4):530-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Characterisation of syncytiotrophoblast vesicles in normal pregnancy and pre-eclampsia: expression of Flt-1 and endoglin.
    Authors: Tannetta D, Dragovic R, Gardiner C, Redman C, Sargent I
    PLoS ONE, 2013;8(2):e56754.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Human dermis harbors distinct mesenchymal stromal cell subsets.
    Authors: Vaculik C, Schuster C, Bauer W, Iram N, Pfisterer K, Kramer G, Reinisch A, Strunk D, Elbe-Burger A
    J. Invest. Dermatol., 2012;132(3):563-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. TGFbeta inhibition enhances the generation of hematopoietic progenitors from human ES cell-derived hemogenic endothelial cells using a stepwise strategy.
    Authors: Wang C, Tang X, Sun X, Miao Z, Lv Y, Yang Y, Zhang H, Zhang P, Liu Y, DU L, Gao Y, Yin M, Ding M, Deng H
    Cell Res., 2012;22(1):194-207.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.
    Authors: Giuliani M, Fleury M, Vernochet A, Ketroussi F, Clay D, Azzarone B, Lataillade JJ, Durrbach A
    2011;6(5):e19988.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Transplantation of CTLA4Ig gene-transduced adipose tissue-derived mesenchymal stem cells reduces inflammatory immune response and improves Th1/Th2 balance in experimental autoimmune thyroiditis.
    Authors: Choi EW, Shin IS, Lee HW, Park SY, Park JH, Nam MH, Kim JS, Woo SK, Yoon EJ, Kang SK, Ra JC, Youn HY, Hong SH
    J Gene Med, 2011;13(1):3-16.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Expression of an exogenous human Oct-4 promoter identifies tumor-initiating cells in osteosarcoma.
    Authors: Levings PP, McGarry SV, Currie TP, Nickerson DM, McClellan S, Ghivizzani SC, Steindler DA, Gibbs CP
    Cancer Res., 2009;69(14):5648-55.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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