Human EphB6 Antibody

Catalog # Availability Size / Price Qty
AF3384
AF3384-SP
Detection  of EphB6 in MOLT‑4 Human Cell Line by Flow Cytometry.
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Human EphB6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human EphB6 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse (rm) EphB6 is observed and less than 1% cross-reactivity with recombinant human (rh) EphB4 and rhEphA2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human EphB6
Leu17 - Ser579
Accession # O15197
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human EphB6 Fc Chimera (Catalog # 3384-B6)
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of EphB6 in MOLT‑4 Human Cell Line by Flow Cytometry. View Larger

Detection of EphB6 in MOLT‑4 Human Cell Line by Flow Cytometry. MOLT‑4 human acute lymphoblastic leukemia cell line was stained with Sheep Anti-Human EphB6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3384, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphB6

EphB6, also known as Hep and Mep, is a 110 kDa member of the Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by ligand preference and have a common structural organization (1‑4). The human EphB6 cDNA encodes a 1006 amino acid (aa) precursor that includes a 16 aa signal sequence, a 563 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 406 aa cytoplasmic domain. The ECD contains serine- and cysteine-rich regions and two fibronectin type-III domains. The cytoplasmic domain contains one non-catalytic protein kinase-like, one proline-rich, one SAM, and one PDZ-binding domain (5, 6). Within the ECD, human EphB6 shares 91% aa sequence identity with mouse and rat EphB6. It shares 38‑45% aa sequence identity with human EphB1, 2, 3, 4, and 6. Human EphB5 has not been characterized. Two secreted splice variants have been described in mouse but not in human (6). EphB6 is primarily expressed in brain, pancreas, thymus, and peripheral T cells (5, 7, 8). EphB6 forms stable heterodimers with EphB1 and participates in signal transduction by association with other enzymatically active molecules (9‑11). Ephrin‑B2 is the dominant ligand for EphB6, although Ephrin‑B1 and Ephrin‑B3 can also trigger responses (12‑14). High concentrations of Ephrin‑B2 inhibit cell adhesion and migration as well as tyrosine phosphorylation of EphB6. Conversely, low concentrations of Ephrin‑B2 promote adhesion and migration and do not lead to EphB6 phosphorylation (15). The level of EphB6 expression is inversely correlated with tumor aggressiveness in a variety of malignancies (1). EphB6 also functions as a T cell co-stimulatory molecule (8, 11, 13). EphB6 clusters with the T cell receptor and participates in the subsequent attenuation of the T cell response (8, 10, 11, 13).

References
  1. Surawska, H. et al. (2004) Cytokine Growth Factor Rev. 15:419.
  2. Poliakov, A. et al. (2004) Dev. Cell 7:465.
  3. Wu, J. and H. Luo (2005) Curr. Opin. Hematol. 12:292.
  4. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
  5. Matsuoka, H. et al. (1997) Biochem. Biophys. Res. Commun. 235:487.
  6. Gurniak, C.B. and L.J. Berg (1996) Oncogene 13:777.
  7. Hafner, C. et al. (2004) Clin. Chem. 50:490.
  8. Luo, H. et al. (2002) J. Clin. Invest. 110:1141.
  9. Freywald, A. et al. (2002) J. Biol. Chem. 277:3823.
  10. Freywald, A. et al. (2003) J. Biol. Chem. 278:10150.
  11. Luo, H. et al. (2001) J. Immunol. 167:1362.
  12. Munthe, E. et al. (2000) FEBS Lett. 466:169.
  13. Luo, H. et al. (2004) J. Clin. Invest. 114:1762.
  14. Shimoyama, M. et al. (2002) Biochem. Biophys. Res. Commun. 298:87.
  15. Matsuoka, H. et al. (2005) J. Biol. Chem. 280:29355.
Long Name
Eph Receptor B6
Entrez Gene IDs
2051 (Human); 13848 (Mouse)
Alternate Names
EC 2.7.10.1; EPH receptor B6; EphB6; ephrin type-B receptor 6; Hep; HEPTyrosine-protein kinase-defective receptor EPH-6; Mep; MGC129910; MGC129911

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