Human FABP2/I-FABP Quantikine ELISA Kit Summary
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine
|Intra-Assay Precision||Inter-Assay Precision|
The recovery of human FABP2 spiked to levels throughout the range of the assay in various matrices was evaluated.
|Sample Type||Average % Recovery||Range %|
|Cell Culture Media (n=4)||100||96-104|
|Cell Lysis Buffer (n=2)||83||81-85|
|EDTA Plasma (n=4)||97||85-102|
|Heparin Plasma (n=4)||95||96-104|
Preparation and Storage
Fatty acid binding proteins are small cytoplasmic lipid binding proteins that are expressed in a tissue specific manner. FABPs bind free fatty acids, cholesterol, and retinoids, and are involved in intracellular lipid transport. Circulating FABP levels are used as indicators of tissue damage. Some FABP polymorphisms have been associated with disorders of lipid metabolism and the development of atherosclerosis.
FABP2, also known as intestinal fatty acid binding protein (I-FABP or FABPI) and gut FABP (gFABP), is a member of the cytosolic fatty acid binding protein family. FABP2 mediates the absorption and intracellular transport of dietary long-chain fatty acids. Genetic variations of FABP2 are implicated in obesity and Type II diabetes. Human FABP2 shares 78%, 82%, and 86% amino acid sequence identity with mouse, rat, and canine FABP2, respectively.
Citations for Human FABP2/I-FABP Quantikine ELISA Kit
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Hypoallergenic and anti-inflammatory feeds in children with complicated severe acute malnutrition: an open randomised controlled 3-arm intervention trial in Malawi
Authors: RH Bartels, E Chimwezi, V Watson, L Pei, I Potani, B Allubha, K Chidzalo, D Wang, Q Dube, M Mallewa, A Allen, RHJ Bandsma, WP Voskuijl, SJ Allen
Sci Rep, 2019;9(1):2304.
Sample Types: Plasma
Relation between postoperative ileus and anastomotic leakage after colorectal resection: a post hoc analysis of a prospective randomized controlled trial
Authors: E G Peters
Colorectal Dis, 2016;0(0):.
Sample Types: Plasma
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Average Rating: 4.7 (Based on 3 Reviews)
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The Quantikine ELISA kit worked as expected with low % CV's, and reproducible results.