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Human FABP2/I-FABP Quantikine ELISA Kit

R&D Systems | Catalog # DFBP20

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL)

Sensitivity

6.21 pg/mL

Assay Range

15.6-1000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
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Product Summary for Human FABP2/I-FABP Quantikine ELISA Kit

The Quantikine Human FABP2/I-FABP Immunoassay is a 4.5 hour solid phase ELISA designed to measure FABP2 levels in cell culture supernates, cell lysates, serum, plasma, and urine. It contains E. coli-expressed recombinant human FABP2 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human FABP2 showed linear curves that were parallel to the standard curves obtained using the Quantikine Human FABP2 Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human FABP2.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human FABP2

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Sample Values

Serum/Plasma/Urine - Samples from apparently healthy volunteers were evaluated for the presence of human FABP2 in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean (pg/mL)Range (pg/mL)Standard Deviation (pg/mL)
Serum (n=36)1015389-2129455
EDTA plasma952377-2049444
Heparin plasma (n=36)797230-1800381


Sample TypeMean of Detectable (pg/mL)% DetectableRange (pg/mL)
Urine (n=10)18490ND-632
ND=Non-detectable

Cell Culture Supernates - Human peripheral blood leukocytes (PBL) were cultured in RPMI 1640 and supplemented with 10% fetal bovine serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were then cultured unstimulated or stimulated with 10 μg/mL PHA for 1 or 6 days. Aliquots of the cell culture supernates were removed and assayed for levels of human FABP2. No detectable levels were observed.

Cell Lysates - LoVo human colorectal adenocarcinoma cells were cultured in Kaighn's supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin sulfate, and 1.5 g/L sodium bicarbonate and grown until confluent. An aliquot of the cell lysate was removed, assayed for human FABP2, and measured 82.3 pg/mg.


Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 77.5 207 425 80.5 216 446
Standard Deviation 3.14 7.24 12.4 8.94 17.4 26.6
CV% 4.1 3.5 2.9 11.1 8.1 6.0

Recovery for Human FABP2/I-FABP Quantikine ELISA Kit

The recovery of human FABP2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 96-104
Cell Lysis Buffer (n=2) 83 81-85
EDTA Plasma (n=4) 97 85-102
Heparin Plasma (n=4) 95 96-104
Serum (n=4) 101 97-106
Urine (n=4) 90 85-95

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human FABP2 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human FABP2/I-FABP ELISA Linearity

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FABP2/I-FABP

Fatty acid binding proteins are small cytoplasmic lipid binding proteins that are expressed in a tissue specific manner. FABPs bind free fatty acids, cholesterol, and retinoids, and are involved in intracellular lipid transport. Circulating FABP levels are used as indicators of tissue damage. Some FABP polymorphisms have been associated with disorders of lipid metabolism and the development of atherosclerosis.

FABP2, also known as intestinal fatty acid binding protein (I-FABP or FABPI) and gut FABP (gFABP), is a member of the cytosolic fatty acid binding protein family. FABP2 mediates the absorption and intracellular transport of dietary long-chain fatty acids. Genetic variations of FABP2 are implicated in obesity and Type II diabetes. Human FABP2 shares 78%, 82%, and 86% amino acid sequence identity with mouse, rat, and canine FABP2, respectively.

Long Name

Fatty Acid-Binding Protein 2/Intestinal FABP

Alternate Names

I-FABP, IFABP, Intestinal FABP

Entrez Gene IDs

2169 (Human); 25598 (Rat)

Gene Symbol

FABP2

Additional FABP2/I-FABP Products

Product Documents for Human FABP2/I-FABP Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human FABP2/I-FABP Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Related Research Areas

Early Endodermal Lineage Markers
Obesity

Citations for Human FABP2/I-FABP Quantikine ELISA Kit

Customer Reviews for Human FABP2/I-FABP Quantikine ELISA Kit (3)

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  • Human FABP2/I-FABP Quantikine ELISA Kit
    Name: Adriana Trajtman
    Sample Tested: blood and respiratory
    Verified Customer | Posted 10/20/2017
  • Human FABP2/I-FABP Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 09/07/2017
    The Quantikine ELISA kit worked as expected with low % CV's, and reproducible results.
    Human FABP2/I-FABP Quantikine ELISA Kit DFBP20
  • Human FABP2/I-FABP Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Human Plasma
    Verified Customer | Posted 02/08/2017
    Human FABP2/I-FABP Quantikine ELISA Kit DFBP20

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FAQs for Human FABP2/I-FABP Quantikine ELISA Kit

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  • Q: Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?

    A: A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

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