Human FABP2/I-FABP Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DFBP20
Control Products Available
Human FABP2/I-FABP ELISA Linearity
1 Image
Product Details
FAQs
Supplemental Products
Reviews (3)

Human FABP2/I-FABP Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL)
Sensitivity
6.21 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human FABP2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human FABP2/I-FABP Immunoassay is a 4.5 hour solid phase ELISA designed to measure FABP2 levels in cell culture supernates, cell lysates, serum, plasma, and urine. It contains E. coli-expressed recombinant human FABP2 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human FABP2 showed linear curves that were parallel to the standard curves obtained using the Quantikine Human FABP2 Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human FABP2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 77.5 207 425 80.5 216 446
Standard Deviation 3.14 7.24 12.4 8.94 17.4 26.6
CV% 4.1 3.5 2.9 11.1 8.1 6

Recovery

The recovery of human FABP2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 96-104
Cell Lysis Buffer (n=2) 83 81-85
EDTA Plasma (n=4) 97 85-102
Heparin Plasma (n=4) 95 96-104
Serum (n=4) 101 97-106
Urine (n=4) 90 85-95

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human FABP2 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human FABP2/I-FABP ELISA Linearity

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FABP2/I-FABP

Fatty acid binding proteins are small cytoplasmic lipid binding proteins that are expressed in a tissue specific manner. FABPs bind free fatty acids, cholesterol, and retinoids, and are involved in intracellular lipid transport. Circulating FABP levels are used as indicators of tissue damage. Some FABP polymorphisms have been associated with disorders of lipid metabolism and the development of atherosclerosis.

FABP2, also known as intestinal fatty acid binding protein (I-FABP or FABPI) and gut FABP (gFABP), is a member of the cytosolic fatty acid binding protein family. FABP2 mediates the absorption and intracellular transport of dietary long-chain fatty acids. Genetic variations of FABP2 are implicated in obesity and Type II diabetes. Human FABP2 shares 78%, 82%, and 86% amino acid sequence identity with mouse, rat, and canine FABP2, respectively.

Long Name:
Fatty Acid-Binding Protein 2/Intestinal FABP
Entrez Gene IDs:
2169 (Human); 25598 (Rat)
Alternate Names:
FABP2; FABPI; fatty acid binding protein 2, intestinal; Fatty acid-binding protein 2; fatty acid-binding protein, intestinal; IFABP; I-FABP; I-FABPMGC133132; Intestinal FABP; Intestinal-type fatty acid-binding protein
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs

Reviews for Human FABP2/I-FABP Quantikine ELISA Kit

Average Rating: 4.7 (Based on 3 Reviews)

5 Star
66.67%
4 Star
33.33%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human FABP2/I-FABP Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human FABP2/I-FABP Quantikine ELISA Kit
By Anonymous on 10/20/2017
Sample Tested: blood and respiratory

Human FABP2/I-FABP Quantikine ELISA Kit
By Anonymous on 09/07/2017
Sample Tested: Serum

The Quantikine ELISA kit worked as expected with low % CV's, and reproducible results.


Human FABP2/I-FABP Quantikine ELISA Kit
By Anonymous on 02/08/2017
Sample Tested: human plasma