Human FGF basic Antibody

(16 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human and bovine FGF basic in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant mouse FGF basic is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Bovine brain-derived FGF basic
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human FGF basic 146 aa (Catalog # 233-FB)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize FGF basic-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND50) is typically 0.08-0.4 µg/mL in the presence of 0.5 ng/mL Bovine FGF basic.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
FGF basic in Human Placenta. FGF basic was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human FGF basic Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑233‑NA) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to trophoblast cells in chorionic villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Cell Proliferation Induced by FGF basic and Neutralization by Human FGF basic Antibody. Bovine FGF basic (Catalog # 133-FB) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Bovine FGF basic (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF basic Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-233-NA). The ND50 is typically 0.08‑0.4 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF basic

FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35 ‑ 60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues, but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. A variety of forms of FGF basic are produced as a result of N-terminal extensions. These extensions affect localization of FGF basic in cellular compartments but do not affect biological activity. Binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. FGF acidic and basic appear to bind to the same high affinity receptors and show a similar range of biological activities. FGF basic stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. FGF basic induces neuron differentiation, survival, and regeneration. FGF basic also modulates embryonic development and differentiation. These observed in vitro functions of FGF basic suggest FGF basic may play a role in vivo in the modulation of such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally, FGF basic may participate in the production of a variety of pathological conditions resulting from excessive cell proliferation and excessive angiogenesis.

  • References:
    1. Coulier, F. et al. (1997) J. Mol. Evol. 44:43.
    2. Chen, C.H. et al. (2004) Curr. Vasc. Pharmacol. 2:33.
    3. Mohammadi, M. et al. (2005) Curr. Opin. Struct. Biol. 15:506.
    4. Fernig, D. et al. (1994) Prog. Growth Factor Res. 5:353.
  • Long Name:
    Fibroblast Growth Factor basic
  • Entrez Gene IDs:
    2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
  • Alternate Names:
    basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGF2AS; FGFBprostatropin; fibroblast growth factor 2 (basic); GFG1; HBGF-2; HBGH-2; heparin-binding growth factor 2; NUDT6; Prostatropin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Endothelial heparan sulfate 6-o-sulfation levels regulate angiogenic responses of endothelial cells to fibroblast growth factor 2 and vascular endothelial growth factor.
    Authors: Ferreras, Cristina, Rushton, Graham, Cole, Claire L, Babur, Muhammad, Telfer, Brian A, van Kuppevelt, Toin H, Gardiner, John M, Williams, Kaye J, Jayson, Gordon C, Avizienyte, Egle
    J Biol Chem, 2102;287(43):36132-46.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  2. Pericytes impair capillary blood flow and motor function after chronic spinal cord injury
    Authors: Y Li, AM Lucas-Osma, S Black, MV Bandet, MJ Stephens, R Vavrek, L Sanelli, KK Fenrich, AF Di Narzo, S Dracheva, IR Winship, K Fouad, DJ Bennett
    Nat. Med., 2017;0(0):.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  3. Tumor-associated B-cells induce tumor heterogeneity and therapy resistance
    Authors: R Somasundar, G Zhang, M Fukunaga-K, M Perego, C Krepler, X Xu, C Wagner, D Hristova, J Zhang, T Tian, Z Wei, Q Liu, K Garg, J Griss, R Hards, M Maurer, C Hafner, M Mayerhöfer, G Karanikas, A Jalili, V Bauer-Pohl, F Weihsengru, K Rappersber, J Koller, R Lang, C Hudgens, G Chen, M Tetzlaff, L Wu, DT Frederick, RA Scolyer, GV Long, M Damle, C Ellingswor, L Grinman, H Choi, BJ Gavin, M Dunagin, A Raj, N Scholler, L Gross, M Beqiri, K Bennett, I Watson, H Schaider, MA Davies, J Wargo, BJ Czerniecki, L Schuchter, D Herlyn, K Flaherty, M Herlyn, SN Wagner
    Nat Commun, 2017;8(1):607.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. Peroxynitrite upregulates angiogenic factors VEGF-A, BFGF, and HIF-1alpha in human corneal limbal epithelial cells.
    Authors: Ashki N, Chan A, Qin Y, Wang W, Kiyohara M, Lin L, Braun J, Wadehra M, Gordon L
    Invest Ophthalmol Vis Sci, 2014;55(3):1637-46.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Inhibition of PAI-1 induces neutrophil-driven neoangiogenesis and promotes tissue regeneration via production of angiocrine factors in mice.
    Blood, 2012;119(26):6382-93.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  6. The fibroblast-derived paracrine factor neuregulin-1 has a novel role in regulating the constitutive color and melanocyte function in human skin.
    Authors: Choi W, Wolber R, Gerwat W
    J. Cell. Sci., 2010;123(0):3102-11.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Pancreatic stellate cells: partners in crime with pancreatic cancer cells.
    Authors: Vonlaufen A, Joshi S, Qu C, Phillips PA, Xu Z, Parker NR, Toi CS, Pirola RC, Wilson JS, Goldstein D, Apte MV
    Cancer Res., 2008;68(7):2085-93.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  8. Temporally expressed PDGF and FGF-2 regulate embryonic coronary artery formation and growth.
    Authors: Tomanek RJ, Hansen HK, Christensen LP
    Arterioscler. Thromb. Vasc. Biol., 2008;28(7):1237-43.
    Species: Avian
    Sample Type: Whole Tissue
    Application: Neut
  9. A CXCL5- and bFGF-dependent effect of PDGF-B-activated fibroblasts in promoting trafficking and differentiation of bone marrow-derived mesenchymal stem cells.
    Authors: Nedeau AE, Bauer RJ, Gallagher K, Chen H, Liu ZJ, Velazquez OC
    Exp. Cell Res., 2008;314(11):2176-86.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  10. IFATS Series: FGF-2-induced HGF Secretion By Adipose-Derived Stromal Cells Inhibits Post-Injury Fibrogenesis Through A JNK-Dependent Mechanism.
    Authors: Suga H, Eto H, Shigeura T, Inoue K, Aoi N, Kato H, Nishimura S, Manabe I, Gonda K, Yoshimura K
    Stem Cells, 2008;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  11. Agrin, a novel basement membrane component in human and rat liver, accumulates in cirrhosis and hepatocellular carcinoma.
    Authors: Tatrai P, Dudas J, Batmunkh E, Máthé M, Zalatnai A, Schaff Z, Ramadori G, Kovalszky I
    Lab. Invest., 2006;86(11):1149-60.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  12. Positive correlation between estradiol and vascular endothelial growth factor but not fibroblast growth factor-2 in normal human breast tissue in vivo.
    Authors: Dabrosin C
    Clin. Cancer Res., 2005;11(22):8036-41.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  13. FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis.
    Authors: Steinberg Z, Myers C, Heim VM, Lathrop CA, Rebustini IT, Stewart JS, Larsen M, Hoffman MP
    Development, 2005;132(6):1223-34.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  14. Differential role of platelet granular mediators in angiogenesis.
    Authors: Brill A, Elinav H, Varon D
    Cardiovasc. Res., 2004;63(2):226-35.
    Species: Bovine
    Sample Type: Whole Cells
    Application: Neut
  15. Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis.
    Authors: Giuliani N, Colla S, Lazzaretti M, Sala R, Roti G, Mancini C, Bonomini S, Lunghi P, Hojden M, Genestreti G, Svaldi M, Coser P, Fattori PP, Sammarelli G, Gazzola GC, Almici C, Caramatti C, Mangoni L, Rizzoli V
    Blood, 2003;102(2):638-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  16. The anti-HIV cytokine midkine binds the cell surface-expressed nucleolin as a low affinity receptor.
    Authors: Said EA, Krust B, Nisole S, Svab J, Briand JP, Hovanessian AG
    J. Biol. Chem., 2002;277(40):37492-502.
    Species: Hamster
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 16 Citations
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