Human FGF basic Quantikine ELISA Kit

(59 citations)
(1 Review)
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL)
  • Sensitivity
    3 pg/mL
  • Assay Range
    10.0 - 640 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
  • Specificity
    Natural and recombinant human FGF basic.  This assay cross-reacts 40% with natural Bovine FGF-basic, 0.15% with natural Bovine FGF-acidic, 17% with recombinant Bovine FGF-basic, 18% with recombinant Mouse FGF-basic and 57% with recombinant Rat FGF-basic.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human FGF basic Immunoassay kit is a 4.5 hour solid phase ELISA designed to measure human FGF basic in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human FGF basic and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human FGF basic accurately. Results obtained using natural human FGF basic showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human FGF basic.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation2.83.27.13817.3


The recovery of FGF basic spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 108 94-119
EDTA Plasma (n=5) 103 90-124
Serum (n=5) 106 86-124
To assess the linearity of the assay, the following samples spiked with high concentrations of FGF basic were diluted with Calibrator Diluent and then assayed.
Human FGF basic Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: FGF basic
FGF basic (FGF-2) is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic binds to FGF R1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGF R1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.
    • Long Name
      Fibroblast Growth Factor basic
    • Entrez Gene IDs
      2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine);
    • Alternate Names
      basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF2; FGF-2; FGF2AS; FGFBprostatropin; fibroblast growth factor 2 (basic); GFG1; HBGF-2; HBGH-2; heparin-binding growth factor 2; NUDT6; Prostatropin;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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    Sample Type
    1. Mesenchymal progenitors aging highlights a miR-196 switch targeting HOXB7 as master regulator of proliferation and osteogenesis.
      Authors: Candini O, Spano C, Murgia A, Grisendi G, Veronesi E, Piccinno M, Ferracin M, Negrini M, Giacobbi F, Bambi F, Horwitz E, Conte P, Paolucci P, Dominici M
      Stem Cells, 2015;33(3):939-50.
      Species: Human
      Sample Type: Cell Culture Supernates
    2. In situ normoxia enhances survival and proliferation rate of human adipose tissue-derived stromal cells without increasing the risk of tumourigenesis.
      Authors: Choi, Jane Ru, Pingguan-Murphy, Belinda, Wan Abas, Wan Abu, Yong, Kar Wey, Poon, Chi Tat, Noor Azmi, Mat Aden, Omar, Siti Zaw, Chua, Kien Hui, Xu, Feng, Wan Safwani, Wan Kama
      PLoS ONE, 2015;10(1):e0115034.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. Breeding Strategy Determines Rupture Incidence in Post-Infarct Healing WARPing Cardiovascular Research.
      Authors: Deckx S, Carai P, Bateman J, Heymans S, Papageorgiou A
      PLoS ONE, 2015;10(9):e0139199.
      Species: Rat
      Sample Type: Cell Culture Supernates
    4. A novel decoy receptor fusion protein for FGF-2 potently inhibits tumour growth.
      Authors: Li D, Wei X, Xie K, Chen K, Li J, Fang J
      Br J Cancer, 2014;111(1):68-77.
      Species: Human
      Sample Type: recombinant protein
    5. Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
      Authors: Kinzebach S, Dietz L, Kluter H, Thierse H, Bieback K
      BMC Cell Biol, 2013;14(0):48.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. Human bone marrow-derived mesenchymal stem cells suppress human glioma growth through inhibition of angiogenesis.
      Authors: Ho I, Toh H, Ng W, Teo Y, Guo C, Hui K, Lam P
      Stem Cells, 2013;31(1):146-55.
      Species: Human
      Sample Type: Tissue Lysate
    7. The role of SDF-1-CXCR4/CXCR7 axis in biological behaviors of adipose tissue-derived mesenchymal stem cells in vitro.
      Authors: Li Q, Zhang A, Tao C, Li X, Jin P
      Biochem Biophys Res Commun, 2013;441(3):675-80.
      Species: Human
      Sample Type: Cell Culture Supernates
    8. Biomarkers for antitumor activity of bevacizumab in gastric cancer models.
      Authors: Yamashita-Kashima Y, Fujimoto-Ouchi K, Yorozu K, Kurasawa M, Yanagisawa M, Yasuno H, Mori K
      BMC Cancer, 2012;12(0):37.
      Species: Human
      Sample Type: Tissue Homogenates
    9. Differential expression of cancer associated proteins in breast milk based on age at first full term pregnancy.
      Authors: Qin W, Zhang K, Kliethermes B, Ruhlen RL, Browne EP, Arcaro KF, Sauter ER
      BMC Cancer, 2012;12(0):100.
      Species: Human
      Sample Type: Milk
    10. Cytomegalovirus impairs cytotrophoblast-induced lymphangiogenesis and vascular remodeling in an in vivo human placentation model.
      Authors: Tabata T, Petitt M, Fang-Hoover J, Rivera J, Nozawa N, Shiboski S, Inoue N, Pereira L
      Am J Pathol, 2012;181(5):1540-59.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
      Authors: Rauch C, Feifel E, Amann EM, Spotl HP, Schennach H, Pfaller W, Gstraunthaler G
      ALTEX, 2011;28(4):305-16.
      Species: Human
      Sample Type: Cell Lysates
    12. In vitro vascular tube formation testing as a tool for treatment individualisation in patients with cervical cancer.
      Authors: Landt S, Heidecke H, Korlach S, Reuter C, Schwidde I, Barinoff J, Thill M, Sehouli J, Kummel S
      Anticancer Res., 2011;31(8):2609-15.
      Species: Human
      Sample Type: Serum
    13. Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
      Authors: Li TS, Cheng K, Malliaras K, Matsushita N, Sun B, Marban L, Zhang Y, Marban E
      Cardiovasc. Res., 2011;89(1):157-65.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. Fibroblast growth factor receptors are components of autocrine signaling networks in head and neck squamous cell carcinoma cells.
      Authors: Marshall ME, Hinz TK, Kono SA, Singleton KR, Bichon B, Ware KE, Marek L, Frederick BA, Raben D, Heasley LE
      Clin. Cancer Res., 2011;17(15):5016-25.
      Species: Human
      Sample Type: Cell Lysates
    15. Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model.
      Authors: Brave SR, Eberlein C, Shibuya M, Wedge SR, Barry ST
      Angiogenesis, 2010;13(4):337-47.
      Species: Human
      Sample Type: Cell Culture Supernates
    16. Platelet-derived growth factor (PDGF)-PDGF receptor interaction activates bone marrow-derived mesenchymal stromal cells derived from chronic lymphocytic leukemia: implications for an angiogenic switch.
      Authors: Ding W, Knox TR, Tschumper RC
      Blood, 2010;116(16):2984-93.
      Species: Human
      Sample Type: Platelet-Poor Plasma
    17. Tumor-derived pancreatic stellate cells promote pancreatic cancer cell invasion through release of thrombospondin-2.
      Authors: Farrow B, Berger DH, Rowley D
      J. Surg. Res., 2009;156(1):155-60.
      Species: Human
      Sample Type: Cell Culture Supernates
    18. Lenalidomide plus prednisone results in durable clinical, histopathologic, and molecular responses in patients with myelofibrosis.
      Authors: Quintas-Cardama A, Kantarjian HM, Manshouri T, Thomas D, Cortes J, Ravandi F, Garcia-Manero G, Ferrajoli A, Bueso-Ramos C, Verstovsek S
      J. Clin. Oncol., 2009;27(28):4760-6.
      Species: Human
      Sample Type: Plasma
    19. Inhibitory effect of atractylenolide I on angiogenesis in chronic inflammation in vivo and in vitro.
      Authors: Wang C, Duan H, He L
      Eur. J. Pharmacol., 2009;612(1):143-52.
      Species: Mouse
      Sample Type: Air Pouch Exudate
    20. A urinary biomarker profile for children with HIV-associated renal diseases.
      Authors: Soler-Garcia AA, Rakhmanina NY, Mattison PC, Ray PE
      Kidney Int., 2009;76(2):207-14.
      Species: Human
      Sample Type: Urine
    21. Clinical correlations and prognostic relevance of HGF, VEGF AND FGF expression in Brazilian patients with non-Hodgkin lymphoma.
      Authors: Etto L, Lacerda E, Baiocchi O, Silva V, Dalboni M, Alves A, Silva M, Vettore A, Colleoni G
      Leuk. Lymphoma, 2008;49(2):257-64.
      Species: Human
      Sample Type: Serum
    22. Elevated fibroblast growth factor-2 increases tumor necrosis factor-alpha induced endothelial cell death in high glucose.
      Authors: Clyne AM, Zhu H, Edelman ER
      J. Cell. Physiol., 2008;217(1):86-92.
      Species: Porcine
      Sample Type: Whole Cells
    23. Connexin43 knockdown accelerates wound healing but inhibits mesenchymal transition after corneal endothelial injury in vivo.
      Authors: Nakano Y, Oyamada M, Dai P, Nakagami T, Kinoshita S, Takamatsu T
      Invest. Ophthalmol. Vis. Sci., 2008;49(1):93-104.
      Species: Rat
      Sample Type: Aqueous humor
    24. Observations on the microvasculature of bone defects filled with biodegradable nanoparticulate hydroxyapatite.
      Authors: Kilian O, Wenisch S, Karnati S, Baumgart-Vogt E, Hild A, Fuhrmann R, Jonuleit T, Dingeldein E, Schnettler R, Franke RP
      Biomaterials, 2008;29(24):3429-37.
      Species: Porcine
      Sample Type: Plasma
    25. Fibroblast growth factor-2 expression is altered in lambs with increased pulmonary blood flow and pulmonary hypertension.
      Authors: Wedgwood S, Devol JM, Grobe A, Benavidez E, Azakie A, Fineman JR, Black SM
      Pediatr. Res., 2007;61(1):32-6.
      Species: Ovine
      Sample Type: Cell Culture Supernates
    26. Electronegative LDL impairs vascular endothelial cell integrity in diabetes by disrupting fibroblast growth factor 2 (FGF2) autoregulation.
      Authors: Lu J, Jiang W, Yang JH, Chang PY, Walterscheid JP, Chen HH, Marcelli M, Tang D, Lee YT, Liao WS, Yang CY, Chen CH
      Diabetes, 2007;57(1):158-66.
      Species: Bovine
      Sample Type: Cell Culture Supernates
    27. Selective cyclooxygenase-2 inhibitor downregulates the paracrine epithelial-mesenchymal interactions of growth in scirrhous gastric carcinoma.
      Authors: Yashiro M, Nakazawa K, Tendo M, Kosaka K, Shinto O, Hirakawa K
      Int. J. Cancer, 2007;120(3):686-93.
      Species: Human
      Sample Type: Cell Culture Supernates
    28. Angiogenic growth factors in maternal and fetal serum in pregnancies complicated by intrauterine growth restriction.
      Authors: Wallner W, Sengenberger R, Strick R, Strissel PL, Meurer B, Beckmann MW, Schlembach D
      Clin. Sci., 2007;112(1):51-7.
      Species: Human
      Sample Type: Serum
    29. Sca-1(+) Hematopoietic Cell-based Gene Therapy with a Modified FGF-2 Increased Endosteal/Trabecular Bone Formation in Mice.
      Authors: Hall SL, Lau KH, Chen ST, Wergedal JE, Srivastava A, Klamut H, Sheng MH, Gridley DS, Mohan S, Baylink DJ
      Mol. Ther., 2007;15(10):1881-9.
      Species: Mouse
      Sample Type: Serum
    30. Targeted deletion of CCR2 impairs deep vein thombosis resolution in a mouse model.
      Authors: Henke PK, Pearce CG, Moaveni DM, Moore AJ, Lynch EM, Longo C, Varma M, Dewyer NA, Deatrick KB, Upchurch GR, Wakefield TW, Hogaboam C, Kunkel SL
      J. Immunol., 2006;177(5):3388-97.
      Species: Mouse
      Sample Type: Tissue Homogenates
    31. Quantitative assessment of growth factors in reaming aspirate, iliac crest, and platelet preparation.
      Authors: Herrmann S, Green J, Weber T, Scharfenberger A
      Bone, 2006;39(5):1156-63.
      Species: Human
      Sample Type: Tissue Homogenates
    32. Insulin-like growth factor binding protein-4 (IGFBP-4) is a novel anti-angiogenic and anti-tumorigenic mediator secreted by dibutyryl cyclic AMP (dB-cAMP)-differentiated glioblastoma cells.
      Authors: Moreno MJ, Ball M, Andrade MF, McDermid A, Stanimirovic DB
      Glia, 2006;53(8):845-57.
      Species: Human
      Sample Type: Cell Culture Supernates
    33. Serum levels of syndecan-1 in B-cell chronic lymphocytic leukemia: correlation with the extent of angiogenesis and disease-progression risk in early disease.
      Authors: Molica S, Vitelli G, Mirabelli R, Digiesu G, Giannarelli D, Cuneo A, Ribatti D, Vacca A
      Leuk. Lymphoma, 2006;47(6):1034-40.
      Species: Human
      Sample Type: Serum
    34. A phase I biological and pharmacologic study of the heparanase inhibitor PI-88 in patients with advanced solid tumors.
      Authors: Basche M, Gustafson DL, Holden SN, O'Bryant CL, Gore L, Witta S, Schultz MK, Morrow M, Levin A, Creese BR, Kangas M, Roberts K, Nguyen T, Davis K, Addison RS, Moore JC, Eckhardt SG
      Clin. Cancer Res., 2006;12(18):5471-80.
      Species: Human
      Sample Type: Urine
    35. Relation of various plasma growth factor levels in patients with stable angina pectoris and total occlusion of a coronary artery to the degree of coronary collaterals.
      Authors: Briguori C, Testa U, Colombo A, Petrucci E, Condorelli G, Airoldi F, Peschle C, Condorelli G
      Am. J. Cardiol., 2005;97(4):472-6.
      Species: Human
      Sample Type: Serum
    36. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
      Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
      J. Immunol., 2005;175(9):6177-89.
      Species: Human
      Sample Type: Cell Culture Supernates
    37. Alpha-tocopheryl succinate inhibits malignant mesothelioma by disrupting the fibroblast growth factor autocrine loop: mechanism and the role of oxidative stress.
      Authors: Stapelberg M, Gellert N, Swettenham E, Tomasetti M, Witting PK, Procopio A, Neuzil J
      J. Biol. Chem., 2005;280(27):25369-76.
      Species: Human
      Sample Type: Cell Culture Supernates
    38. Repair of articular cartilage defect by intraarticular administration of basic fibroblast growth factor gene, using adeno-associated virus vector.
      Authors: Hiraide A, Yokoo N, Xin KQ, Okuda K, Mizukami H, Ozawa K, Saito T
      Hum. Gene Ther., 2005;16(12):1413-21.
      Species: Rat
      Sample Type: Cell Culture Supernates
    39. Functional and structural remodeling of the myocardial microvasculature in early experimental hypertension.
      Authors: Rodriguez-Porcel M, Zhu XY, Chade AR, Amores-Arriaga B, Caplice NM, Ritman EL, Lerman A, Lerman LO
      Am. J. Physiol. Heart Circ. Physiol., 2005;290(3):H978-84.
      Species: Porcine
      Sample Type: Tissue Homogenates
    40. Tumor necrosis factor alpha and adiponectin in bone marrow interstitial fluid from patients with acute myeloid leukemia inhibit normal hematopoiesis.
      Authors: Iversen PO, Wiig H
      Clin. Cancer Res., 2005;11(19):6793-9.
      Species: Human
      Sample Type: Plasma
    41. A potential pro-angiogenic cell therapy with human placenta-derived mesenchymal cells.
      Authors: Nishishita T, Ouchi K, Zhang X, Inoue M, Inazawa T, Yoshiura K, Kuwabara K, Nakaoka T, Watanabe N, Igura K, Takahashi TA, Yamashita N
      Biochem. Biophys. Res. Commun., 2004;325(1):24-31.
      Species: Human
      Sample Type: Cell Lysates
    42. Intravenous administration of marrow stromal cells (MSCs) increases the expression of growth factors in rat brain after traumatic brain injury.
      Authors: Mahmood A, Lu D, Chopp M
      J. Neurotrauma, 2004;21(1):33-9.
      Species: Rat
      Sample Type: Tissue Homogenates
    43. Transplantation of transduced retinal pigment epithelium in rats.
      Authors: Saigo Y, Abe T, Hojo M, Tomita H, Sugano E, Tamai M
      Invest. Ophthalmol. Vis. Sci., 2004;45(6):1996-2004.
      Species: Rat
      Sample Type: Cell Culture Supernates
    44. Diabetes can alter the signal transduction pathways in the lens of rats.
      Authors: Zatechka DS, Kador PF, Garcia-Castineiras S, Lou MF
      Diabetes, 2003;52(4):1014-22.
      Species: Rat
      Sample Type: Vitreous Humor
    45. Low-density lipoprotein in hypercholesterolemic human plasma induces vascular endothelial cell apoptosis by inhibiting fibroblast growth factor 2 transcription.
      Authors: Chen CH, Jiang T, Yang JH, Jiang W, Lu J, Marathe GK, Pownall HJ, Ballantyne CM, McIntyre TM, Henry PD, Yang CY
      Circulation, 2003;107(16):2102-8.
      Species: Bovine
      Sample Type: Cell Lysates
    46. Have urinary levels of the angiogenic factors, basic fibroblast growth factor and vascular endothelial growth factor, a prognostic value in childhood acute lymphoblastic leukaemia?
      Authors: Schneider P, Vasse M, Legrand E, Callat MP, Vannier JP
      Br. J. Haematol., 2003;122(1):163-4.
      Species: Human
      Sample Type: Urine
    47. beta-Catenin regulates vascular endothelial growth factor expression in colon cancer.
      Authors: Easwaran V, Lee SH, Inge L, Guo L, Goldbeck C, Garrett E, Wiesmann M, Garcia PD, Fuller JH, Chan V, Randazzo F, Gundel R, Warren RS, Escobedo J, Aukerman SL, Taylor RN, Fantl WJ
      Cancer Res., 2003;63(12):3145-53.
      Species: Mouse
      Sample Type: Serum
    48. High basic fibroblast growth factor levels in nipple aspirate fluid are correlated with breast cancer.
      Authors: Hsiung R, Zhu W, Klein G, Qin W, Rosenberg A, Park P, Rosato E, Sauter E
      Cancer J, 2002;8(4):303-10.
      Species: Human
      Sample Type: Nipple aspirate fluid
    49. Basic fibroblast growth factor in human saliva decreases with aging.
      Authors: Westermark A, Pyykko I, Magnusson M, Ishizaki H, Jantti P, van Setten G
      Laryngoscope, 2002;112(5):887-9.
      Species: Human
      Sample Type: Saliva
    50. Retinoscleral control of scleral remodelling in refractive development: a role for endogenous FGF-2?
      Authors: Gentle A, McBrien NA
      Cytokine, 2002;18(6):344-8.
      Species: Avian - Tree Shrew
      Sample Type: Tissue Homogenates
    51. Increase in gap junctional intercellular communication by high molecular weight hyaluronic acid associated with fibroblast growth factor 2 and keratinocyte growth factor production in normal human dermal fibroblasts.
      Authors: Park JU, Tsuchiya T
      Tissue Eng., 2002;8(3):419-27.
      Species: Human
      Sample Type: Cell Culture Supernates
    52. Sugar-induced modification of fibroblast growth factor 2 reduces its angiogenic activity in vivo.
      Authors: Facchiano F, Lentini A, Fogliano V, Mancarella S, Rossi C, Facchiano A, Capogrossi MC
      Am. J. Pathol., 2002;161(2):531-41.
      Species: Bovine
      Sample Type: Cell Culture Supernates
    53. Circadian variations of transforming growth factor-beta2 and basic fibroblast growth factor in the rabbit aqueous humor.
      Authors: Liu JH
      Curr. Eye Res., 2002;24(1):75-80.
      Species: Rabbit
      Sample Type: Aqueous humor
    54. AF5, a CNS cell line immortalized with an N-terminal fragment of SV40 large T: growth, differentiation, genetic stability, and gene expression.
      Authors: Truckenmiller ME, Vawter MP, Zhang P, Conejero-Goldberg C, Dillon-Carter O, Morales N, Cheadle C, Becker KG, Freed WJ
      Exp. Neurol., 2002;175(0):318.
      Species: Rat
      Sample Type: Cell Culture Supernates
    55. Cyclic mechanical stretching modulates secretion pattern of growth factors in human tendon fibroblasts.
      Authors: Skutek M, van Griensven M, Zeichen J, Brauer N, Bosch U
      Eur. J. Appl. Physiol., 2001;86(1):48-52.
      Species: Human
      Sample Type: Cell Culture Supernates
    56. Angiogenic balance in human melanoma: expression of VEGF, bFGF, IL-8, PDGF and angiostatin in relation to vascular density of xenografts in vivo.
      Authors: Westphal JR, Van't Hullenaar R, Peek R, Willems RW, Crickard K, Crickard U, Askaa J, Clemmensen I, Ruiter DJ, De Waal RM
      Int. J. Cancer, 2000;86(6):768-76.
      Species: Human
      Sample Type: Cell Culture Supernates
    57. Intestinal mucosal secretion of basic fibroblast growth factor in patients with ulcerative colitis.
      Authors: Thorn M, Raab Y, Larsson A, Gerdin B, Hallgren R
      Scand. J. Gastroenterol., 2000;35(4):408-12.
      Species: Human
      Sample Type: perfusion fluid
    58. Growth factors and proinflammatory cytokines in the renal involvement of POEMS syndrome.
      Authors: Soubrier M, Sauron C, Souweine B, Larroche C, Wechsler B, Guillevin L, Piette JC, Rousset H, Deteix P
      Am. J. Kidney Dis., 1999;34(4):633-8.
      Species: Human
      Sample Type: Serum
    59. Hypoxia-induced expression of VEGF is reversible in myocardial vascular smooth muscle cells.
      Authors: Gu JW, Adair TH
      Am. J. Physiol., 1997;273(2):H628-33.
      Species: Canine
      Sample Type: Cell Culture Supernates
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