Human FOLR4 Antibody

MAB6328 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6328
MAB6328-SP

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Human FOLR4 Antibody in Immunohistochemistry

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FOLR4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FOLR4 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 821221

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human FOLR4
Glu134-Trp210
Accession # A6ND01

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry

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FOLR4 in Human Melanoma. FOLR4 was detected in immersion fixed paraffin-embedded sections of human melanoma using Rat Anti-Human FOLR4 Monoclonal Antibody (Catalog # MAB6328) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FOLR4

FOLR4 (Folate Receptor 4), also known as FR4, Folate Receptor δ, and Folate Binding Protein 3, is a 34‑36 kDa member of the folate receptor family of molecules. Based on mouse, it appears to be a marker for subsets of T cells. In particular, and in conjunction with CD25, FR4^hiCD25^hi expression defines T reg cells, FR4^loCD25⁺ expression pattern identifies effector memory T cells, and a FR4^hiCD25^- combination characterizes central memory T cells. Mature human FOLR4 is 225 amino acids (aa) in length (aa 20‑244). It contains virtually no identifiable motifs. There is one potential splice variant that shows a five aa substitution for aa 111‑116. Over aa 134‑220, human FOLR4 shares 72% aa identity with mouse FOLR4.

Long Name

Folate Receptor 4

Entrez Gene IDs

390243 (Human); 64931 (Mouse); 690037 (Rat)

Alternate Names

folate receptor 4 (delta) homolog (mouse); Folbp3; FOLR4; FR-delta; LOC390243; probable folate receptor delta