Human FOLR4 Antibody

MAB6328 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6328
MAB6328-SP

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Human FOLR4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FOLR4 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 821221

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human FOLR4
Glu134-Trp210
Accession # A6ND01

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry

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FOLR4 in Human Melanoma. FOLR4 was detected in immersion fixed paraffin-embedded sections of human melanoma using Rat Anti-Human FOLR4 Monoclonal Antibody (Catalog # MAB6328) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FOLR4

FOLR4 (Folate Receptor 4), also known as FR4, Folate Receptor δ, and Folate Binding Protein 3, is a 34‑36 kDa member of the folate receptor family of molecules. Based on mouse, it appears to be a marker for subsets of T cells. In particular, and in conjunction with CD25, FR4^hiCD25^hi expression defines T reg cells, FR4^loCD25⁺ expression pattern identifies effector memory T cells, and a FR4^hiCD25^- combination characterizes central memory T cells. Mature human FOLR4 is 225 amino acids (aa) in length (aa 20‑244). It contains virtually no identifiable motifs. There is one potential splice variant that shows a five aa substitution for aa 111‑116. Over aa 134‑220, human FOLR4 shares 72% aa identity with mouse FOLR4.

Long Name

Folate Receptor 4

Entrez Gene IDs

390243 (Human); 64931 (Mouse); 690037 (Rat)

Alternate Names

folate receptor 4 (delta) homolog (mouse); Folbp3; FOLR4; FR-delta; LOC390243; probable folate receptor delta