Human FRAT2 Antibody

Catalog # Availability Size / Price Qty
AF7980
AF7980-SP
FRAT2 in NTera‑2 Human Cell Line.
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Human FRAT2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FRAT2 in direct ELISAs.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FRAT2
Ser31-Leu233 (Ala83Thr)
Accession # O75474
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry FRAT2 in NTera‑2 Human Cell Line. View Larger

FRAT2 in NTera‑2 Human Cell Line. FRAT2 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line cultured with (left panels) and without (right panels) 10μM retinoic acid for 4 days using Sheep Anti-Human FRAT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7980) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panels; Catalog # NL010) and counterstained with DAPI (blue, lower panels). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FRAT2

FRAT2 (Frequently Rearranged in Advanced T-cell lymphomas-2; also GSK-3-binding protein FRAT2) is an intracellular member of the GSK-3-binding protein family. Although its predicted MW is 28 kDa, due to its highly acidic nature, it runs anomalously at 35 kDa in SDS-PAGE. It is widely expressed, and plays a key role in the regulation of the Wnt signaling pathway. Typically, in the absence of stimulation, GSK-3 beta and beta -catenin associate with Axin, where GSK-3 beta phosphorylates beta -catenin, leading to its turnover. Upon Wnt stimulation, GSK-3 beta phosphorylation of beta -catenin is decoupled, resulting in beta -catenin mediated gene activation. FRAT2 has the same effect as Wnt stimulation on GSK-3 beta activity, serving as a non-Wnt activator of beta -catenin. It does so by promoting the dissociation of GSK-3 beta from axin. It also interacts with Diversin, a participant in JNK signaling. This suggests that FRAT2 plays a role in both Wnt/ beta -catenin and PCP signaling pathways. Human FRAT2 is 233 amino acids (aa) in length. It contains an N-terminal acidic domain (aa 1-54), a Pro-rich segment (aa 60-107) and a GSK-3 beta binding region (aa 171-196). Over aa 31‑233, human FRAT2 shares 73% aa sequence identity with both mouse FRAT2 and human FRAT1.

Long Name
Frequently Rearranged in Advanced T-cell Lymphomas 2
Entrez Gene IDs
23401 (Human); 212398 (Mouse)
Alternate Names
FRAT2; frequently rearranged in advanced T-cell lymphomas 2FRAT-2; GSK-3 binding protein FRAT2; GSK-3-binding protein FRAT2; MGC10562

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