Human Free IGF-I/IGF-1 ELISA Kit

Catalog # Availability Size / Price Qty
DFG100B
Control Products Available
Human Free IGF-I ELISA Standard Curve
2 Images
Product Details
Citations (3)
FAQs
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Human Free IGF-I/IGF-1 ELISA Kit Summary

Assay Length
1.75 hours
Sample Type & Volume Required Per Well
EDTA Plasma (50 uL)
Sensitivity
0.006 ng/mL
Assay Range
0.1 - 6 ng/mL (EDTA Plasma)
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Free IGF-I enzyme linked immunosorbent assay (ELISA) is a 1.75 hour solid-phase ELISA designed to measure human Free IGF-I in EDTA Plasma. It contains E. coli-expressed recombinant human IGF-I and has been shown to accurately quantitate the recombinant factor.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in thirty-two separate assays to assess inter-assay precision

EDTA Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 32 32 32
Mean 0.669 2.04 4.53 0.677 2.03 4.29
Standard Deviation 0.014 0.041 0.135 0.038 0.088 0.199
CV% 2.1 2 3 5.6 4.3 4.6

Data Examples

Human Free IGF-I ELISA Standard Curve

Human Free IGF-I Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGF-I/IGF-1

IGF-1 (previously called somatomedin C) is a 7.6 kDa, 70 amino acid (aa) polypeptide with three internal disulfide bonds. The sequence of human IGF-1 is identical to that of bovine and porcine IGF-1, and it is 70% identical to human IGF-II. IGF-1 is a single-chain molecule with about 50% identity to the sequences of the A- and B-chains of human insulin. 


While IGF-1 has very complex functions, it appears largely to mediate the actions of growth hormone. Thus, IGF-1 is important in prenatal development, growth to adulthood and metabolic control. It induces amino acid uptake, protein synthesis and glucose utilization. It is an important mitogen and regulator of the cell cycle and apoptosis. IGF-1 is produced primarily by hepatocytes, serving an endocrine function. It is also produced by many other cells, where it may act in an autocrine or paracrine manner. Serum levels of IGF-1 have been reported to increase from birth to puberty, followed by a slow decline through adulthood. 

There are two receptors for the IGFs; type I IGF receptor, which signals through a tyrosine kinase, and type II IGF receptor, which is identical to the mannose-6-phosphate receptor and may not signal. The action of IGF on its receptors is very complex, with control by at least six IGF-binding proteins (IGFBP-1 through IGFBP-6). IGFBP-3 binds over 90% of the total IGF in serum in a complex of IGF, IGFBP and an acid-labile subunit. This ternary complex greatly stabilizes IGF in the circulation, changing the half life from minutes to hours. In addition, IGFBPs modulate the action of IGF on the membrane receptors. Adding to the complexity is a family of proteases that act on IGFBPs, modifying their affinity for IGF or completely eliminating the IGFBPs. The interactions of IGF, IGFBP, IGFBP proteases, and IGF receptors are referred to as the IGF axis. The IGF axis affects many primary physiological and pathological processes, including development, growth, metabolic regulation, tumorigenesis, atherosclerosis and angiogenesis.

Long Name:
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Entrez Gene IDs:
3479 (Human); 16000 (Mouse); 24482 (Rat)
Alternate Names:
IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Free IGF-I/IGF-1 ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Trio, a novel bovine high fecundity allele: III. Acquisition of dominance and ovulatory capacity at a smaller follicle size
    Authors: A García-Gue, AMO Canavessi, PLJM Jr, MA Mezera, R Sartori, BW Kirkpatric, MC Wiltbank
    Biol. Reprod., 2018;0(0):.
    Species: Bovine
    Sample Types: Follicular Fluid
  2. Detection of Placental Proteomes at Different Uterine Positions in Large White and Meishan Gilts on Gestational Day 90
    PLoS ONE, 2016;11(12):e0167799.
    Species: Porcine
    Sample Types: Serum
  3. IGFBP-2 inhibits adipogenesis and lipogenesis in human visceral, but not subcutaneous, adipocytes.
    Authors: Yau S, Russo V, Clarke I, Dunshea F, Werther G, Sabin M
    Int J Obes (Lond), 2015;39(5):770-81.
    Species: Human
    Sample Types: Cell Culture Supernates

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