FTO (FATSO; also Fat mass and obesity associated gene) is a 58 kDa member of the AlkB family of Ferrous/2-Oxoglutarate-dependent oxidative DNA/RNA demethylases. It is found the nucleus, and apparently serves to protect single stranded RNA and DNA against methylation. Although FTO is ubiquitously expressed, it has high levels in neurons that are involved in regulating energy intake, and is often found to colocalize with oxytocin. Evidence suggests it promotes feeding behavior. Human FTO is 505 amino acids (aa) in length. It contains one dioxygenase catalytic region (aa 32-327) and a C-terminal FTO domain (aa 329-500). There are three potential isoform variants. One shows a ten aa substitution for aa 1-455, a second contains a 35 aa substitution for aa 1-413, and a third utilizes an alternative start site at Met400. Over aa 1-178, human FTO shares 89% aa identity with mouse FTO.
Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Met1-Arg178
Accession # Q9C0B1
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human FTO Antibody
Detection of Human FTO by Western Blot.
Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, A172 human glioblastoma cell line, U-118-MG human glioblastoma/astrocytoma cell line, and HEC-1-B human endometrial adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human FTO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7208) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FTO at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Applications for Human FTO Antibody
Western Blot
Sample: SH‑SY5Y human neuroblastoma cell line, A172 human glioblastoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, and HEC‑1‑B human endometrial adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: FTO
Long Name
Alternate Names
Gene Symbol
UniProt
Additional FTO Products
Product Documents for Human FTO Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human FTO Antibody
For research use only
Citations for Human FTO Antibody
Powered by Bioz
Customer Reviews for Human FTO Antibody
There are currently no reviews for this product. Be the first to review Human FTO Antibody and earn rewards!
Have you used Human FTO Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars