Human FTO Antibody

Catalog # Availability Size / Price Qty
Detection of Human FTO by Western Blot.
1 Image
Product Details
Citations (1)
Supplemental Products

Human FTO Antibody Summary

Species Reactivity
Detects human FTO in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human FTO
Accession # Q9C0B1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human FTO antibody by Western Blot. View Larger

Detection of Human FTO by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, A172 human glioblastoma cell line, U-118-MG human glioblastoma/astrocytoma cell line, and HEC-1-B human endometrial adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human FTO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7208) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FTO at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FTO

FTO (FATSO; also Fat mass and obesity associated gene) is a 58 kDa member of the AlkB family of Ferrous/2-Oxoglutarate-dependent oxidative DNA/RNA demethylases. It is found the nucleus, and apparently serves to protect single stranded RNA and DNA against methylation. Although FTO is ubiquitously expressed, it has high levels in neurons that are involved in regulating energy intake, and is often found to colocalize with oxytocin. Evidence suggests it promotes feeding behavior. Human FTO is 505 amino acids (aa) in length. It contains one dioxygenase catalytic region (aa 32-327) and a C-terminal FTO domain (aa 329-500). There are three potential isoform variants. One shows a ten aa substitution for aa 1-455, a second contains a 35 aa substitution for aa 1-413, and a third utilizes an alternative start site at Met400. Over aa 1-178, human FTO shares 89% aa identity with mouse FTO.

Long Name
Fat Mass and Obesity Associated
Entrez Gene IDs
79068 (Human); 26383 (Mouse); 291905 (Rat)
Alternate Names
Alpha-ketoglutarate-dependent dioxygenase FTO; EC 1.14.11.-; fat mass and obesity associated; Fat mass and obesity-associated protein; FTO; KIAA1752protein fto; MGC5149

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Citation for Human FTO Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Kaposi's Sarcoma-Associated Herpesvirus Utilizes and Manipulates RNA N(6)-Adenosine Methylation to Promote Lytic Replication
    Authors: F Ye, ER Chen, TW Nilsen
    J. Virol., 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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