Detection of Human FTO by Western Blot.
Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line, A172 human glioblastoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, and HEC‑1‑B human endometrial adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human FTO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7208) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FTO at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
FTO (FATSO; also Fat mass and obesity associated gene) is a 58 kDa member of the AlkB family of Ferrous/2-Oxoglutarate-dependent oxidative DNA/RNA demethylases. It is found the nucleus, and apparently serves to protect single stranded RNA and DNA against methylation. Although FTO is ubiquitously expressed, it has high levels in neurons that are involved in regulating energy intake, and is often found to colocalize with oxytocin. Evidence suggests it promotes feeding behavior. Human FTO is 505 amino acids (aa) in length. It contains one dioxygenase catalytic region (aa 32-327) and a C-terminal FTO domain (aa 329-500). There are three potential isoform variants. One shows a ten aa substitution for aa 1-455, a second contains a 35 aa substitution for aa 1-413, and a third utilizes an alternative start site at Met400. Over aa 1-178, human FTO shares 89% aa identity with mouse FTO.
Fat Mass and Obesity Associated
Entrez Gene IDs:
79068 (Human); 26383 (Mouse); 291905 (Rat)
Alpha-ketoglutarate-dependent dioxygenase FTO; EC 1.14.11.-; fat mass and obesity associated; Fat mass and obesity-associated protein; FTO; KIAA1752protein fto; MGC5149
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