Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Galanin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Galanin
Ala20-Ser123,
Accession # P22466

Specificity

Detects human Galanin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Galanin Antibody

Detection of Human Galanin antibody by Western Blot.

Detection of Human Galanin by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Galanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5854) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Galanin at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Human Galanin antibody by Simple WesternTM.

Detection of Human Galanin by Simple WesternTM.

Simple Western lane view shows lysates of SH-SY5Y human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galanin at approximately 13 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Galanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5854) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Galanin Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line

Western Blot

1 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Galanin

Galanin (Glycine-Alanine, representing the N-and C-terminal amino acids in bovine Galanin; also GAL) is a 3 kDa (predicted), secreted member of the Galanin family of peptides. It is co-expressed with differing neuropeptides in a variety of neuron cell types. Galanin affects multiple metabolic processes by binding to one of three GPCRs. GalR1 blocks insulin secretion, GalR2 initiates neurogenesis and GalR3 influences addictive behavior. The human Galanin proform is 10‑12 kDa in size (predicted) and 104 amino acids (aa) in length. Proteolytic processing generates a 30 aa mature Galanin peptide (aa 33‑62), plus a phosphorylated (Ser117), C-terminal 59 aa GMAP fragment that is apparently involved in the processing of noxious stimuli. Once secreted, Galanin can undergo additional proteolytic degradation. Over aa 20‑123, the human Galanin proform shares 72% aa identity with mouse Galanin proform.

Alternate Names

GAL, GALN

Entrez Gene IDs

51083 (Human); 14419 (Mouse); 29141 (Rat)

Gene Symbol

GAL

UniProt

P22466 (Human)

Additional Galanin Products

Product Documents for Human Galanin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Galanin Antibody

For research use only

Citations for Human Galanin Antibody

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