Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot, Flow Cytometry, ELISA Development (Detection)

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Galectin-9 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Galectin-9

Specificity

Detects human Galectin-9 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) Galectin-3 and rhGalectin-4 is observed and less than 2% cross-reactivity with rhGalectin-1, rhGalectin-2, rhGalectin-7, and rhGalectin-8 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Galectin‑9 Biotinylated Antibody

Galectin‑9 in U937 Human Cell Line.

Galectin‑9 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Goat Anti-Human Galectin‑9 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2045) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Galectin‑9 Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human Galectin‑9

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Galectin-9

Galectin-9 (also known as Ecalectin and UAT) is a 40 kDa beta -galactoside binding lectin that belongs to a tandem repeat group of Galectins. Activated cells such as T cells, PMNs, astrocytes, fibroblasts and endothelial cells can express Galectin-9, but its release requires select MMPs. Human to mouse, there is 69% aa sequence identity for Galectin-9.

Alternate Names

Ecalectin, GAL9, Galectin9, LGALS9

Entrez Gene IDs

3965 (Human); 16859 (Mouse)

Gene Symbol

LGALS9

Additional Galectin-9 Products

Product Documents for Human Galectin‑9 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Galectin‑9 Biotinylated Antibody

For research use only

Citations for Human Galectin‑9 Biotinylated Antibody

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Protocols

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