Human Galectin-9 Antibody

(11 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Galectin-9 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Galectin-3 and rhGalectin-4 is observed and less than 2% cross-reactivity with rhGalectin-1, rhGalectin-2, rhGalectin-7, and rhGalectin-8 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Galectin-9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    0.3-15 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Galectin‑9 by Western Blot. Western blot shows conditioned media from HT‑29 human colon adenocarcinoma cell line, human peripheral blood lymphocytes (PBL), and human dendritic cells
untreated (-) or treated (+) with 10 µg/mL PHA for 6 days. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2045) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Galectin‑9 at approximately 36 and 40 kDa. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Galectin‑9 in Human PBMCs. Galectin‑9 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with Calcium Ionomycin and PMA using Goat Anti-Human Galectin‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2045) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunohistochemistry
Galectin‑9 in Human Colon. Galectin‑9 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human Galectin‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2045) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galectin-9
Galectin-9 (also known as Ecalectin and UAT) is a 40 kDa beta -galactosidase binding lectin that belongs to a tandem repeat group of Galectins. Activated cells such as T cells, PMNs, astrocytes, fibroblasts and endothelial cells can express Galectin-9, but its release requires select MMPs. Human to mouse, there is 69% aa sequence identity for Galectin-9.
  • Entrez Gene IDs:
    3965 (Human); 16859 (Mouse)
  • Alternate Names:
    Ecalectin; GAL9; gal-9; galectin 9; Galectin9; Galectin-9; HUAT; lectin, galactoside-binding, soluble, 9; LGALS9; LGALS9A; MGC117375; MGC125973; MGC125974; Tumor antigen HOM-HD-21; urate transporter/channel protein
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Activation of Human Basophils by A549 Lung Epithelial Cells Reveals a Novel IgE-Dependent Response Independent of Allergen
    Authors: JT Schroeder, AP Bieneman
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Tim-3 Is Upregulated in NK Cells during Early Pregnancy and Inhibits NK Cytotoxicity toward Trophoblast in Galectin-9 Dependent Pathway.
    Authors: Sun J, Yang M, Ban Y, Gao W, Song B, Wang Y, Zhang Y, Shao Q, Kong B, Qu X
    PLoS ONE, 2016;11(1):e0147186.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  3. Galectin-1, -3 and -9 Expression and Clinical Significance in Squamous Cervical Cancer.
    Authors: Punt S, Thijssen V, Vrolijk J, de Kroon C, Gorter A, Jordanova E
    PLoS ONE, 2015;10(6):e0129119.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. Graves' disease is associated with a defective expression of the immune regulatory molecule galectin-9 in antigen-presenting dendritic cells.
    Authors: Leskela S, Serrano A, de la Fuente H, Rodriguez-Munoz A, Ramos-Levi A, Sampedro-Nunez M, Sanchez-Madrid F, Gonzalez-Amaro R, Marazuela M
    PLoS ONE, 2015;10(4):e0123938.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Galectin expression profiling identifies galectin-1 and Galectin-9Delta5 as prognostic factors in stage I/II non-small cell lung cancer.
    Authors: Schulkens I, Heusschen R, van den Boogaart V, van Suylen R, Dingemans A, Griffioen A, Thijssen V
    PLoS ONE, 2014;9(9):e107988.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  6. Human cytomegalovirus upregulates expression of the lectin galectin 9 via induction of beta interferon.
    Authors: McSharry B, Forbes S, Cao J, Avdic S, Machala E, Gottlieb D, Abendroth A, Slobedman B
    J Virol, 2014;88(18):10990-4.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. TIM-3 does not act as a receptor for galectin-9.
    Authors: Leitner, Judith, Rieger, Armin, Pickl, Winfried, Zlabinger, Gerhard, Grabmeier-Pfistershammer, Katharin, Steinberger, Peter
    PLoS Pathog, 2013;9(3):e1003253.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  8. Extended co-expression of inhibitory receptors by human CD8 T-cells depending on differentiation, antigen-specificity and anatomical localization.
    Authors: Baitsch L, Legat A, Barba L, Fuertes Marraco SA, Rivals JP, Baumgaertner P, Christiansen-Jucht C, Bouzourene H, Rimoldi D, Pircher H, Rufer N, Matter M, Michielin O, Speiser DE
    PLoS ONE, 2012;7(2):e30852.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  9. Upregulation of the tim-3/galectin-9 pathway of T cell exhaustion in chronic hepatitis B virus infection.
    Authors: Nebbia G, Peppa D, Schurich A, Khanna P, Singh H, Cheng Y, Rosenberg W, Dusheiko G, Gilson R, Chinaleong J, Kennedy P, Maini M
    PLoS ONE, 2012;7(10):e47648.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  10. A crucial role for Kupffer cell-derived galectin-9 in regulation of T cell immunity in hepatitis C infection.
    Authors: Mengshol JA, Golden-Mason L, Arikawa T
    PLoS ONE, 2010;5(3):e9504.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  11. The galectin profile of the endothelium: altered expression and localization in activated and tumor endothelial cells.
    Authors: Thijssen VL, Hulsmans S, Griffioen AW
    Am. J. Pathol., 2008;172(2):545-53.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Expand to show all 11 Citations
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