Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Binding Assay, Cell Culture

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Galectin-9 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Galectin-9

Specificity

Detects human Galectin-9 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Galectin-3 and rhGalectin-4 is observed and less than 2% cross-reactivity with rhGalectin-1, rhGalectin-2, rhGalectin-7, and rhGalectin-8 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Galectin‑9 Antibody

Detection of Human Galectin-9 antibody by Western Blot.

Detection of Human Galectin‑9 by Western Blot.

Western blot shows conditioned media from HT-29 human colon adenocarcinoma cell line, human peripheral blood lymphocytes (PBL), and human dendritic cells untreated (-) or treated (+) with 10 µg/mL PHA for 6 days. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2045) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Galectin-9 at approximately 36 and 40 kDa. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Galectin-9 antibody in Human PBMCs by Immunocytochemistry (ICC).

Galectin‑9 in Human PBMCs.

Galectin-9 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with Calcium Ionomycin and PMA using Goat Anti-Human Galectin-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2045) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Galectin-9 antibody in Human Colon by Immunohistochemistry (IHC-P).

Galectin‑9 in Human Colon.

Galectin-9 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human Galectin-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2045) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Galectin‑9 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with Calcium Ionomycin and PMA

Immunohistochemistry

0.3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon

Western Blot

1 µg/mL
Sample: HT‑29 human colon adenocarcinoma cell line, human peripheral blood lymphocytes (PBL), and human dendritic cells treated with PHA

Reviewed Applications

Read 3 reviews rated 4.7 using AF2045 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Galectin-9

Galectin-9 (also known as Ecalectin and UAT) is a 40 kDa beta -galactosidase binding lectin that belongs to a tandem repeat group of Galectins. Activated cells such as T cells, PMNs, astrocytes, fibroblasts and endothelial cells can express Galectin-9, but its release requires select MMPs. Human to mouse, there is 69% aa sequence identity for Galectin-9.

Alternate Names

Ecalectin, GAL9, Galectin9, LGALS9

Entrez Gene IDs

3965 (Human); 16859 (Mouse)

Gene Symbol

LGALS9

Additional Galectin-9 Products

Product Documents for Human Galectin‑9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Galectin‑9 Antibody

For research use only

Citations for Human Galectin‑9 Antibody

Customer Reviews for Human Galectin‑9 Antibody (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human Galectin-9 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Verified Customer | Posted 01/24/2024
    Human Galectin‑9 Antibody AF2045
  • Human Galectin-9 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Breast cancer tissue
    Species: Human
    Verified Customer | Posted 04/04/2020
    Human Galectin‑9 Antibody AF2045
  • Human Galectin-9 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: SK-BR-3 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 10/28/2018
    Human Galectin‑9 Antibody AF2045

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Protocols

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