Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all gamma-Glutamylcyclotransferase/CRF21 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human gamma ‑Glutamylcyclotransferase/CRF21
Met1-Leu188
Accession # O75223

Specificity

Detects human gamma ‑Glutamylcyclotransferase/CRF21 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human gamma-Glutamylcyclotransferase/CRF21 Antibody

Detection of Human ?-Glutamylcyclotransferase/CRF21 antibody by Western Blot.

Detection of Human gamma ‑Glutamylcyclotransferase/CRF21 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human gamma-Glutamylcyclotransferase/CRF21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5086) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for gamma-Glutamylcyclotransferase/CRF21 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human gamma-Glutamylcyclotransferase/CRF21 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: gamma-Glutamylcyclotransferase/CRF21

Gamma-glutamylcyclotransferase  (GGCT; also Cytochrome c-releasing factor 21, CRF21, C7orf24 or LOC79017) is a 21 kDa AIG2-like family member. It is a cytosolic protein that induces the release of Cytochrome c from mitochondria, possibly initiating apoptosis. Human CRF21 is 188 amino acids (aa) in length and is a complex of beta -sheets and alpha -helices that create two terminal domains. It has been proposed that CRF21 forms homodimers. There are at least two possible splice variants of human CRF21, one showing a 70 aa substitution, while the second variant shows an 18 aa substitution for aa 97‑188. Full-length human CRF21 is 82% aa identical to mouse CRF21.

Long Name

Cytochrome c Releasing Factor 21

Alternate Names

C7orf24, CRF21, gammaGlutamylcyclotransferase, GCTG, GGC, GGCT, LOC79017

Entrez Gene IDs

79017 (Human); 110175 (Mouse); 685702 (Rat)

Gene Symbol

GGCT

UniProt

O75223 (Human)

Additional gamma-Glutamylcyclotransferase/CRF21 Products

Product Documents for Human gamma-Glutamylcyclotransferase/CRF21 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human gamma-Glutamylcyclotransferase/CRF21 Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Human gamma-Glutamylcyclotransferase/CRF21 Antibody

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