Human gamma-Glutamylcyclotransferase/CRF21 Antibody

Catalog # Availability Size / Price Qty
Detection of Human  gamma ‑Glutamylcyclotransferase/CRF21 by Western Blot.
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Product Details
Citations (1)
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Human gamma-Glutamylcyclotransferase/CRF21 Antibody Summary

Species Reactivity
Detects human gamma ‑Glutamylcyclotransferase/CRF21 in Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human gamma ‑Glutamylcyclotransferase/CRF21
Accession # O75223
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ?-Glutamylcyclotransferase/CRF21 antibody by Western Blot. View Larger

Detection of Human gamma ‑Glutamylcyclotransferase/CRF21 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human gamma-Glutamylcyclotransferase/CRF21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5086) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for gamma-Glutamylcyclotransferase/CRF21 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: gamma-Glutamylcyclotransferase/CRF21

Gamma-glutamylcyclotransferase  (GGCT; also Cytochrome c-releasing factor 21, CRF21, C7orf24 or LOC79017) is a 21 kDa AIG2-like family member. It is a cytosolic protein that induces the release of Cytochrome c from mitochondria, possibly initiating apoptosis. Human CRF21 is 188 amino acids (aa) in length and is a complex of beta -sheets and alpha -helices that create two terminal domains. It has been proposed that CRF21 forms homodimers. There are at least two possible splice variants of human CRF21, one showing a 70 aa substitution, while the second variant shows an 18 aa substitution for aa 97‑188. Full-length human CRF21 is 82% aa identical to mouse CRF21.

Long Name
Cytochrome c Releasing Factor 21
Entrez Gene IDs
79017 (Human); 110175 (Mouse); 685702 (Rat)
Alternate Names
C7orf24; CRF21; gammaGlutamylcyclotransferase; gamma-Glutamylcyclotransferase; GCTG; GGC; GGCT; LOC79017; MGC3077

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Citation for Human gamma-Glutamylcyclotransferase/CRF21 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Prevention of tumor growth by needle-free jet injection of anti-C7orf24 siRNA.
    Authors: Hama S, Arata M, Nakamura I
    Cancer Gene Ther., 2012-06-01;19(8):553-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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