Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Xenograft

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot, Immunocytochemistry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all GATA-6 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GATA-6
Met1-Ala449
Accession # Q92908-2

Specificity

Detects human GATA-6.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human GATA‑6 Biotinylated Antibody

GATA‑6 antibody in KATO-III Human Cell Line by Immunocytochemistry (ICC).

GATA‑6 in KATO-III Human Cell Line.

GATA‑6 was detected in immersion fixed KATO-III human gastric carcinoma cell line using 10 µg/mL Human GATA‑6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1700) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red, upper panel; Catalog # NL999) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human GATA‑6 Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed KATO-III human gastric carcinoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human GATA‑6

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GATA-6

GATA-6 (also named GATA-GT1) is a zinc finger transcription activating protein that bins to a T/A-G-A-T-A-G/A DNA sequence motif in mesodermally and endodermally-derived tissue. There are two GATA-6 isoforms that arise from alternate start sites: a short form of 449 amino acids (aa), and a long form of 595 aa. The short form of human GATA-6 is 93%, 92% and 88% aa identical to GATA-6 in porcine, rat and mouse, respectively.

Alternate Names

GATA6

Entrez Gene IDs

2627 (Human)

Gene Symbol

GATA6

UniProt

Q92908-2

Additional GATA-6 Products

Product Documents for Human GATA‑6 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GATA‑6 Biotinylated Antibody

For research use only

Citations for Human GATA‑6 Biotinylated Antibody

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Protocols

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