GATA‑6 in KATO-III Human Cell Line. GATA‑6 was detected in immersion fixed KATO-III human gastric carcinoma cell line using 10 µg/mL Human GATA‑6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1700) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red, upper panel; Catalog # NL999) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
GATA-6 (also named GATA-GT1) is a zinc finger transcription activating
protein that bins to a T/A-G-A-T-A-G/A DNA sequence motif in
mesodermally and endodermally-derived tissue. There are two GATA-6
isoforms that arise from alternate start sites: a short form of 449
amino acids (aa), and a long form of 595 aa. The short form of human
GATA-6 is 93%, 92% and 88% aa identical to GATA-6 in porcine, rat and
Entrez Gene IDs:
GATA binding protein 6; GATA6; GATA-binding factor 6; GATA-binding protein 6; transcription factor GATA-6