Human GATA-6 Antibody

(18 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human GATA-6 in direct ELISAs and Western blots.  In direct ELISAs, approximately 5% cross-reactivity with recombinant human GATA-5 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human GATA-6
    Met1-Thr449
    Accession # Q92908
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Chromatin Immunoprecipitation (ChIP)
    5 µg/5 x 106 cells
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human GATA‑6 by Western Blot. Western blot shows cytoplasmic and nuclear extracts from PC‑3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GATA‑6 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Chromatin Immunoprecipitation (ChIP)
Detection of GATA‑6-regulated Genes by Chromatin Immunoprecipitation. KATO‑III human gastric carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GATA‑6/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The mucin4 promoter was detected by standard PCR.
Immunocytochemistry
GATA‑6 in KATO-III Human Cell Line. GATA‑6 was detected in immersion fixed KATO-III human gastric carcinoma cell line using 10 µg/mL Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
GATA‑6 in Human Intestine. GATA‑6 was detected in immersion fixed paraffin-embedded sections of human intestine using Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GATA-6
GATA-6 (also named GATA-GT1) is a zinc finger transcription activating protein that bins to a T/A-G-A-T-A-G/A DNA sequence motif in mesodermally and endodermally-derived tissue. There are two GATA-6 isoforms that arise from alternate start sites: a short form of 449 amino acids (aa), and a long form of 595 aa. The short form of human GATA-6 is 93%, 92% and 88% aa identical to GATA-6 in porcine, rat and mouse, respectively.
  • Entrez Gene IDs:
    2627 (Human)
  • Alternate Names:
    GATA binding protein 6; GATA6; GATA-6; GATA-binding factor 6; GATA-binding protein 6; transcription factor GATA-6
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Direct Reprogramming of Fibroblasts via a Chemically Induced XEN-like State
    Authors: X Li, D Liu, Y Ma, X Du, J Jing, L Wang, B Xie, D Sun, S Sun, X Jin, X Zhang, T Zhao, J Guan, Z Yi, W Lai, P Zheng, Z Huang, Y Chang, Z Chai, J Xu, H Deng
    Cell Stem Cell, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  2. Dynamic Heterogeneity of Brachyury in Mouse Epiblast Stem Cells Mediates Distinct Response to Extrinsic Bone Morphogenetic Protein (BMP) Signaling
    Authors: L Song, J Chen, G Peng, K Tang, N Jing
    J. Biol. Chem., 2016;291(29):15212-25.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  3. p38 (Mapk14/11) occupies a regulatory node governing entry into primitive endoderm differentiation during preimplantation mouse embryo development
    Open Biol, 2016;6(9):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-Fluorescent
  4. Efficient derivation of extraembryonic endoderm stem cell lines from mouse postimplantation embryos
    Sci Rep, 2016;6(0):39457.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  5. Abrogation Of Gap Junctional Communication In Es Cells Results In A Disruption Of Primitive Endoderm Formation In Embryoid Bodies
    Stem Cells, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Fresh
  6. Asynchronous fate decisions by single cells collectively ensure consistent lineage composition in the mouse blastocyst
    Nat Commun, 2016;7(0):13463.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  7. Inhibition of transforming growth factor beta (TGF-beta) signaling can substitute for Oct4 protein in reprogramming and maintain pluripotency.
    Authors: Tan F, Qian C, Tang K, Abd-Allah S, Jing N
    J Biol Chem, 2015;290(7):4500-11.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. Epigenetic regulation of histone modifications and Gata6 gene expression induced by maternal diet in mouse embryoid bodies in a model of developmental programming.
    Authors: Sun, Congshan, Denisenko, Oleg, Sheth, Bhavwant, Cox, Andy, Lucas, Emma S, Smyth, Neil R, Fleming, Tom P
    BMC Dev Biol, 2015;15(0):3.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  9. GATA family members as inducers for cellular reprogramming to pluripotency.
    Authors: Shu, Jian, Zhang, Ke, Zhang, Minjie, Yao, Anzhi, Shao, Sida, Du, Fengxia, Yang, Caiyun, Chen, Wenhan, Wu, Chen, Yang, Weifeng, Sun, Yingli, Deng, Hongkui
    Cell Res, 2015;25(2):169-80.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP
  10. BMP signalling regulates the pre-implantation development of extra-embryonic cell lineages in the mouse embryo.
    Authors: Graham, Sarah J, Wicher, Krzyszto, Jedrusik, Agnieszk, Guo, Guoji, Herath, Wishva, Robson, Paul, Zernicka-Goetz, Magdalen
    Nat Commun, 2014;5(0):5667.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  11. GATA6 levels modulate primitive endoderm cell fate choice and timing in the mouse blastocyst.
    Authors: Schrode N, Saiz N, Di Talia S, Hadjantonakis A
    Dev Cell, 2014;29(4):454-67.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  12. Formation of a polarised primitive endoderm layer in embryoid bodies requires fgfr/erk signalling.
    Authors: Doughton, Gail, Wei, Jun, Tapon, Nicolas, Welham, Melanie, Chalmers, Andrew D
    PLoS ONE, 2014;9(4):e95434.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  13. Mouse early extra-embryonic lineages activate compensatory endocytosis in response to poor maternal nutrition.
    Authors: Sun, Congshan, Velazquez, Miguel A, Marfy-Smith, Stephani, Sheth, Bhavwant, Cox, Andy, Johnston, David A, Smyth, Neil, Fleming, Tom P
    Development, 2014;141(5):1140-50.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  14. GATA6 Promotes Angiogenic Function and Survival in Endothelial Cells by Suppression of Autocrine Transforming Growth Factor beta/Activin Receptor-like Kinase 5 Signaling.
    Authors: Froese N, Kattih B, Breitbart A, Grund A, Geffers R, Molkentin JD, Kispert A, Wollert KC, Drexler H, Heineke J
    J. Biol. Chem., 2011;286(7):5680-90.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  15. A role for PDGF signaling in expansion of the extra-embryonic endoderm lineage of the mouse blastocyst.
    Authors: Artus J, Panthier JJ, Hadjantonakis AK
    Development, 2010;137(20):3361-72.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  16. A Gata6-Wnt pathway required for epithelial stem cell development and airway regeneration.
    Authors: Zhang Y, Goss AM, Cohen ED, Kadzik R, Lepore JJ, Muthukumaraswamy K, Yang J, DeMayo FJ, Whitsett JA, Parmacek MS, Morrisey EE
    Nat. Genet., 2008;40(7):862-70.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  17. The human mucin MUC4 is transcriptionally regulated by caudal-related homeobox, hepatocyte nuclear factors, forkhead box A, and GATA endodermal transcription factors in epithelial cancer cells.
    Authors: Jonckheere N, Vincent A, Perrais M, Ducourouble MP, Male AK, Aubert JP, Pigny P, Carraway KL, Freund JN, Renes IB, Van Seuningen I
    J. Biol. Chem., 2007;282(31):22638-50.
    Species: Human
    Sample Type: Cell Lysates
    Application: ChIP
  18. New cell lines from mouse epiblast share defining features with human embryonic stem cells.
    Authors: Tesar PJ, Chenoweth JG, Brook FA, Davies TJ, Evans EP, Mack DL, Gardner RL, McKay RD
    Nature, 2007;448(7150):196-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 18 Citations
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Isotype Controls
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human GATA‑6 Antibody AF1700
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Excellent
 ICC/IF Mouse Anonymous 10/26/2015
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Immunocytochemistry/Immunofluorescence Human GATA‑6 Antibody AF1700
Immunocytochemistry/Immunofluorescence: Human GATA‑6 Antibody [AF1700]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedE4.5 mouse embryo
SpeciesMouse

Other Experimental Details

Other Experimental DetailsSpecificity: Specific
Sensitivity: Sensitive
Buffer: PBST
Dilution: 1/500

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